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Interactions between carpenter bees and orchid bees (Hymenoptera: Apidae) in flowers of Bertholletia excelsa Bonpl. (Lecythidaceae) Acta Amazonica
Santos,Charles Fernando dos; Absy,Maria Lúcia.
Competition between two species of bees for the same type of floral resource may generate antagonistic behavior between them, especially in cultivated areas where food resources are limited, seasonally and locally. In this study, was tested the hypothesis of antagonism between two solitary bee species of the family Apidae, Eulaema mocsaryi (Euglossini) and Xylocopa frontalis (Xylocopini), visiting the Brazil nut flowers (Bertholletia excelsa: Lecythidaceae) in a central Amazonia agricultural area. The visitation time was analyzed to detect the possible temporal overlap in the foraging of these bees. Furthermore, was analyzed their interspecific interactions for manipulating flower species visited by an opponent species, as well as attempts to attack this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Behaviors; Facilitation; Resource partitioning; Pollinators.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672012000100011
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THE IMPORTANCE OF ANTIMICROBIAL COMPOUNDS PRODUCED BY BENEFICIAL BACTERIA ON THE BIOCONTROL OF PHYTOPATHOGENS Acta biol.Colomb.
CESA-LUNA,Catherine; BAEZ,Antonino; QUINTERO-HERNÁNDEZ,Verónica; CRUZ-ENRÍQUEZ,Joel DE LA; CASTAÑEDA-ANTONIO,Ma Dolores; MUÑOZ-ROJAS,Jesús.
ABSTRACT Bacteria produce antimicrobial compounds to compete for nutrients and space in a particular habitat. Antagonistic interactions can be evaluated by several methodologies including the double-layer agar and simultaneous inhibition assays. Among the well-known inhibitory substances produced by bacteria are the broad-spectrum antibiotics, organic acids, siderophores, antifungal, and bacteriocins. The most studied bacterial genera able to produce these inhibitory substances are Enterococcus, Lactococcus, Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia, and Burkholderia. Some beneficial bacteria can promote plant growth and degrade toxic compounds in the environment representing an attractive solution to diverse issues in agriculture and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Antibiotic; Competition; Inhibition; PGPR.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000100140
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ANTIFUNGAL ACTIVITY SCREENING OF ANTARCTIC ACTINOBACTERIA AGAINST PHYTOPATHOGENIC FUNGI Acta biol.Colomb.
SANTOS,Andrés; NÚÑEZ-MONTERO,Kattia; LAMILLA,Claudio; PAVEZ,Mónica; QUEZADA-SOLÍS,Damián; BARRIENTOS,Leticia.
ABSTRACT The extreme weather conditions in the Antarctic have exerted selective pressures favoring differential features in bacteria to survive this untapped environment (i.e., antibiotic molecules). Notably, higher chances of antibiotic discovery from extremophiles have been proposed recently. Although new organic and environmentally friendly sources for helping in the control of plant pathogenic fungi are necessary, the information about anti-phytopathogenic applications of extremophile microorganisms from untapped environments is limited. In this study, we determined the antifungal effect of actinobacterial strains isolated from Antarctic soils and sediments. Co-culture inhibition assays and Minimum Inhibitory Concentration (MIC) determination revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Antarctic bacteria; Antifungal; Extremophile; Plant pathogen.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000200353
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In vitro assessment of the probiotic potential of lactobacilli isolated from Minas artisanal cheese produced in the Araxá region, Minas Gerais state, Brazil Arq. Bras. Med. Vet. Zootec.
Silva,J.G.; Castro,R.D.; Sant’Anna,F.M.; Barquete,R.M.; Oliveira,L.G.; Acurcio,L.B.; Luiz,L.M.P.; Sales,G.A.; Nicoli,J.R.; Souza,M.R..
ABSTRACT Minas artisanal cheese is made from endogenous starter cultures, including lactic acid bacteria (LAB). Some LAB may possess probiotic potential. Thus, this study aimed to evaluate the in vitro probiotic properties of lactobacilli isolated from Minas artisanal cheeses produced in Minas Gerais. Ten samples of lactobacilli, formerly isolated from those cheeses, were submitted to the following assays: antimicrobial susceptibility, tolerance to artificial gastric juice and biliary salts, production of hydrogen peroxide and antagonism against pathogenic and non-pathogenic micro-organisms. Only L. plantarum (C0) was sensitive to all tested antimicrobials, while the other LAB samples were resistant to at least one drug. Six samples were tolerant to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artisanal cheeses; Beneficial micro-organisms; Antibiogram; Tolerance to gastric juice and biliary salts; Antagonism.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200647
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Antifungal potential of crude extracts of Trichoderma spp. Biota Neotropica
Marques,Eder; Martins,Irene; Mello,Sueli Correa Marques de.
Abstract Antibiosis is the mechanism by which certain microorganisms respond to the presence of others, secreting compounds or metabolites capable of inhibiting or impeding their development. The crude extract of Trichoderma contains a mixture of secondary compounds, which may show antibiotic effect, and has been used for the prospect of this fungus for biological control and other industrial purposes. Faced with the increasing demand of agriculture for ecologically compatible alternatives for the management of diseases, this work aimed to investigate the spectrum of action of Non-Volatile Metabolites (NVMs) of Trichoderma isolates against different plant pathogenic fungi. The antagonistic potential of NVMs was evaluated through the incorporation method of...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Secondary metabolites; Antagonism; Inhibition of mycelial growth; Plant pathogenic fungi.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032018000100503
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Evaluation of antagonistic activity of milk fermented with kefir grains of different origins BABT
Santos,João Paulo Victorino; Araújo,Tatiane Ferreira; Ferreira,Célia Lúcia de Luces Fortes; Goulart,Simone Machado.
In this study, the potential of kefir grains, obtained from three different towns/cities in the Southeast Region of Brazil to inhibit the growth of the five pathogenic microorganisms was evaluated. The samples showed the growth inhibition from 42.80-69.15 for Staphylococcus aureus ATCC 6538, 30.73-59.89 for Escherichia coli ATCC 11229, 44.99-73.05 for Salmonela typhi ATCC 6539, 41.45-54.18 for Listeria monocytogenes ATCC 15313 and 70.38-86.80 for Bacillus cereus RIBO 1222-173-S4. These results indicated that the kefir grains evaluated had antagonistic activity toward the different pathogens tested. The ability to inhibit, although differently depending on the regional microbiota, indicated a potential for their use as a functional food.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kefir; Antagonism; Inhibitors; Functional foods.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500014
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Identification and in vitro production of Lactobacillus antagonists from women with or without bacterial vaginosis BJMBR
Branco,K.M.G.R.; Nardi,R.M.D.; Moreira,J.L.S.; Nunes,A.C.; Farias,L.M.; Nicoli,J.R.; Carvalho,M.A.R..
Lactobacilli isolated from the vaginal tract of women with and without bacterial vaginosis (BV) were identified and characterized for the production of antagonists. Bacterial samples were isolated from healthy women (N = 16), from patients with clinical complaints but without BV (N = 30), and from patients with BV (N = 32). Identification was performed using amplified ribosomal DNA restriction analysis. Production of antagonistic compounds was evaluated by the double-layer diffusion technique using Gram-positive (N = 9) and Gram-negative bacteria (N = 6) as well as yeast (N = 5) as indicator strains. Of a total of 147 isolates, 133 were identified as pertaining to the genus Lactobacillus. Lactobacillus crispatus was the species most frequently recovered,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus; Bacterial vaginosis; Antagonism; Hydrogen peroxide; Identification.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400003
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Characterization of Bacillus isolates of potato rhizosphere from andean soils of Peru and their potential PGPR characteristics BJM
Calvo,Pamela; Ormeño-Orrillo,Ernesto; Martínez-Romero,Esperanza; Zúñiga,Doris.
Bacillus spp. are well known rhizosphere residents of many crops and usually show plant growth promoting (PGP) activities that include biocontrol capacity against some phytopatogenic fungi. Potato crops in the Andean Highlands of Peru face many nutritional and phytophatogenic problems that have a significant impact on production. In this context is important to investigate the natural presence of these microorganisms in the potato rhizosphere and propose a selective screening to find promising PGP strains. In this study, sixty three Bacillus strains isolated from the rhizosphere of native potato varieties growing in the Andean highlands of Peru were screened for in vitro antagonism against Rhizoctonia solani and Fusarium solani. A high prevalence (68%) of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Plant growth-promoting rhizobacteria; Potato; Antagonism; Phosphate solubilization; 16S rRNA gene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400008
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Antibiosis of Trichoderma spp strains native to northeastern Mexico against the pathogenic fungus Macrophomina phaseolina BJM
Mendoza,José Luis Hernández; Pérez,María Isabel Sánchez; Prieto,Juan Manuel González; Velásquez,Jesús DiCarlo Quiroz; Olivares,Jesús Gerardo García; Langarica,Homar Rene Gill.
Abstract Sampling of agricultural soils from the Mexican northeastern region was performed to detect Trichoderma spp., genetically characterize it, and assess its potential use as a biologic control agent against Macrophomina phaseolina. M. phaseolina is a phytopathogen that attacks over 500 species of cultivated plants and causes heavy losses in the regional sorghum crop. Sampling was performed immediately after sorghum or corn harvest in an area that was approximately 170 km from the Mexico-USA border. Sixteen isolates were obtained in total. Using colony morphology and sequencing the internal transcribed spacers (ITS) 1 and 4 of 18S rDNA, 14 strains were identified as Trichoderma harzianum, T. koningiopsis and T. virens. Subsequently, their antagonistic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Macrophomina phaseolina; Antagonism; Hyperparasitism; Trichoderma.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401093
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Antagonistic and protective effects against Salmonella enterica serovar typhimurium by Lactobacillus murinus in the digestive tract of gnotobiotic mice BJM
Vasconcelos,Ana Luiza Sampaio; Nicoli,Jacques Robert; Nardi,Regina Maria Drummond.
In the present study, production of antagonistic substances by Lactobacillus murinus against enteropathogenic bacteria was evaluated in vivo as well as a possible protective effect against an oral challenge with Salmonella Typhimurium using a gnotobiotic animal model. A higher mean survival time (P < 0.05) was observed in L. murinus-associated animals (7.89 ± 3.83 days) when compared with the control ones (4.44 ± 0.73 days). Lactobacillus murinus exerted a potent antagonistic effect in vivo against S. sonnei and to a lesser extent against S. Typhimurium as revealed by inhibitory halos around the feces of Lactobacillus-associated animals. Diameters of inhibitory halos around intestinal contents increased along the intestine tract, following...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Salmonella; Lactobacillus; Gnotobiotic mice.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500007
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Antifungal and proteolytic activities of endophytic fungi isolated from Piper hispidum Sw BJM
Orlandelli,Ravely Casarotti; Almeida,Tiago Tognolli de; Alberto,Raiani Nascimento; Polonio,Julio Cesar; Azevedo,João Lúcio; Pamphile,João Alencar.
Endophytes are being considered for use in biological control, and the enzymes they secrete might facilitate their initial colonization of internal plant tissues and direct interactions with microbial pathogens. Microbial proteases are also biotechnologically important products employed in bioremediation processes, cosmetics, and the pharmaceutical, photographic and food industries. In the present study, we evaluated antagonism and competitive interactions between 98 fungal endophytes and Alternaria alternata, Colletotrichum sp., Phyllosticta citricarpa and Moniliophthora perniciosa. We also examined the proteolytic activities of endophytes grown in liquid medium and conducted cup plate assays. The results showed that certain strains in the assemblage of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Competitive interaction; Dual culture; Cup plate; Protease.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200359
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Partial characterization of antagonistic substance produced by a Clostridium butyricum strain BJM
Caires,Nely Cristina Medeiros; Matos,Aline Magalhães; Farias,Luiz de Macedo; Carvalho,Maria Auxiliadora Roque de; Drummond,Regina Maria Nardi; Nicoli,Jacques Robert; Ribeiro Sobrinho,Antônio Paulino.
The production of antagonistic substance by bacterium present in the infected root canal system (RCS) probably is an important ecological factor for its successful colonization of the local. The objective of this study was to partially characterize an antagonistic substance produced by a Clostridium butyricum isolated from infected RCS.Production of inhibitory compound was evaluated by the agar double layer diffusion technique using Fusobacterium nucleatum and Bifidobacterium adolescentis as indicator bacteria. The physicochemical and biochemical factors tested for the partial characterization were influence of pH and temperature and susceptibility to the action of some proteolytic enzymes. An inhibition zone was observed against the two indicator strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium butyricum; Fusobacterium nucleatum; Bifidobacterium adolescentis; Antagonism; Root canal system.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200014
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Bioassay for selection of biocontroller bacteria against bean common blight (Xanthomonas axonopodis pv. phaseoli) BJM
Zanatta,Zarela G.C.N.; Moura,Andréa B.; Maia,Luciano C.; Santos,André S. dos.
Emphasis has been given on selection of micro-organism for biological control. However, in order to evaluate the biological control potential of a great number of micro-organisms in a small period of time it is necessary to develop an efficient bioassay. Seven hundred and sixty bacterial isolates from different habitats, were selected for compatibility with Rhizobium leguminosarum bv. phaseoli (SEMIA 4077 e SEMIA 4080). Among them 596 isolates were ineffective against both rhizobia. Bean seeds immersed in suspension of each one of these isolates were agitated for 5 hours at 10ºC and sowed in non-sterilized soil. The plants were kept in greenhouse. After the development of cotyledonary and primary leaves, these were removed and bioassayed for Xanthomonas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Antagonism; Phaseolus vulgaris; Microbiolization.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300024
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Molecular identification of Lactobacillus spp. associated with puba, a Brazilian fermented cassava food BJM
Crispim,S.M.; Nascimento,A.M.A.; Costa,P.S.; Moreira,J.L.S.; Nunes,A.C.; Nicoli,J.R.; Lima,F.L.; Mota,V.T.; Nardi,R.M.D..
Puba or carimã is a Brazilian staple food obtained by spontaneous submerged fermentation of cassava roots. A total of 116 lactobacilli and three cocci isolates from 20 commercial puba samples were recovered on de Man, Rogosa and Sharpe agar (MRS); they were characterized for their antagonistic activity against foodborne pathogens and identified taxonomically by classical and molecular methods. In all samples, lactic acid bacteria were recovered as the dominant microbiota (7.86 ± 0.41 log10 CFU/g). 16S-23S rRNA ARDRA pattern assigned 116 isolates to the Lactobacillus genus, represented by the species Lactobacillus fermentum (59 isolates), Lactobacillus delbrueckii (18 isolates), Lactobacillus casei (9 isolates), Lactobacillus reuteri (6 isolates),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Puba; Carimã; Fermented food; Lactobacillus spp.; Cassava; Antagonism.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100002
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Creole bean seeds microbiolization with doses of Trichoderma harzianum Ciência Rural
Dalzotto,Laís; Tortelli,Brenda; Stefanski,Francine Spitza; Sacon,Deivid; Silva,Vanessa Neumann; Milanesi,Paola Mendes.
ABSTRACT: In the search for improved yields, seed treatment by microbiolization has been used as an alternative to chemical treatment. The objective was to verify the physiological and sanitary quality of creole bean seeds, var. Chumbinho, after microbiolization with doses of a commercial product (c.p.) with Trichoderma harzianum (strain ESALQ-1306). The treatments were: T1) 100 mL c.p./100 kg seeds; T2) 150 mL c.p.; T3) 200 mL c.p.; T4) 200 mL of chemical treatment (c.p., 250 g L-1 fipronil + 25 g L-1 pyraclostrobin + 225 g L-1 thiophanate-methyl); and T5) control (without coating of seeds). The tests were: sanitary test (blotter test); germination and first count; accelerated aging, cold germination without soil, speed of germination rate (SGR), seedling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Biological control; Phaseolus vulgaris L.; Vigor..
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000500151
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Efecto de Trichoderma y Bacillus en la dinámica poblacional de Fusarium spp. en agave tequilero (Agave tequilana Weber. var. Azul) en Jalisco. Colegio de Postgraduados
Tlapal Bolaños, Bertha.
La marchitez es uno de los problemas fitosanitario más importantes en el agave tequilero Agave tequilana Weber var. Azul, enfermedad que esta asociada con varios microorganismos y que principalmente se presenta en plantaciones de más de 2 años de edad. Entre los posibles agentes causales de esta enfermedad se reportan a varias especies de Fusarium spp., así como a Thielaviopsis paradoxa. En el presente trabajo se planteó el de inocular con organismos benéficos plantas de agave tequilero que se usan como material propagativo en vivero para nuevas plantaciones, como parte de un manejo preventivo e integrado del cultivo. En este trabajo se estudió en vivero (Rancho El Indio, Tequila, Jalisco) y en campo, en plantaciones comerciales de agave en las...
Palavras-chave: Marchitez del agave; Fusarium spp.; Antagonismo; Supresividad; Agave wilt; Antagonism; Suppressive; Fitopatología; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/2117
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Rizobacterias y hongos micorrizógenos arbusculares como alternativa biotecnológica para mejorar el vigor y sanidad de portainjertos de cítricos. Colegio de Postgraduados
Chiquito Contreras, Roberto Gregorio.
El aprovechamiento de agentes biológicos como rizobacterias y hongos micorrizógenos arbusculares constituye una alternativa viable para disminuir el uso de agroquímicos en la producción agrícola. Se evaluó el potencial de rizobacterias y hongos micorrizógenos arbusculares como promotores del crecimiento y agentes de control biológico en plantas de cítricos. En la primera etapa de tres, se realizaron pruebas de reacción bioquímica, las cuales permitieron la identificación de las cepas FCA-56 y FCA-60 como Pseudomonas putida, FCA-8 como Pseudomonas fluorescens y en el caso de las cepas FCA-9, FCA-10, FCA-35 y FCA-52 se confirmó el género Pseudomonas. Además, se determinó la capacidad antagónica in vitro de las siete cepas rizobacterianas sobre el crecimiento...
Palavras-chave: Pseudomonas putida; Phytophthora parasitica; Antagonismo; ADNr16S; Colonizacion micorrízica; Antagonism; 16S rDNA; Mycorrhizal colonization; Agroecosistemas Tropicales; Doctorado.
Ano: 2011 URL: http://hdl.handle.net/10521/545
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Inoculación de bacterias promotoras de crecimiento vegetal en pepino (Cucumis sativus L.). Colegio de Postgraduados
Pérez Rosales, Elvia.
Las rizobacterias promotoras de crecimiento vegetal (RPCV) estimulan el crecimiento y rendimiento de algunos cultivos a través de mecanismos como: síntesis de fitohormonas, fijación de nitrógeno, solubilización de fosfatos, producción de sideróforos y control de fitopatógenos. El objetivo central de este trabajo fue evaluar la respuesta de pepino (Cucumis sativus L.) a la inoculación con RPCV. Para cumplir con este objetivo se realizaron cinco experimentos. El primero consistió en determinar el efecto de la inoculación de cuatro cepas de rizobacterias: Pseudomonas tolassi (P61), P. tolassi (A46), Bacillus pumilus (R44) y Microbacterium paraoxydans (P21) en el crecimiento de plántulas de pepino. El tratamiento donde se coinocularon las cepas A46+R44 tuvo la...
Palavras-chave: Trasplante; Hortalizas; Calidad de fruto; Antagonismo; Análisis económico-financiero; Transplanting; Vegetables; Fruit quality; Antagonism; Economic and financial analysis; Maestría; Edafología.
Ano: 2012 URL: http://hdl.handle.net/10521/729
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Rizobacterias y hongos micorrizógenos arbusculares como alternativa biotecnológica para mejorar el vigor y sanidad de portainjertos de cítricos. Colegio de Postgraduados
Chiquito Contreras, Roberto Gregorio.
El aprovechamiento de agentes biológicos como rizobacterias y hongos micorrizógenos arbusculares constituye una alternativa viable para disminuir el uso de agroquímicos en la producción agrícola. Se evaluó el potencial de rizobacterias y hongos micorrizógenos arbusculares como promotores del crecimiento y agentes de control biológico en plantas de cítricos. En la primera etapa de tres, se realizaron pruebas de reacción bioquímica, las cuales permitieron la identificación de las cepas FCA-56 y FCA-60 como Pseudomonas putida, FCA-8 como Pseudomonas fluorescens y en el caso de las cepas FCA-9, FCA-10, FCA-35 y FCA-52 se confirmó el género Pseudomonas. Además, se determinó la capacidad antagónica in vitro de las siete cepas rizobacterianas sobre el crecimiento...
Palavras-chave: Pseudomonas putida; Phytophthora parasitica; Antagonismo; ADNr16S; Colonizacion micorrízica; Antagonism; 16S rDNA; Mycorrhizal colonization; Agroecosistemas Tropicales; Doctorado.
Ano: 2011 URL: http://hdl.handle.net/10521/545
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Effects of culture filtrates of endophytic fungi obtained from Piper aduncum L. on the growth of Mycobacterium tuberculosis Electron. J. Biotechnol.
Lima,Alita Moura de; Salem,Júlia Ignez; Souza,João Vicente Braga de; Cortez,Ana Claudia Alves; Carvalho,Clarice Maia; Chaves,Francisco Célio Maia; Veiga Junior,Valdir Florêncio da.
Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from...
Tipo: Journal article Palavras-chave: Antagonism; Stimulation growth; Tuberculosis.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400008
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