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| Registros recuperados: 269 | |
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| Cavaliere,Victoria; Glickman,Ariel; Solari,Liliana; Gammella,Daniel; Hajos,Silvia; Álvarez,Élida. |
| La apoptosis es un proceso fisiológico crítico para el crecimiento celular, regulado por factores pro y anti-apoptóticos. Defectos en su control llevan a una ventaja selectiva en la sobrevida celular favoreciendo el desarrollo de patologías neoplásicas. NFkB puede promover la proliferación celular por lo que el uso de inhibidores selectivos lleva a un aumento de la apoptosis. El objetivo del presente trabajo fue caracterizar la línea celular PL104, obtenida de células de punción de médula ósea de una paciente con diagnóstico de leucemia mieloide aguda (LMA) atípica y evaluar la susceptibilidad de la misma frente a los quimioterápicos Doxorubicina (DOX), Vincristina (VCR), Gemcitabina e inhibidores de NFkB, CAPE y MG-132. El fenotipo evaluado por citometría... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leucemia mieloide aguda; Apoptosis; Factor de trascripción nuclear kB; Doxorubicina; Vincristina; Gemcitabina; Ácido cafein fenil etil éster; MG-132. |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572006000100004 |
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| Smaili,Soraya; Hirata,Hanako; Ureshino,Rodrigo; Monteforte,Priscila T.; Morales,Ana P.; Muler,Mari L.; Terashima,Juliana; Oseki,Karen; Rosenstock,Tatiana R.; Lopes,Guiomar S.; Bincoletto,Claudia. |
| Transient increase in cytosolic (Cac2+) and mitochondrial Ca2+ (Ca m2+) are essential elements in the control of many physiological processes. However, sustained increases in Ca c2+ and Ca m2+ may contribute to oxidative stress and cell death. Several events are related to the increase in Ca m2+, including regulation and activation of a number of Ca2+ dependent enzymes, such as phospholipases, proteases and nucleases. Mitochondria and endoplasmic reticulum (ER) play pivotal roles in the maintenance of intracellular Ca2+ homeostasis and regulation of cell death. Several lines of evidence have shown that, in the presence of some apoptotic stimuli, the activation of mitochondrial processes maylead to the release of cytochrome c followed by the activation of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calcium; Apoptosis; Bax; Mitochondria; Endoplasmic reticulum; Neurodegeneration and aging. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300011 |
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| SOARES,JULIANA M.; FARIA,BRUNA M. DE; ASCARI,LUCAS M.; ALVES-LEON,SONIZA V.; SOUZA,JORGE M. DE; SOARES,ANTONIO G.; CORDEIRO,YRAIMA; ROMÃO,LUCIANA F.. |
| Abstract: Diosmin is a flavone glycoside clinically used as the main component of Daflon for the treatment of venous diseases. Several studies demonstrated that this natural compound can induce apoptosis in different tumors. However, isolated diosmin has not been studied regarding its effects on glioblastoma so far. Since glioblastoma is a highly lethal and fast-growing brain tumor, new therapeutic strategies are urgently needed. Herein, we evaluated the role of this flavonoid against glioblastoma cells using in vitro assays. Diosmin significantly reduced the viability of GBM95, GBM02, and U87MG glioblastoma cells, but not of healthy human astrocytes, as verified by MTT assay. Vimentin immunostaining showed that diosmin induced morphological changes in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Diosmin; Flavonoid; Glioblastoma. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700701 |
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| GARCIA,CHARLENE S.C.; MENTI,CAROLINE; LAMBERT,ANA PAULA F.; BARCELLOS,THIAGO; MOURA,SIDNEI; CALLONI,CAROLINE; BRANCO,CÁTIA S.; SALVADOR,MIRIAN; ROESCH-ELY,MARIANA; HENRIQUES,JOÃO A.P.. |
| ABSTRACT Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant; Apoptosis; Cancer; Chemical compounds; Salvia officinalis (L. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100281 |
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| Oliveira,Gisele P.; Dias,Cristina M.; Pelosi,Paolo; Rocco,Patricia R.M.. |
| Glutamine (Gln) is an important energy source and has been used as a supplementary energy substrate. Furthermore, Gln is an essential component for numerous metabolic functions, including acid-base homeostasis, gluconeogenesis, nitrogen transport and synthesis of proteins and nucleic acids. Therefore, glutamine plays a significant role in cell homeostasis and organ metabolism. This article aims to review the mechanisms of glutamine action during severe illnesses. In critically ill patients, the increase in mortality was associated with a decreased plasma Gln concentration. During catabolic stress, Gln consumption rate exceeds the supply, and both plasma and skeletal muscle pools of free Gln are severely reduced. The dose and route of Gln administration... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heat shock protein; Apoptosis; Cytokines; Glutamine. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000200018 |
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| Kanashiro,Alexandre; Andrade,Daiani C.O.; Kabeya,Luciana M.; Turato,Walter M.; Faccioli,Lucia H.; Uyemura,Sérgio A.; Lucisano-Valim,Yara M.. |
| Flavonoids have been reported to exhibit several pharmacological properties, mainly in cardiovascular and inflammatory diseases. In the present study, we observed that rutin, a known glycosylated flavonoid isolated from Dimorphandra mollis, had a lowering effect on plasma triglyceride levels of diet-induced hypercholesterolemic Golden Syrian hamsters, but did not change total cholesterol and high-density lipoprotein cholesterol levels. Moreover, high-fat or rutin supplemented diets showed no immunotoxic effects, since no significant changes were observed on total white blood cells, granulocytes and mononuclear cells, as well as on the neutrophil apoptosis degree, when compared to untreated animals. Therefore, rutin seems to be a selective and non-toxic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rutin; Golden Syrian hamster; Hypercholesterolemia; Atherosclerosis; Apoptosis; Dimorphandra mollis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000100009 |
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| AMIN,BAHAREH; ABNOUS,KHALIL; MOTAMEDSHARIATY,VAHIDEH; HOSSEINZADEH,HOSSEIN. |
| In our previous study, the ethanolic and aqueous extracts of Crocus sativus elicited antinociceptive effects in the chronic constriction injury (CCI) model of neuropathic pain. In this study, we explored anti-inflammatory, anti-oxidant and anti-apoptotic effects of such extracts in CCI animals. A total of 72 animals were divided as vehicle-treated CCI rats, sham group, CCI animals treated with the effective dose of aqueous and ethanolic extracts (200 mg/kg, i.p.). The lumbar spinal cord levels of proinflammatory cytokines including tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β) and interleukin 6 (IL-6), were evaluated at days 3 and 7 after CCI (n=3, for each group). The apoptotic protein changes were evaluated at days 3 and 7 by western blotting.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crocus sativus; Chronic constriction injury; Inflammatory cytokines; Oxidative stress; Apoptosis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401821 |
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| SANTOS,NEYLIANE F.G. DOS; SILVA,RAFAEL F.; PINTO,MARCELA M.P.L.; SILVA,EDVANE B. DA; TASAT,DEBORAH R.; AMARAL,ADEMIR. |
| ABSTRACT Several molecules and events involved in cell response to radiation-induced damage have been investigated towards a personalized radiotherapy. Considering the importance of active caspase-3 in the proteolytic cascade that ensures radiation-induced apoptosis execution, this research was designed to evaluate the expression levels of this protein as a bioindicator of individual radiosensitivity. Peripheral blood samples of 10 healthy individuals were gamma-irradiated (cobalt-60 source) with 1, 2 and 4 Gy (control: non-irradiated samples), and active caspase-3 expression levels were measured in lymphocytes, by flow cytometry, ex vivo and after different times of in vitro incubation (24, 48 and 72 hours). Short-term incubation of 24 h was the most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Active caspase-3; Apoptosis; Flow cytometry; Ionizing radiation; Lymphocytes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200649 |
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| Ferreira-Machado,Samara C.; Salata,Camila; Rocha,Nazareth N.; Corrêa,Alexandre F.S.; Côrte-Real,Suzana; Peregrino,Antônio A.F.; Campos,Vera M.A. de; Andrade,Cherley B.V.; Bernardo-Filho,Mario; Cabral-Neto,Januário B.; Dealmeida,Carlos E.. |
| The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Caspase-3; Fibrosis; Heart; Ionizing radiation; TGF-β1. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100215 |
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| Moro,L.; Vasconcelos,A.C.; Santos,F.G.A.; Alves,C.M.; Nunes,J.E.S.; Sampaio,I.B.M.. |
| Determinou-se o número mínimo de campos por lâmina para se ter uma amostragem representativa para o estudo de apoptose em linfonodos de cães com cinomose. Um linfonodo traqueobrônquico de cão foi colhido e processado segundo técnica para inclusão em parafina. Secções de 5µm foram coradas pelo tricrômico de Shorr. Utilizou-se um analisador de imagens para registrar o número de células em apoptose e os índices apoptóticos de 300 campos, na mesma lâmina. Obtiveram-se, então, valores médios para 10 amostras de 5, 10, 15, 20, 25, 30, 35, 40, 45 e 50 campos ao acaso. Realizou-se o estudo dos desvios-padrão das médias (DP) em relação ao tamanho da amostra que os originou. Quando as oscilações de DP entre amostras consecutivas foram menores que 5%, o ganho em... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Dog; Apoptosis; Morphometry; Lymph node; Sampling. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300020 |
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| Registros recuperados: 269 | |
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