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| Felipe,Rafaella Teles Arantes; Mourão Filho,Francisco de Assis Alves; Lopes,Silvio Aparecido; Mendes,Beatriz Madalena Januzzi; Behling,Maurel; Pereira Junior,Ernani Vicente. |
| The objective of this work was to evaluate the reaction of four sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' (Las) infection, a bacterium associated to huanglongbing (HLB) disease. Transgenic sweet orange plants of Hamlin, Natal, Pêra, and Valência cultivars, as well as nontransgenic controls received inocula by grafting budwood sections of HLB-infected branches. Disease progression was evaluated through observations of leaf symptoms and by polymerase chain reaction (PCR) analysis, eight months after inoculation. A completely randomized design was used, with four experiments (one for each cultivar) performed simultaneously. Bacteria title was estimated by quantitative PCR (qPCR). HLB symptoms and Las titers... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus sinensis; Antibacterial peptide; Disease resistance; Genetic transformation; Huanglongbing. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013001100004 |
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