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Assessment of the spatial variability of phenolic contents and associated bioactivities in the invasive alga Sargassum muticum sampled along its European range from Norway to Portugal ArchiMer
Tanniou, A.; Vandanjon, L.; Incera, M.; Serrano Leon, Esteban; Husa, V.; Le Grand, Jacqueline; Nicolas, Jean-louis; Poupart, N.; Kervarec, N.; Engelen, A.; Walsh, R.; Guerard, F.; Bourgougnon, N.; Stiger-pouvreau, Valerie.
Sargassum muticum, an invasive brown macroalga presently distributed along European Atlantic coasts from southern Portugal to the south coast of Norway, was studied on a large geographical scale for its production of phenolic compounds with potential industrial applications and their chemical and biological activities. S. muticum can produce high biomass in Europe, which could be exploited to supply such compounds. S. muticum was collected in Portugal, Spain, France, Ireland and Norway (three sites/country) to examine the effect of the latitudinal cline and related environmental factors. Assays focused particularly on polyphenols and their activities. Crude acetone–water extracts were purified using solid phase extraction (SPE) and antioxidant and...
Tipo: Text Palavras-chave: Antioxidant activities; Antibacterial activities; Latitudinal gradient; Phenolic compounds; Solid phase extraction; Sargassum muticum.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00171/28221/32175.pdf
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High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes ArchiMer
El Kheir, Sara M.; Cherrat, Lamia; Awussi, Ahoefa A.; Ramia, Nancy E.; Taha, Samir; Rahman, Abdur; Passerini, Delphine; Leroi, Francoise; Petit, Jeremy; Mangavel, Cecile; Revol-junelles, Anne-marie; Borges, Frederic.
This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of L. monocytogenes in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for...
Tipo: Text Palavras-chave: Biopreservation; Listeria monocytogenes; High-throughput screening assays; Bioluminescence; Competition; Mixed culture; Bioprotection; Antibacterial activities.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56534/58236.pdf
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An evaluation of antibacterial activities of Psidium guajava (L.) BABT
Sanches,Neviton Rogério; Garcia Cortez,Diógenes Aparício; Schiavini,Michelle Simone; Nakamura,Celso Vataru; Dias Filho,Benedito Prado.
The present study was designated to evaluate the antibacterial activities of aqueous and ethanol:water extracts from leaves, roots and stem bark of Psidium guajava L. The antibacterial activities of the extracts against bacteria were tested by using both microdilution assay. The aqueous extracts of P. guajava leaves, roots and stem bark were active against the gram-positive bacteria Staphylococcus aureus (MICs=500, 125 and 250 µg/ml, respectively) and Bacillus subtilis (MICs=500 µg/ml), and virtually inactive against the gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa (MICs >1000 µg/ml). The ethanol:water extracts showed higher antimicrobial activity as compared to aqueous extracts. Based on this finding, the ethanol:water extract of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Psidium guajava; Antibacterial activities; Flavonoids; Alpha- and beta-amyrin; Beta-sitosterol.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300014
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