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| Registros recuperados: 29 | |
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| Dixon,Brian. |
| Aquaculture still faces serious economic impacts due to the loss of animals to disease. A conservative estimate of 5% losses due to disease means that the finfish aquaculture industry loses over $1 billion annually on a global scale. One proven way to prevent costly disease outbreaks is to vaccinate fish against common or known pathogens. Current vaccination schemes still result in losses, however, and this may be due in part to vaccine design. Vaccines are currently designed using state of the art knowledge of immune responses, which is based primarily on mammalian studies. Just how applicable is this information to fish immunity and vaccine design, however? This review discusses what is currently known for teleost fish about two key processes that drive... |
| Tipo: Journal article |
Palavras-chave: Antibody; Antigen presentation; Cloning; Cytokine; Immunity; Major histocompatibility complex receptors. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500014 |
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| Olabuenaga,Susana E.. |
| The defence against infections is the essential function of the immune system of all Vertebrates. Fish present an efficient and developed immunologic response and in the case of Teleosts, with some similarities to the higher vertebrates. It can be divided into two types: innate or non specific and adquired or specific, both with a humoral and cellular compartments. Their relative importance can change according to the age of fish and is always under the influence of different factors. The most relevant components are mentioned here. There are two factors in fishes which strongly influence the immune response and antibody production, seasonal changes and temperature. The main lymphoid organs are described here: the thymus, the kidney and the spleen, as well... |
| Tipo: Journal article |
Palavras-chave: Antigen; Antibody; Leukocytes; Phagocytosis; Lymphokines; Inflammation; Immunostimulants. |
| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382000000200010 |
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| Zhao,Hai-Xia; Li,Xin-Yu; Guan,Wen-Ying; Han,Xiao-Tong. |
| Abstract The aim of this study was to evaluate the impact and mechanism of co-blocking of costimulatory signals CD28-B7-CD40-CD40L during immune allograft rejection. Forty-eight recipient rabbits were prepared as a high-risk corneal allograft model. After surgery, the animals were randomly divided into: control group, MR1 group, anti-B7 group, and co-blocking group (n=12, each group). Subconjunctival injection was first performed on the allograft surgery day until post-surgery day five. Four weeks later, or when immune rejection occurred, the cornea was sampled to detect and analyze the gene spectrum. The survival time in the co-blocking group was significantly longer than that in the other three groups (p < 0.05). Gene expression analysis revealed that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Costimulatory signal; Corneal allograft; Immune rejection; Second-generation sequencing technology; Antibody. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300472 |
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| Briand, Elisabeth; Salmain, Michèle; Compere, Chantal; Pradier, Claire-marie. |
| Binary mixtures of 11-mercaptoundecanoic acid (MUA) and other thiols of various lengths and terminal functions were chemisorbed on gold-coated surfaces via S-Au bonds to form mixed self-assembled monolayers (SAMs). Several values of the inole fraction of MUA in the thiol mixtures were tested and the structure and composition of the resulted thin films were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results made it clear that co-adsorption of MUA with thiols of similar chain length led to well-ordered monolayers whereas the co-adsorption of MUA with shorter thiols yielded less crystalline-like thin films, but with more reactive carboxylic acid terminal... |
| Tipo: Text |
Palavras-chave: PM IRRAS; Antibody; Protein A; Gold; Self assembled monolayers. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2222.pdf |
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| Gas, Fabienne; Baus, Beatrice; Pinto, Laetitia; Compere, Chantal; Tanchou, Valerie; Quemeneur, Eric. |
| Harmful algal blooms represent a major threat to marine production, and particularly to shellfish farming. Current methods for analyzing environmental samples are tedious and time consuming because they require taxonomists and animal experiments. New rapid detection methods, such as immunoassays, are sought for alerting purposes and for the study of algal ecodynamics in their natural environment. Alexandrium minutum, which causes paralytic shellfish poisoning, occurs with increasing frequency along European coasts. We have developed a one step immunochromatographic assay which is based on the principle of immunochromatographic analysis and involves the use of two distinct monoclonal antibodies directed against surface antigens of A. minutum. The primary... |
| Tipo: Text |
Palavras-chave: Antibody; Alexandrium minutum; Immunochromatographic assay (ICA); Rapid detection. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00000/11153/7819.pdf |
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| Coussot, Gaelle; Le Postollec, Aurelie; Faye, Clement; Baque, Mickael; Vandenabeele-trambouze, Odile; Incerti, Sebastien; Vigier, Flavie; Chaput, Didier; Cottin, Herve; Przybyla, Bartos; Berger, Thomas; Dobrijevic, Michel. |
| Antibody-based analytical instruments are under development to detect signatures of life on planetary bodies. Antibodies are molecular recognition reagents able to detect their target at sub-nanomolar concentrations, with high affinity and specificity. Studying antibody binding performances under space conditions is mandatory to convince space agencies of the adequacy of this promising tool for planetary exploration. To complement previous ground-based experiments on antibody resistance to simulated irradiation, we evaluate in this paper the effects of antibody exposure to real space conditions during the EXPOSE-R2 mission outside the International Space Station. The absorbed dose of ionizing radiation recorded during the 588 days of this mission (220 mGy)... |
| Tipo: Text |
Palavras-chave: Astrobiology; Cosmic rays; Biochip; Antibody; Planetary exploration. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00601/71305/69721.pdf |
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| Albas,A.; Fontolan,O. L.; Pardo,P. E.; Bremer Neto,H.; Sartori,A.. |
| In this study, we compared the levels of neutralizing antibodies induced by inactivated rabies vaccine in cattle by using three alternative immunization procedures. Forty-five bovines (breed nelore) were then organized in three groups (A, B and C, with 15 animals/group). Group A received only one vaccine dose at day zero and Group B received the first dose at day zero and then another dose at day 30 (early booster). Group C was also immunized with two doses; however, the booster was postponed until day 180 after the first dose (delayed booster). Blood samples were withdrawn at days zero (before the first dose) and 30, 210, 390, and 540 after the beginning of immunization and the antibody titers were evaluated by mouse neutralization test. The protocol used... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabies; Vaccine; Cattle; Antibody; Neutralization. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300010 |
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| Rzepczyk, C. M.; Anderson, K. L.; Crurshes, P. A.; Baxter, E. P.; Kere, N.; Irving, D.; Dyer, S.; Jones, G. L.. |
| Merozoite surface antigen 2 (MSA2) is a Plasmodium falciparum vaccine candidate. Antibody responses to MSA2 in Melanesians naturally exposed to P. falciparum were investigated by ELISA using the recombinant MSA2 protein known as Ag 1609 and 65 overlapping synthetic peptides spanning predominantly the conserved N and C terminal regions of MSA2. Significant differences were obtained between control and malaria exposed subjects in the ability of sera to bind to Ag 1609. The median absorbance obtained with control sera was 0.36 whereas that obtained with sera from malaria exposed subjects was 1.8. The sera of ninety-five percent of malaria exposed subjects reacted significantly with Ag 1609. The percentage reactivity of the control sera with Ag 1609 was 39%.... |
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Palavras-chave: Malaria; Merozoite surface antigen 2 (MSA2); Antibody. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/170 |
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| da Silva,ICM; Ribeiro,AML; Canal,CW; Trevizan,L; Macagnan,M; Gonçalves,TA; Hlavac,NRC; de Almeida,LL; Pereira,RA. |
| One to 42-d-old (432) female broilers were fed different levels of inorganic selenium (ISe) and organic selenium (OSe), according to the following treatments: (1) 0.3 mg ISe; (2) 0.3 mg ISe + 0.2 mg OSe; (3) 0.5 mg ISe and (4) 0.3 mg OSe/ kg of feed. All birds were vaccinated against infectious bursal disease (IBD) at 19d of age and three birds/replicate (R) were inoculated with sheep red blood cells (SRBC) at 32d. Three other birds/R received Freund's adjuvant at 37d and avian tuberculin (AT) in the wattle at 47d of age. All birds were submitted to heat stress after 21d. Performance parameters, bursa and spleen weights, lymphocyte bursa depletion, antibody (Ab) production against IBD and SRBC, hematocrit, leukocytes, heterophil/lymphocyte ratio (H/L), and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibody; Broiler; Infectious bursal disease; Performance; Selenium. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000400005 |
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| Rauber,RH; Flôres,ML; Pereira,CE; Grigulo,M. |
| The present work was carried out to compare Infectious Bronchitis Virus (IBV) antibody titers in serum and egg yolk samples from laying hens. Sixty paired blood and egg samples were collected from laying hens of two farms. Serum samples were frozen, while egg yolk samples were diluted (1:500) before freezing. Serum and yolk samples were tested for the presence of IBV antibodies by indirect ELISA (commercial kit) and titers were compared by a correlation test (alpha=0.05). There was a high correlation (r=0.62) between the two kinds of samples, which means that titers of IBV antibodies in the egg yolk and in serum samples are quite the same. Considering that blood collection causes deep stress that leads to economic losses, and since eggs are collected daily... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibody; Egg yolk; ELISA; Infectious bronchitis; Serology. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200008 |
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| PAVAN,TATIANA R.; NIED,CRISTIAN O.; NORO,MIRELA; ANZILIERO,DENIZ; FRANDOLOSO,RAFAEL; KREUTZ,LUIZ CARLOS. |
| ABSTRACT Molecules with immune modulating activity are ubiquitously distributed in nature and their impact on aquaculture has been exploited in order to increase fish resistance to pathogens. Here, we investigated the effect of inactivated Parapoxvirus ovis (iPPVO) on blood cells and innate and acquired immune response of silver catfish (Rhamdia quelen). iPPVO inoculation had no effect on respiratory burst activity; however, following iPPVO inoculation, we observed a significant decrease on circulating monocytes concomitantly with an increased number of heterophilic granulocytes and thrombocytes, which are the main cells involved in innate immunity and provide connection with acquired immunity. Fish inoculated with a combination of bovine serum albumin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunostimulant; Adjuvant; Antibody; Fish. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401451 |
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| Kokron,Cristina M.; Errante,Paolo R.; Barros,Myrthes T.; Baracho,Gisele V.; Camargo,Maristela M.; Kalil,Jorge; Rizzo,Luiz V.. |
| Common variable immunodeficiency (CVID) is an immunological disorder characterized by defective antibody production, recurrent infections, most notably of the respiratory tract, autoimmune phenomena and cancer. Some CVID patients may also present disturbances of the cellular immune response such as a decrease in the number and proportion of different lymphocyte populations, diminished lymphoproliferative response to mitogens and antigens, altered production of cytokines, and deficient expression of cell-surface molecules. Most Brazilian CVID patients included in this study show a decrease in T and B lymphocyte counts in the peripheral blood. Furthermore, their lymphocytes are more susceptible to apoptosis following activation than normal individuals, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunodeficiency; Humans; Antibody; Cytokines; Clinical features. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000400007 |
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| Cardoso,W.M.; Gomes,L.P.; Romão,J.M.; Salles,R.P.R.; Teixeira,R.S.C.; Sobral,M.H.N.R.; Câmara,S.R.; Oliveira,W.F.. |
| The experiment was carried out to determine the antibody levels to infectious bronchitis virus (IBV) in 1120 broilers of two broiler flocks, both from the same parental flock and free from previous vaccination. Forty chicks of each line were alloted to the control group and the sera were tested by indirect ELISA. The vaccination program consisted on the administration of commercial vaccines against IBV at 10 and 25 days of age. Chicks with low levels of maternal antibodies (Mab) did not show significant titers to the first vaccinal stimulus. They presented a vaccinal response to the second vaccinal stimulus reaching the top around GMT 1100 at 45 days. Chicks with high Mab titers did not show significant titers to the primary and secondary vaccinal stimuli,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler; Infections bronchitis; Antibody. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300007 |
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| Vestri,Sandro; Nikami,Hideki; Saito,Masayuki; Machado,Ubiratan F.. |
| In the present study, the production of an anti-GLUT 2 antibody is reported. The antibody (S-5096) was raised in New Zealand rabbits immunized with an immunogen prepared by cross-linking the synthetic peptide and a carrier protein. A COOH-terminal decapeptide of rat GLUT 2 predicted sequence was synthesized and coupled to keyhole limpet hemocyanin, using the glutaraldehyde method. Subcellular membrane fractions were prepared from renal cortex (C) and medulla (M), and subjected to Western blotting analysis using the antiserum in a 1:200 dilution and, subsequently, [125I]protein A. As the results showed, the S-5096 anti-serum showed clear blots in kidney samples, stronger in cortex than in medulla as expected, whereas white adipose tissue and heart samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glucose transporter; GLUT 2; Antibody; Coupling peptide. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200004 |
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| Registros recuperados: 29 | |
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