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Angiotensin II type 1 receptor A1166C GENE polymorphism and essential hypertension in San Luis Biocell
Lapierre,Alicia Viviana; Arce,María Elena; Lopez,José Raúl; Ciuffo,Gladys María.
Essential hypertension is considered a multifactorial trait resulting from a combination of environmental and genetic factors. The angiotensin II type 1 receptor mediates the vasoconstrictor and growthpromoting effects of Ang II. The A1166C polymorphism of the AT1 receptor gene may be associated with cardiovascular phenotypes, such as high arterial blood pressure, aortic stiffness, and increased cardiovascular risk. We investigated the association between this A1166C polymorphism and hypertension in hypertense and normotense subjects from San Luis (Argentina) by mismatch PCR-RFLP analysis. Hypertense patients exhibited significant increases in lipid related values and body mass index. The frequency of occurrence of the C1166 allele was higher among...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; A1166C polymorphism; Risk factors; RAS system; Pharmacogenetics.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300005
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Biochemical-molecular markers in unilateral ureteral obstruction Biocell
Manucha,Walter.
Congenital obstructive nephropathy is the primary cause of end-stagerenal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstr uctive uropathy effects-decline in the plasmatic renal flow and glomerular filtrationrate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosisare a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-b (TGF-b) and nitric oxide (NO) have been implicated in congenital obstructive nephropathy. The renin-angiotensin system is regulated in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Unilateral ureteral obstruction; Interstitial fibrosis; Nitric oxide; Angiotensin II; Oxidative stress.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100001
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The effect of losartan on angiotensin II-induced cell proliferation in a rat aorta smooth muscle cell line BABT
Tambelline,Natália; Oliveira,Karen; Olchanheski Junior,Luiz Renato; Sordi,Regina; Otuki,Michel Fleith; Favero,Giovani Marino; Fernandes,Daniel.
The success of revascularization procedures is limited by recurrent stenosis, which is a narrowing of a blood vessels that results from neo-intimal hyperplasia. The renin-angiotensin-aldosterone system has been implicated in the pathogenesis of neo-intimal hyperplasia, and a role for angiotensin II in vascular smooth muscle cell proliferation has been proposed. There are at least two high-affinity subtypes of angiotensin II receptors, AT1 and AT2, both of which are seven-transmembrane G protein-coupled receptors. We investigated the effect of losartan, an AT1 receptor antagonist, on vascular smooth muscle cell proliferation using the A7r5 smooth cell line derived from rat aorta. Losartan was shown to prevent angiotensin II-induced cell proliferation,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Losartan; Cell proliferation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200012
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Alternative pathways for angiotensin II generation in the cardiovascular system BJMBR
Becari,C.; Oliveira,E.B.; Salgado,M.C.O..
The classical renin-angiotensin system (RAS) consists of enzymes and peptides that regulate blood pressure and electrolyte and fluid homeostasis. Angiotensin II (Ang II) is one of the most important and extensively studied components of the RAS. The beneficial effects of angiotensin converting enzyme (ACE) inhibitors in the treatment of hypertension and heart failure, among other diseases, are well known. However, it has been reported that patients chronically treated with effective doses of these inhibitors do not show suppression of Ang II formation, suggesting the involvement of pathways alternative to ACE in the generation of Ang II. Moreover, the finding that the concentration of Ang II is preserved in the kidney, heart and lungs of mice with an ACE...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Renin-angiotensin system; Angiotensin II; Rat elastase-2; Alternative pathway; Angiotensin-converting enzyme.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900013
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Angiotensin II induces NF-κB, JNK and p38 MAPK activation in monocytic cells and increases matrix metalloproteinase-9 expression in a PKC- andRho kinase-dependent manner BJMBR
Yaghooti,H.; Firoozrai,M.; Fallah,S.; Khorramizadeh,M.R..
Angiotensin II (ANG II), the main effector of the renin-angiotensin system, is implicated in endothelial permeability, recruitment and activation of the immune cells, and also vascular remodeling through induction of inflammatory genes. Matrix metalloproteinases (MMPs) are considered to be important inflammatory factors. Elucidation of ANG II signaling pathways and of possible cross-talks between their components is essential for the development of efficient inhibitory medications. The current study investigates the inflammatory signaling pathways activated by ANG II in cultures of human monocytic U-937 cells, and the effects of specific pharmacological inhibitors of signaling intermediates on MMP-9 gene (MMP-9) expression and activity. MMP-9 expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Signaling; MMP-9; Monocytic cell.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300003
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Role of endothelium in angiotensin II formation by the rat aorta and mesenteric arterial bed BJMBR
We investigated the angiotensin II (Ang II)-generating system by analyzing the vasoconstrictor effect of Ang II, angiotensin I (Ang I), and tetradecapeptide (TDP) renin substrate in the absence and presence of inhibitors of the renin-angiotensin system in isolated rat aortic rings and mesenteric arterial beds with and without functional endothelium. Ang II, Ang I, and TDP elicited a dose-dependent vasoconstrictor effect in both vascular preparations that was completely blocked by the Ang II receptor antagonist saralasin (50 nM). The angiotensin converting enzyme (ACE) inhibitor captopril (36 µM) completely inhibited the vasoconstrictor effect elicited by Ang I and TDP in aortic rings without affecting that of Ang II. In contrast, captopril (36 µM)...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Angiotensin II; Angiotensin converting enzyme; Renin-angiotensin system; Endothelium; Blood vessels; Captopril.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500013
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Angiotensin and baroreflex control of the circulation BJMBR
Head,G.A.; Saigusa,T.; Mayorov,D.N..
There is a close association between the location of angiotensin (Ang) receptors and many important brain nuclei involved in the regulation of the cardiovascular system. The present review encompasses the physiological role of Ang II in the brainstem, particularly in relation to its influence on baroreflex control of the heart and kidney. Activation of AT1 receptors in the brainstem by fourth ventricle (4V) administration to conscious rabbits or local administration of Ang II into the rostral ventrolateral medulla (RVLM) of anesthetized rabbits acutely increases renal sympathetic nerve activity (RSNA) and RSNA baroreflex responses. Administration of the Ang antagonist Sarile into the RVLM of anesthetized rabbits blocked the effects of Ang II on the RSNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; AT1 receptors; Losartan; Renal sympathetic activity; Baroreflex; Rostral ventrolateral medulla; Blood pressure; Rabbit.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900005
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Angiotensin II-mediated vascular smooth muscle cell growth signaling BJMBR
Inagami,T.; Eguchi,S..
The mechanism by which Ang II stimulates the growth of vascular smooth muscle cells was investigated by measuring the phosphorylation of mitogen-activated protein kinases ERK 1 and ERK 2. Ca2+ ionophore was found to have effects practically analogous to Ang II. We found that the signaling pathway involves the activation of epidermal growth factor receptor (EGFR) kinase, activation of the adaptor proteins Shc and Grb2, and the small G-protein Ras. Although the mechanism of AT1- (or Ca2+)-induced activation of EGFR is not yet clear, we have found that calcium-dependent protein kinase CAKß/PYK2 and c-Src are involved in this process. These studies indicate a transactivation mechanism that utilizes EGFR as a bridge between a Gq-coupled receptor and activation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Type 1 receptor; Epidermal growth factor receptor; Vascular smooth muscle cells.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600002
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Sex differences in angiotensin II- induced hypertension BJMBR
Xue,B.; Johnson,A.K.; Hay,M..
Sex differences in the development of hypertension and cardiovascular disease have been described in humans and in animal models. In this paper we will review some of our studies which have as their emphasis the examination of the role of sex differences and sex steroids in modulating the central actions of angiotensin II (ANG II) via interactions with free radicals and nitric oxide, generating pathways within brain circumventricular organs and in central sympathomodulatory systems. Our studies indicate that low-dose infusions of ANG II result in hypertension in wild-type male mice but not in intact wild-type females. Furthermore, we have demonstrated that ANG II-induced hypertension in males is blocked by central infusions of the androgen receptor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Estrogen; Estrogen a and ß receptors; Nitric oxide; Reactive oxygen species; Testosterone.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500018
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A connection between extracellular matrix and hormonal signals during the development of the human fetal adrenal gland BJMBR
Chamoux,E.; Otis,M.; Gallo-Payet,N..
The human adrenal cortex, involved in adaptive responses to stress, body homeostasis and secondary sexual characters, emerges from a tightly regulated development of a zone-specific secretion pattern during fetal life. Its development during fetal life is critical for the well being of pregnancy, the initiation of delivery, and even for an adequate adaptation to extra-uterine life. As early as from the sixth week of pregnancy, the fetal adrenal gland is characterized by a highly proliferative zone at the periphery, a concentric migration accompanied by cell differentiation (cortisol secretion) and apoptosis in the central androgen-secreting fetal zone. After birth, a strong reorganization occurs in the adrenal gland so that it better fulfills the newborn's...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adrenal gland; Development; Human fetus; Extracellular matrix; Angiotensin II; Adrenocorticotropin.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000006
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Renal actions of angiotensin-(1-7) BJMBR
The heptapeptide angiotensin-(1-7) is considered to be a biologically active endproduct of the renin-angiotensin system. This angiotensin, which is devoid of the most known actions of angiotensin II such as induction of drinking behavior and vasoconstriction, has several selective effects in the brain and periphery. In the present article we briefly review recent evidence for a physiological role of angiotensin-(1-7) in the control of hydroelectrolyte balance
Tipo: Info:eu-repo/semantics/other Palavras-chave: Angiotensin antagonists; Angiotensin II; Hydroelectrolyte balance; Renin-angiotensin system; Kidney; Water transport.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400012
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Roles of mitogen-activated protein kinases and angiotensin II in renal development BJMBR
Balbi,A.P.C.; Francescato,H.D.C.; Marin,E.C.S.; Costa,R.S.; Coimbra,T.M..
Experimental and clinical evidence suggests that angiotensin II (AII) participates in renal development. Renal AII content is several-fold higher in newborn rats and mice than in adult animals. AII receptors are also expressed in higher amounts in the kidneys of newborn rats. The kidneys of fetuses whose mother received a type 1 AII receptor (AT1) antagonist during gestation present several morphological alterations. Mutations in genes that encode components of the renin-angiotensin system are associated with autosomal recessive renal tubular dysgenesis. Morphological changes were detected in the kidneys of 3-week-old angiotensin-deficient mice. Mitogen-activated protein kinases (MAPKs) are important mediators that transduce extracellular stimuli to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Renal development; Angiotensin II; Mitogen-activated protein kinase; Angiotensin II antagonists; Sodium intake.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100007
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The hyperglycemia induced by angiotensin II in rats is mediated by AT1 receptors BJMBR
Machado,L.J.C.; Marubayashi,U.; Reis,A.M.; Coimbra,C.C..
We have shown that the renin-angiotensin system (RAS) is involved in glucose homeostasis during acute hemorrhage. Since almost all of the physiological actions described for angiotensin II were mediated by AT1 receptors, the present experiments were designed to determine the participation of AT1 receptors in the hyperglycemic action of angiotensin II in freely moving rats. The animals were divided into two experimental groups: 1) animals submitted to intravenous administration of angiotensin II (0.96 nmol/100 g body weight) which caused a rapid increase in plasma glucose reaching the highest values at 5 min after the injection (33% of the initial values, P<0.01), and 2) animals submitted to intravenous administration of DuP-753 (losartan), a...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Glycemia; Angiotensin II; DuP-753; AT1; Receptor.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000018
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Gadolinium increases the vascular reactivity of rat aortic rings BJMBR
Angeli,J.K.; Ramos,D.B.; Casali,E.A.; Souza,D.O.G.; Sarkis,J.J.F.; Stefanon,I.; Vassallo,D.V.; Fürstenau,C.R..
Gadolinium (Gd) blocks intra- and extracellular ATP hydrolysis. We determined whether Gd affects vascular reactivity to contractile responses to phenylephrine (PHE) by blocking aortic ectonucleoside triphosphate diphosphohydrolase (E-NTPDase). Wistar rats of both sexes (260-300 g, 23 females, 7 males) were used. Experiments were performed before and after incubation of aortic rings with 3 µM Gd. Concentration-response curves to PHE (0.1 nM to 0.1 mM) were obtained in the presence and absence of endothelium, after incubation with 100 µM L-NAME, 10 µM losartan, or 10 µM enalaprilat. Gd significantly increased the maximum response (control: 72.3 ± 3.5; Gd: 101.3 ± 6.4%) and sensitivity (control: 6.6 ± 0.1; Gd: 10.5 ± 2.8%) to PHE. To investigate the blockade...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gadolinium; E-NTPDase; Adenosine; Angiotensin II; AT1receptor.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000500010
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Antihypertensive effects of angiotensin-(1-7) BJMBR
Chappell,M.C.; Iyer,S.N.; Diz,D.I.; Ferrario,C.M..
Accumulating evidence suggests that angiotensin-(1-7) (Ang-(1-7)) is an important component of the renin-angiotensin system and that the actions of the peptide may either contribute to or oppose those of Ang II. Ang-(1-7) can be converted directly from Ang I bypassing prerequisite formation of Ang II. Formation of Ang-(1-7) is under the control of at least three endopeptidases depending on the tissue compartment and include neprilysin, thimet oligopeptidase and prolyl oligopeptidase. Both neprilysin and thimet oligopeptidase are also involved in the metabolism of bradykinin and the atrial natriuretic peptide. Moreover, recent studies suggest that in addition to Ang I and bradykinin, Ang-(1-7) is an endogenous substrate for angiotensin converting enzyme....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin-(1-7); Angiotensin II; Hypertension.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900014
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Dissociation between the circulating renin-angiotensin system and angiotensin II receptors in central losartan-induced hypertension BJMBR
Sugawara,A.M.; Vendramini,R.C.; Barbosa,S.P.; Brunetti,I.L.; Menani,J.V.; De Luca Jr.,L.A..
Losartan, an AT1 angiotensin II (ANG II) receptor non-peptide antagonist, induces an increase in mean arterial pressure (MAP) when injected intracerebroventricularly (icv) into rats. The present study investigated possible effector mechanisms of the increase in MAP induced by icv losartan in unanesthetized rats. Male Holtzman rats (280-300 g, N = 6/group) with a cannula implanted into the anterior ventral third ventricle received an icv injection of losartan (90 µg/2 µl) that induced a typical peak pressor response within 5 min. In one group of animals, this response to icv losartan was completely reduced from 18 ± 1 to 4 ± 2 mmHg by intravenous (iv) injection of losartan (2.5-10 mg/kg), and in another group, it was partially reduced from 18 ± 3 to 11 ± 2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Alpha1-Adrenoceptors; Arterial pressure; AT1 receptors; Angiotensin-converting enzyme; Renin.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900007
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Adrenoceptors of the medial septal area modulate water intake and renal excretory function induced by central administration of angiotensin II BJMBR
Saad,W.A.; Guarda,I.F.M.S.; Camargo,L.A.A.; Santos,T.A.F.B.; Simões,S.; Saad,Willian A..
We investigated the role of a-adrenergic antagonists and clonidine injected into the medial septal area (MSA) on water intake and the decrease in Na+, K+ and urine elicited by ANGII injection into the third ventricle (3rdV). Male Holtzman rats with stainless steel cannulas implanted into the 3rdV and MSA were used. ANGII (12 nmol/µl) increased water intake (12.5 ± 1.7 ml/120 min). Clonidine (20 nmol/µl) injected into the MSA reduced the ANGII-induced water intake (2.9 ± 0.5 ml/120 min). Pretreatment with 80 nmol/µl yohimbine or prazosin into the MSA also reduced the ANGII-induced water intake (3.0 ± 0.4 and 3.1 ± 0.2 ml/120 min, respectively). Yohimbine + prazosin + clonidine injected into the MSA abolished the ANGII-induced water intake (0.2 ± 0.1 and 0.2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: A-Adrenoceptors; Angiotensin II; Water intake; Sodium; Potassium; Water excretion; Medial septal area.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800012
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Ligand-induced endocytosis and nuclear localization of angiotensin II receptors expressed in CHO cells BJMBR
Merjan,A.J.; Kanashiro,C.A.; Krieger,J.E.; Han,S.W.; Paiva,A.C.M..
A construct (AT1R-NF) containing a "Flag" sequence added to the N-terminus of the rat AT1 receptor was stably expressed in Chinese hamster ovary cells and quantified in the cell membrane by confocal microscopy after reaction with a fluorescein-labeled anti-Flag monoclonal antibody. Angiotensin II bound to AT1R-NF and induced endocytosis with a half-time of 2 min. After 60-90 min, fluorescence accumulated around the cell nucleus, suggesting migration of the ligand-receptor complex to the nuclear membrane. Angiotensin antagonists also induced endocytosis, suggesting that a common step in the transduction signal mechanism occurring after ligand binding may be responsible for the ligand-receptor complex internalization.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Agonist-induced receptor internalization; Endocytosis; Fluorescence-tagged receptor; Nuclear angiotensin receptors; Renin-angiotensin system.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900011
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Neuroendocrine control of body fluid homeostasis BJMBR
McCann,S.M.; Gutkowska,J.; Antunes-Rodrigues,J..
Angiotensin II and atrial natriuretic peptide (ANP) play important and opposite roles in the control of water and salt intake, with angiotensin II promoting the intake of both and ANP inhibiting the intake of both. Following blood volume expansion, baroreceptor input to the brainstem induces the release of ANP within the hypothalamus that releases oxytocin (OT) that acts on its receptors in the heart to cause the release of ANP. ANP activates guanylyl cyclase that converts guanosine triphosphate into cyclic guanosine monophosphate (cGMP). cGMP activates protein kinase G that reduces heart rate and force of contraction, decreasing cardiac output. ANP acts similarly to induce vasodilation. The intrinsic OT system in the heart and vascular system augments the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oxytocin; Vasopressin; Atrial natriuretic peptide; Nitric oxide; Angiotensin II; Guanylyl cyclase.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200003
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Central lead administration inhibits water intake and sodium appetite in rats BJMBR
De-Castro-e-Silva,E.J.; Castro,L.; Luz,C.P.; Ferreira,H.; Lima,A.K.S.; Souza,F.S.F.; Maldonado,I.; Macêdo,D.F.; Ferreira,M.G.; Bandeira,I.P.V.; Amor,A.L.M.; Carvalho,F.L.Q.; Rocha Jr.,M.A.; Fregoneze,J.B..
We have demonstrated that acute third ventricle injections of lead acetate (PbAc) exert a powerful antidipsogenic effect and induce a significant increase in renal sodium excretion. In the present study we confirm the antidipsogenic effect of lead and demonstrate that central administration of this metal, in minute amounts, significantly reduces salt intake both during dehydration and after central angiotensinergic stimulation. Adult male Wistar rats had the third ventricle cannulated seven days before the experiments. During this period they had free access to distilled water and hypertonic saline solution (1.5%). After a 24-h period of fluid deprivation, experimental animals received third ventricle injections of PbAc (0.3, N = 8 and 3.0 nmol/rat, N =...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Lead; Angiotensin II; Water intake; Sodium intake; Rats.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000011
Registros recuperados: 32
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