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| Peter,C.M.; Waller,S.B.; Picoli,T.; Osório,L.G.; Zani,J.L.; Meireles,M.C.A.; Faria,R.O.; Mello,J.R.B.; Hubner,S.O.; Lima,M.; Fischer,G.. |
| ABSTRACT In this study, we described the antifungal activity of three Brazilian propolis extracts: brown, green and from jataí bees against Sporothrix brasiliensis. The extracts were obtained from ethanolic extraction and their chemical composition was determined by high-performance liquid chromatography coupled to mass spectrometry. The cellular toxicity was measured in MDBK (Madin-Darby Bovine Kidney) cells and quantified by the MTT assay (3- (4,5 dimethylthiazol-2yl -2,5-diphenyl-2H bromine tetrazolato). For antifungal activity, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined by broth microdilution. The results showed that cell toxicity was not observed at lower concentrations (0.097 to 0.39μg/ml)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zoonosis; Itraconazole; Antifungal resistance; Therapeutic alternative. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300819 |
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| Waller,S.B.; Madrid,I.M.; Cleff,M.B.; Santin,R.; Freitag,R.A.; Meireles,M.C.A.; Mello,J.R.B.. |
| ABSTRACT Rosmarinus officinalis L. (rosemary) and Origanum vulgare L. (oregano) are known to have antimicrobial properties, but studies on sporotrichosis are scarce. This study aimed to evaluate the anti-Sporothrix spp. activity of essential oils from commercial products and oils extracted from aerial parts of these plants and analyze their chemical constituents. S. schenckii complex and S. brasiliensis (n: 25) isolated from humans, cats, dogs, and environmental soil were tested through M27-A3 guidelines of CLSI with modification for phytotherapics. The essential oils of R. officinalis L. were similar for MIC50 and MFC50 ≤2.25mg/mL for extracted oil; and 4.5mg/mL and 9mg/mL, respectively, for commercial oil. Both products showed MIC90 of 18mg/mL and MFC90... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporotrichosis; Antifungal resistance; Oregano; Rosemary; Lamiaceae. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400991 |
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| Colombo,Arnaldo L.; Matta,Daniel da; Almeida,Leila Paula de; Rosas,Robert. |
| The increasing magnitude of antifungal resistance as well as the advent of new antifungal drugs has generated a renewed interest in fungal susceptibility testing. We used a previously described disk diffusion method to evaluate the susceptibility profile of a large collection of recent clinical Candida spp. isolates against fluconazole. A total of 1,784 yeast isolates were tested, including the following species: Candida albicans (1,036), C. tropicalis (279), C. parapsilosis (202), C. glabrata (119), C. guilliermondii (90), C. krusei (32), C. lusitaniae (7), Candida spp. (14) and other yeasts (5). Susceptibility ranking to fluconazole obtained with all yeasts tested was: C. parapsilosis <FONT FACE=Symbol>@</FONT> C. tropicalis <FONT... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida; Fluconazole; Antifungal resistance; Disk diffusion method. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000300003 |
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| Brilhante,Raimunda S.N.; Paiva,Manoel A.N.; Sampaio,Célia M.S.; Castelo-Branco,Débora S.C.M.; Teixeira,Carlos E.C.; Alencar,Lucas P. de; Bandeira,Tereza J.P.G.; Monteiro,André J.; Cordeiro,Rossana A.; Pereira-Neto,Waldemiro A.; Sidrim,José J.C.; Moreira,José L.B.; Rocha,Marcos F.G.. |
| Abstract Since, there is no study reporting the mechanism of azole resistance among yeasts isolated from aquatic environments; the present study aims to investigate the occurrence of antifungal resistance among yeasts isolated from an aquatic environment, and assess the efflux-pump activity of the azole-resistant strains to better understand the mechanism of resistance for this group of drugs. For this purpose, monthly water and sediment samples were collected from Catú Lake, Ceará, Brazil, from March 2011 to February 2012. The obtained yeasts were identified based on morphological and biochemical characteristics. Of the 46 isolates, 37 were Candida spp., 4 were Trichosporon asahii, 3 were Cryptococcus laurentii, 1 Rhodotorula mucilaginosa, and 1 was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquatic environments; Yeast microbiota; Candida spp.; Antifungal resistance. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100033 |
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| Waller,Stefanie Bressan; Madrid,Isabel Martins; Ferraz,Vanny; Picoli,Tony; Cleff,Marlete Brum; de Faria,Renata Osório; Meireles,Mário Carlos Araújo; de Mello,João Roberto Braga. |
| Abstract The study aimed to evaluate the anti-Sporothrix sp. activity of the essential oil of Origanum majorana Linn. (marjoram), its chemical analysis, and its cytotoxic activity. A total of 18 fungal isolates of Sporothrix brasiliensis (n: 17) from humans, dogs and cats, and a standard strain of Sporothrix schenckii (n: 1) were tested using the broth microdilution technique (Clinical and Laboratory Standard Institute - CLSI M27-A3) and the results were expressed in minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). The MIC50 and MIC90 of itraconazole against S. brasiliensis were 2 µg/mL and 8 µg/mL, respectively, and the MFC50 and MFC90 were 2 µg/mL and >16 µg/mL, respectively, with three S. brasiliensis isolates... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporotrichosis; Sporothrix schenckii complex; Antifungal resistance; Marjoram; Lamiaceae. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400896 |
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| Waller,Stefanie Bressan; Nakasu,Ceres; Silva,Anna Luiza; Faria,Renata Osório de; Fernandes,João Paulo dos Santos; Cleff,Marlete Brum. |
| From drug repurposing studies, this work aimed to evaluate the activity of different pyrazinoic acid (POA) derivatives against Sporothrix brasiliensis. The POA esters were prepared and characterized as previously reported by classical esterification reactions, with good to excellent yields. Sporothrix brasiliensis isolates from cats (n=6) and standard strains of S. brasiliensis and S. schenckii were used to assess the antifungal activity of the POA derivatives through broth microdilution assay (CLSI M38-A2). Among the tested compounds, molecules 3 and 4 showed fungistatic and fungicidal activities against all Sporothrix spp. strains, and the obtained MIC and MFC values ranged from 2.12 to 4.24 mg/mL and from 1.29 to 5.15 mg/mL, respectively. Compound 2 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporotrichosis; Sporothrix schenckii complex; Itraconazole; Antifungal resistance; Prodrugs; Pyrazinoic acid derivatives. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400630 |
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