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| Griffin, K.; Matthai, E.; Hommel, M.; Weitz, J. C.; Baxby, D.; Hart, C. A.. |
| A panel of monoclonal antibodies has been produced against the protozoan parasite Cryptosporidium parvum which is a major cause of diarrhoeal disease in man and other animals. C. parvum oocysts from both a human and a bovine case of cryptosporidiosis were used as immunogens. A total of 11 different monoclonal antibodies were obtained which could bind to formalin-fixed oocysts. One was IgA but the remainder were all of IgM isotype. The reactivity of these monoclonal antibodies against a series of C. parvum oocysts obtained from 25 patients in Chile was examined using indirect immuno-fluorescence. Although a mixture of all of the 11 antibodies would have detected oocysts in each of the samples, no one monoclonal antibody recognised all oocysts. Each antibody... |
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Palavras-chave: Cryptosporidium parvum; Monoclonal antibody; Antigenic diversity. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/169 |
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| Kreutz,L.C.; Donis,R.; Gil,L.H.V.; Lima,M.; Hoffman,A.N.; Garcez,D.C.; Flores,E.F.; Weiblen,R.. |
| Three Brazilian isolates of bovine viral diarrhea virus (BVDV), antigenically distinct from the standard North American isolates, were selected to immunize BALB/c mice in order to obtain hybridoma cells secreting anti-BVDV monoclonal antibodies (mAbs). Two hybridoma clones secreting mAbs, reacting specifically with BVDV-infected cells (mAbs 3.1C4 and 6.F11), were selected after five fusions and screening of 1001 hypoxanthine-aminopterin-thymidine-resistant clones. These mAbs reacted in an indirect fluorescent antibody (IFA) assay with all 39 South and North American BVDV field isolates and reference strains available in our laboratory, yet failed to recognize other pestiviruses, namely the hog cholera virus. The mAbs reacted at dilutions up to 1:25,600... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine viral diarrhea virus; Monoclonal antibodies; Antigenic diversity; Diagnostic reagents. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001200010 |
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