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The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein Electron. J. Biotechnol.
Wang,Mengqiang; Zhang,Daoxiang; Wang,Lingling; Gai,Yunchao; Zhou,Zhi; Zhang,Huan; Song,Linsheng.
Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities. Results: The full-length cDNA of crab Eriocheir sinensis CypA (EsCypA) was cloned by EST and RACE technique. The complete sequence of EsCypA cDNA contained a 5' untranslated region (UTR) of 50 bp, a 3’ UTR of 233 bp with a polyA tail, and an open reading frame (ORF) of 495 bp encoding a polypeptide of 164 amino acids with the predicted molecular weight of 17.36 kDa. The deduced amino acid sequence of EsCypA contained two highly conserved signature sequences of...
Tipo: Journal article Palavras-chave: Antifungal activity; Cyclophilin A; Eriocheir sinensis; Immune response; PPIase activity.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200002
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Characterization of the antifungal activity of Lactobacillus harbinensis K.V9.3.1Np and Lactobacillus rhamnosus K.C8.3.1I in yogurt ArchiMer
Delavenne, Emilie; Cliquet, Sophie; Trunet, Clément; Barbier, Georges; Mounier, Jérome; Le Blay, Gwenaelle.
Few antifungal protective cultures adapted to fermented dairy products are commercially available because of the numerous constraints linked to their market implementation. Consumer’s demand for naturally preserved food products is growing and the utilization of lactic acid bacteria is a promising way to achieve this goal. In this study, using a 25-1 factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contamination amount of a spoilage yeast, on antifungal activities of single and mixed cultures of Lactobacillus rhamnosus K.C8.3.1I and Lactobacillus harbinensis K.V9.3.1Np in yogurt. L. harbinensis K.V9.3.1Np, the most relevant strain with regard to antifungal activity was...
Tipo: Text Palavras-chave: Lactobacillus harbinensis; Bioprotective culture; Antifungal activity.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00189/30025/28510.pdf
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Antifungal Activity of Aerial Parts of Cenchrus pennisetiformis Against Fusarium oxysporum f. sp. lycopersici Planta Daninha
KHURSHID,S.; JAVAID,A.; SHOAIB,A.; JAVED,S.; QAISAR,U..
ABSTRACT: Antifungal potential of aerial parts of an allelopathic grass Cenchrus pennisetiformis (Hochst. & Steud.) Wipff. was evaluated against Fusarium oxysporum f. sp. lycopersici Snyder & Hansen, a fungal pathogen causing wilt disease in tomato (Solanum lycopersicum L.). Different concentrations (1% to 6%) of methanolic leaf, stem and inflorescence extract of the grass significantly reduced fungal biomass by 40-88%, 13-89%, and 26-76%, respectively. Methanolic shoot (leaf + stem) extract was fractionated using four organic solvents viz. n-hexane, chloroform, ethyl acetate and n-butanol. All the sub-fractions of methanolic shoot extract showed remarkable antifungal potential to variable extents. Different concentrations (1.56-200 mg mL-1) of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal activity; GC-MS analysis; Methanolic extracts.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100223
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ANTIFUNGAL POTENTIAL OF A BRASSICACEOUS WEED Sisymbrium irio AGAINST Macrophomina phaseolina Planta Daninha
JAVAID,A.; AFZAL,L.; SHOAIB,A..
ABSTRACT Macrophomina phaseolina, a soil-borne plant pathogen, has the ability to cause diseases in about 500 plant species. Unfortunately, so far no registered fungicide is available against this fungal pathogen. In the present study, different extracts of Sisymbrium irio, a weed of family Brassicaceae, were tested for evaluation of their antifungal activity against M. phaseolina. In screening bioassays, antifungal activity of methanolic extracts of 1% to 6% concentrations of different parts (leaf, stem, root and fruit) of the weed was assessed against the fungal pathogen. Methanolic leaf and root extracts significantly reduced fungal biomass up to 59% and 69% over control, respectively. Methanolic leaf and root extracts were further partitioned using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal activity; London rocket; Plant extracts.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100255
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ANTIFUNGAL ACTIVITY OF Cirsium arvense EXTRACTS AGAINST PHYTOPATHOGENIC FUNGUS Macrophomina phaseolina Planta Daninha
BANARAS,S.; JAVAID,A.; SHOAIB,A.; AHMED,E..
ABSTRACT Leaves, stems, roots and inflorescence of the asteraceous weed Circium arvense were extracted in methanol for two weeks. Methanol was evaporated in a rotary evaporator. Different concentrations (1, 2, 3, 4 and 5%) of methanolic extracts were prepared, and their antifungal activities were studied against Macrophomina phaseolina, using malt extract broth as growth medium. In general, extracts of all plant parts showed antifungal activities to variable extents. The highest antifungal activity occurred due to methanolic extract of leaves, followed by stem and root extracts, resulting in 10-74%, 6-57% and 11-39% reduction in fungal biomass over control, respectively. Inflorescence extract showed the least antifungal activity, resulting in 2-30%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal activity; Asteraceous weed; Cirsium arvense; Macrophomina phaseolina.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100216
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Bioassays Guided Fractionation of Senna occidentalis for Identification of Natural Antifungal Constituents Against Macrophomina phaseolina Planta Daninha
JAVAID,A.; QUDSIA,H.; SHOAIB,A..
ABSTRACT Macrophomina phaseolina is one of the most destructive soil-borne fungal plant pathogens. In the present study, different aerial parts of Senna occidentalis were analyzed for their antifungal activity against M. phaseolina. In initial screening bioassays, 0.5-3.0% concentrations of methanolic extracts of fruit, leaf and stem were tested against M. phaseolina. The methanolic leaf extract showed the greatest activity causing up to 29% suppression in biomass production of M. phaseolina. Fractionation of leaf extract of S. occidentalis was carried out with four organic solvents. Bioassays with a range of concentrations (2.34-150 mg mL-1) of these sub-fractions revealed that the chloroform sub-fraction was the most effective, causing 93-98% reduction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal activity; Leaf extract; Macrophomina phaseolina; Natural fungicide; Senna occidentalis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100210
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In vitro effect of essential oils on Alternaria solani Sorauer Rev. Protección Veg.
Duarte,Yanisia; Pino,Oriela; Infante,Danay; Sánchez,Yaima; Travieso,María del Carmen; Martínez,B.
The aim of this work was to determine the in vitro antifungal activity of ten essential oils on Alternaria solani SORAUER. The effect by direct contact and exposure to vapors was evaluated. The bioassays were performed using a completely randomized design and the method of paper discs inoculated with the oils facing phytopathogen discs; the fungal radial growth was evaluated. All the oils, except those from Citrus sinensis (L.) Osbeck (sweet orange), inhibited the mycelial growth until the seventh day. At day 14, a total inhibition was observed in the treatments with the oils from Pimpinella anisum L. (anise), Ocimum basilicum L. (white basil), Ocinum basilicum L. var. genovese (Genovese basil), and Piper auritum Kunth (anise caisimon). No fungicidal...
Tipo: Journal article Palavras-chave: Essential oils; Antifungal activity; Ocimum basilicum; Piper marginatum; Ruta chalepensis; Pimpinella anisum; Piper auritum; Piper aduncum subsp. Ossanum; Citrus sinensis.
Ano: 2013 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522013000100007
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Identification of the major fungitoxic component of cinnamon bark oil Trop. Plant Pathol.
Jham,Gulab N.; Dhingra,Onkar D.; Jardim,Carolina M.; Valente,Vânia M. M..
The study was done to identify the most active fungitoxic component of cinnamon bark (Cinnamomum zeylanicum) oil that can be used as a marker for standardization of cinnamon extract or oil based natural preservative of stored seeds. Aspergillus flavus and A. ruber were used as test fungi. The hexane extracted crude oil and the hydro-distilled essential oil from cinnamon bark had complete growth inhibition concentration (CGIC) of 300 and 100 µl/l, respectively. Both oils produced three fractions on preparative thin layer silica-gel chromatography plates. The fraction-2 of either oil was the largest and most active, with CGIC of 200 µl/l, but the fungitoxicity was also retained in the other two fractions. The fraction-1 and 3 of the crude oil reduced growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oils; Antifungal activity; Fungitoxicity; Active compounds; Cinnamon oil; Seed preservative.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400011
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Antifungal activity of selected plant extracts based on an ethnodirected study Acta Botanica
Silva,Flávia dos Santos; Landell,Melissa Fontes; Paulino,Gustavo Vasconcelos Bastos; Coutinho,Henrique Douglas Melo; Albuquerque,Ulysses Paulino.
ABSTRACT Plants have been reported as used by local populations to treat various infections for a long time, which has directed several pharmacological studies. The main aim of this work was to evaluate three plant selection criteria with better predictive power to detect extracts with antifungal action: (1) medicinal plants that are not used for indications of infection and inflammation; (2) plants with direct citations for inflammation, except for infection; (3) plants with direct citations for inflammation and infection selected quantitatively by Syndromic Importance Value (SIV). We tested the action of 23 hydroethanolic extracts of plants against the fungi Candida albicans, Cryptococcus neoformans, and Cryptococcus gattii and found no differences in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Antifungal activity; Ethnobotany; Ethnopharmacology; Natural products; Local medical systems.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000200442
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Antifungal activity of the extracts and saponins from Sapindus saponaria L. Anais da ABC (AABC)
Tsuzuki,Joyce K.; Svidzinski,Terezinha I.E.; Shinobu,Cristiane S.; Silva,Luiz F.A.; Rodrigues-Filho,Edson; Cortez,Diógenes A.G.; Ferreira,Izabel C.P..
Extracts from the dried pericarp of Sapindus saponaria L. (Sapindaceae) fruits were investigated for their antifungal activity against clinical isolates of yeasts Candida albicans and C. non-albicans from vaginal secretions of women with Vulvovaginal Candidiasis. Four clinical isolates of C. albicans, a single clinical isolated of each of the species C. parapsilosis, C. glabrata, C. tropicalis, and the strain of C. albicans ATCC 90028 were used. The hydroalcoholic extract was bioactivity-directed against a clinical isolate of C. parapsilosis, and showed strong activity. The n-BuOH extract and one fraction showed strong activity against all isolates tested. Further column-chromatography on silica gel separation of this fraction afforded two pure triterpene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sapindaceae; Sapindus saponaria; Saponins; Antifungal activity; Candida.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000400002
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Complete ¹H and 13C NMR assignments and anti fungal activity of two 8-hydroxy flavonoids in mixture Anais da ABC (AABC)
Johann,Susana; Smânia-Jr,Artur; Pizzolatti,Moacir G.; Schripsema,Jan; Braz-Filho,Raimundo; Branco,Alexsandro.
A mixture of the two new flavonols 8-hydroxy-3, 4', 5, 6, 7-pentamethoxyflavone (1) and 8-hydroxy-3, 3', 4', 5, 6, 7-hexamethoxyflavone (2) was isolated from a commercial sample of Citrus aurantifolia. An array of one- (¹HNMR, {¹H}-13C NMR, and APT-13C NMR) and two-dimensional NMR techniques (COSY, NOESY, HMQC and HMBC) was used to achieve the structural elucidation and the complete ¹H and 13C chemical shift assignments of these natural compounds. In addition, the antifungal activity of these compounds against phytopathogenic and human pathogenic fungi was investigated.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymethoxyflavones; Antifungal activity; Citrus aurantifolia.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000200004
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Essential oils from Piper aduncum inflorescences and leaves: chemical composition and antifungal activity against Sclerotinia sclerotiorum Anais da ABC (AABC)
VALADARES,ANNA CAROLINA F.; ALVES,CASSIA CRISTINA F.; ALVES,JOSÉ MILTON; DE DEUS,ISABELLA P.B.; DE OLIVEIRA FILHO,JOSEMAR G.; DOS SANTOS,TAINÁ CAROLINE L.; DIAS,HERBERT JÚNIOR; CROTTI,ANTÔNIO EDUARDO M.; MIRANDA,MAYKER L.D..
Abstract Even though essential oils from Piper aduncum (Piperaceae) have different biological activities, little is known about their application to agricultural areas. White mold is a plant disease caused by the phytopathogen Sclerotinia sclerotiorum, which needs to be controlled by alternative measures. This study aimed at evaluating the effect of essential oils from P. aduncum leaves (PL-EO) and inflorescences (PI-EO) on the mycelial growth of S. sclerotiorum. Essential oils from P. aduncum were obtained by hydrodistillation by a Clevenger-type apparatus while their chemical composition was analyzed by GC-MS and GC-FID. Piperitone (23.4 %), myristicin (12.4 %), terpinen-4-ol (12.3 %), β-caryophyllene (7.2 %), α-humulene (6.9 %), germacrene-D (6.9 %) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piper aduncum; Essential oils; Piperitone; Antifungal activity; Sclerotinia sclerotiorum.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602691
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Screening of Brazilian plants for antimicrobial and dnadamaging activities: I. Atlantic rain forest . Ecological station juréia-itatins Biota Neotropica
Agripino,Débora Gomes; Lima,Marcos Enoque Leite; Silva,Marcelo Rogério da; Meda,Cristiane Infante; Bolzani,Vanderlan da Silva; Cordeiro,Inês; Young,Maria Claudia Marx; Moreno,Paulo Roberto H..
I. Atlantic Rain Forest . Ecological Station Juréia-Itatins) Crude extracts from 88 plant species occurring in São Paulo State (Brazil), collected in an Atlantic Forest region, have been screened for antimicrobial and DNA-damaging activities. Of the 114 extracts assayed for antibacterial activity, only the extracts from leaves and stems of Aspidosperma ramiflorum (Apocynaceae) showed a slight activity against Escherichia coli. In the antifungal assay with Candida albicans, no active extract was observed, while in the bioautography assay with Cladosporium sphaerospermum and C. cladosporioides 12% were active. However, only the extract of Psychotria mapoureoides (Rubiaceae) stems showed a strong inhibition of both fungi. The DNA-damaging assay with mutant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atlantic Rain forest; Antibacterial activity; Antifungal activity; Antitumoral activity; Plants screening.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032004000200009
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Antifungal activity of essential oil isolated from Ocimum gratissimum L. (eugenol chemotype) against phytopathogenic fungi BABT
Faria,Terezinha de Jesus; Ferreira,Rafael Sottero; Yassumoto,Lidiane; Souza,José Roberto Pinto de; Ishikawa,Noemia Kazue; Barbosa,Aneli de Melo.
An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ocimum gratissimum; Antifungal activity; Eugenol; Phytopathogenic fungi.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700002
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Alternative Control of Plant Pathogen Fungi Through Ethanolic Extracts of Avocado Seeds (Persea Americana Mill.) BABT
Fagundes,Miriã Cristina Pereira; Oliveira,Adelson Francisco de; Carvalho,Vicente Luiz de; Ramos,José Darlan; Santos,Verônica Andrade dos; Rufini,José Carlos Moraes.
ABSTRACT The aim of this study was to evaluate the antifungal action of ethanolic extracts of avocado seeds, by conducting two experiments in the laboratory of plant pathology of EPAMIG SUL/Lavras, MG, Brazil, in a completely randomized design with three replicates. The first consisted of the following treatments ('Breda' 3%, 'Breda' 2%, 'Margarida' 3%, 'Margarida' 2%, Control, and Ethanol) testing its inhibitory effect on two fungal species (Colletotrichum gloeosporioides and Monilinia fructicola) and the second consisted of different concentrations of extracts (0%, 0.25%, 0.50%, 0.75%, 1.0%, 1.5%, and 2.0%) on the fungus F fructicola. The evaluations were performed at three times (7, 14, and 21 days of incubation) by measurements of the mycelial diameter...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal activity; Colletotrichum gloeosporioides; Monilinia fructicola; Postharvest.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100204
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Antimicrobial effectiveness of spices: an approach for use in food conservation systems BABT
Souza,Evandro Leite de; Stamford,Tânia Lúcia Montenegro; Lima,Edeltrudes de Oliveira; Trajano,Vinícius Nogueira; Barbosa Filho,José Maria.
There has been constant an increasing the search alternative and efficient compounds for food conservation, aiming a partial or total replacement of antimicrobial chemical additives. Spices offer a promising alternative for food safety. Inhibitory activity of spices and derivatives on the growth of bacteria, yeasts, fungi and microbial toxins synthesis has been well reported, so they could be used in food conservation as main or as adjuvant antimicrobial compounds in order to assure the production of microbiologically stable foods.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spices; Essential oil; Extracts; Chemical compounds; Antibacterial activity; Antifungal activity.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500007
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GC-MS analysis of Nigella sativa seeds and antimicrobial activity of its volatile oil BABT
Gerige,Saptha Jyothi; Gerige,Mahesh Kumar Yadav; Rao,Muralidhara; Ramanjaneyulu,.
The present study dealt with the hydro distillation of Nigella sativa seeds and GC-MS analysis. The total composition of the oil was 86.7%. The seed volatile oil was tested against 19 microbes (Gram positive, Gram negative and fungi), which showed that Gram negative bacteria Haemophilus influenza, Klebsiella pnemoniae, and Proteus vulgaris were highly sensitive against the antimicrobial agent, whereas the fungi such as Trichoderma vibriae,. Pencillium rubrum; and Dermatophyte: Trichophyton mentagrophytes; had no response at the 20µl concentration.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nigella sativa; Volatile oil; Antimicrobial activity; Antifungal activity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500016
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Changes in hyphal morphology due to chitosan treatment in some fungal species BABT
Oliveira Junior,Enio Nazaré de; Melo,Itamar Soares de; Franco,Telma Teixeira.
In this work, changes in the hyphal morphology due to chitosan treatment in some fungal species were studied. Scanning electron microscope (SEM) observations revealed that chitosans with molar fraction of acetyl groups (F A 0.16 and 0.18) and degree of polymerization (DP 1,089 and 1,242) had a direct effect on the morphology of the chitosan-treated fungi, reflecting its potential for causing a delay in the growth of Alternaria alternata (500 µg × mL-1), Botrytis cinerea (1,000 µg × mL-1), Penicillium expansum (1,000 µg × mL-1) and Rhizopus stolonifer (500 µg × mL-1). Mycelial aggregation and structural changes such as excessive branching, swelling of the cell wall and hyphae size reduction were observed in the micrographs.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosan; Alternative control of fungi; Antifungal activity; Plant pathogenic fungi; Fungal morphology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500001
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Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies BJID
Brilhante,Raimunda Sâmia Nogueira; Caetano,Erica Pacheco; Oliveira,Jonathas Sales de; Castelo-Branco,Débora de Souza Collares Maia; Souza,Elizabeth Ribeiro Yokobatake; Alencar,Lucas Pereira de; Cordeiro,Rossana de Aguiar; Bandeira,Tereza de Jesus Pinheiro Gomes; Sidrim,José Júlio Costa; Rocha,Marcos Fábio Gadelha.
ABSTRACTThe antifungal activity of some statins against different fungal species has been reported. Thus, at the first moment, the in vitro antifungal activity of simvastatin, atorvastatin and pravastatin was tested againstCandida spp. and Cryptococcus spp. Then, in a second approach, considering that the best results were obtained for simvastatin, this drug was evaluated in combination with antifungal drugs against planktonic growth and tested against biofilms ofCandida spp. and Cryptococcus spp. Drug susceptibility testing was performed using the microdilution broth method, as described by the Clinical and Laboratory Standards Institute. The interaction between simvastatin and antifungals against planktonic cells was analyzed by calculating the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida; Cryptococcus; Simvastatin; Antifungal activity; Biofilm.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500459
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Antifungal treatment with carvacrol and eugenol of oral candidiasis in immunosuppressed rats BJID
Chami,N.; Chami,F.; Bennis,S.; Trouillas,J.; Remmal,A..
Carvacrol and eugenol, the main (phenolic) components of essential oils of some aromatic plants, were evaluated for their therapeutic efficacy in the treatment of experimental oral candidiasis induced by Candida albicans in immunosuppressed rats. This anticandidal activity was analyzed by microbiological and histopathological techniques, and it was compared with that of nystatin, which was used as a positive control. Microbiologically, carvacrol and eugenol significantly (p<0.05) reduced the number of colony forming units (CFU) sampled from the oral cavity of rats treated for eight consecutive days, compared to untreated control rats. Treatment with nystatin gave similar results. Histologically, the untreated control animals showed numerous hyphae on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carvacrol; Eugenol; Experimental oral candidiasis; Antifungal activity; Essential oils.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000300005
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