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Registros recuperados: 5
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DNA testing for parentage verification in a conservation nucleus of Pantaneiro horse Genet. Mol. Biol.
Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Delgado,Juan Vicente.
We investigated the genealogy of the in situ conservation nucleus of the Pantaneiro horse using DNA microsatellites by evaluating 101 horses, the group consisting of 71 adult horses (3 stallions, 40 male and 31 mares) and 27 foals (14 colts and 13 fillies). Genomic DNA was extracted from hair roots and genotyped using 12 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, HMS3 HMS6, HMS7, HTG4, HTG10, LEX33 and VHL20). The number of alleles per locus varied from 6 to 13, with a mean of 7.8 and the expected heterozygosity ranged from 0.544 to 0.734 (mean 0.644). The VLH20, ASB2, HTG10, ASB23 markers had a high (> 0.8) polymorphism information content and the total exclusion probability of the 12 microsatellite loci was 0.99. The genealogical study of...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Pantaneiro horse; Animal conservation; Microsatellites; Markers; Parentage verification.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100013
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Isolamento, cultivo criopreservação de células somáticas de mamíferos silvestres para formação de um banco de germoplasma. Infoteca-e
MARTINS, C. F.; CUMPA, H. C. B.; CUNHA, E. R. da; SILVA, C. G. da; GAMA FILHO, J. B. da; BORGES, L. A..
bitstream/item/100353/1/comtec-175.pdf
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Animal silvestre; Animal conservation; Genebank.; Criopreservação; Germoplasma; Mamífero.; Biotechnology; Cryopreservation; Reproduction; Wild animals..
Ano: 2013 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/981278
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Genetic diversity and population structure of Brazilian native bovine breeds PAB
Serrano,Germana Maria; Egito,Andréa Alves do; McManus,Concepta; Mariante,Arthur da Silva.
Conservation and improvement strategies should be based on the association between genetic and phenotypic characteristics. The objective of this work was to characterize five native Brazilian cattle breeds (Caracu, Crioulo Lageano, Curraleiro, National Polled and Pantaneiro) and two commercial breeds (Holstein and Nellore) using RAPD technique to estimate genetic distances and variability between and within breeds. Genetic relationships were investigated using 22 primers which generated 122 polymorphic bands. Analysis of molecular variance indicated that most of the genetic variation lay among individuals within populations. The genetic variabilities between pairs of breeds were statistically significant. The smallest genetic divergence was between Crioulo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal conservation; Cattle; Genetic characterization; RAPD.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000600005
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Genetic diversity of Brazilian Pantaneiro horse and relationships among horse breeds PAB
Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Kelly,Lucy; Bermejo,Juan Vicente Delgado.
The objective of this work was to evaluate the genetic diversity of Brazilian Pantaneiro horse by microsatellite markers, investigate the effect of genetic bottlenecks and estimate genetic differentiation among four horse breeds. Genetic variation was estimated through allele frequencies and mean breed heterozygosity. Nei's genetic distances among the breeds Pantaneiro, Thoroughbred, Arabian, Spanish Pure Breed (Andalusian), and Uruguay Creole were calculated, and it was used to construct an UPGMA dendrogram. Clustering at different K values was calculated to infer population structure and assign individuals to populations. Nei's distances showed a minimum distance between Pantaneiro horse and Spanish Pure Breed (0.228), and similar distances from Spanish...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equus caballus; Animal conservation; Molecular markers; Microsatellites; Genetic resources conservation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500007
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Genetic diversity of captive spotted paca (Agouti paca) from south east Brazil assessed by the RAPD-PCR technique R. Bras. Zootec.
Antunes,Karine Vieira; Machado,Théa Mírian Medeiros; Serão,Nicola Vergara Lopes; Guimarães,Simone Eliza Facione; Paiva,Samuel Rezende.
The genetic diversity was analyzed among spotted paca (Agouti paca) from three commercial flocks located in Brazil. As the genome of this species is unknown, the RAPD-PCR technique was used. Ten primers generated sixty polymorphic bands. The among and within population genetic variability estimated by analysis of molecular variance (AMOVA) was 12.55 and 87.45%, respectively. The shortest Nei distance value was 11.76% among the Carangola (CG) and São Francisco do Glória (SF) populations. This value can be explained by the exchange of reproduction males and females between the two geographically close breeding sites. The analysis of principal components showed well defined and structured groups aggregating animals according their population of origin, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal conservation; Animal genetic resources; Biodiversity; Genetic distance; Wild animals.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000200006
Registros recuperados: 5
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