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Validation of spectrophotometric microplate methods for polyphenol oxidase and peroxidase activities analysis in fruits and vegetables Ciênc. Tecnol. Aliment.
SIGUEMOTO,Érica Sayuri; GUT,Jorge Andrey Wilhelms.
Abstract Enzymes polyphenol oxidase (PPO) and peroxidase (POD) play important roles in the processing of fruits and vegetables, since they can produce undesirable changes in color, texture and flavor. Classical methods of activity assessment are based on cuvette spectrophotometric readings. This work aims to propose, to validate and to test microplate spectrophotometric methods. Samples of apple juice and lyophilized enzymes from mushroom and horseradish were analyzed by the cuvette and microplate methods and it was possible to validate the microplate assays with satisfactory results regarding linearity, repeatability, accuracy along with quantitation and detection limits. The proposed microplate methods proved to be reliable and reproducible as the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microplate; Polyphenol oxidase; Peroxidase; Enzymatic assay; Apple juice.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000500148
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Demand Estimation for US Apple Juice Imports: A Restricted Source Differentiated AIDS Model AgEcon
Mekonnen, Dawit Kelemework; Fonsah, Esendugue Greg.
Although this paper focuses on apple juice, a restricted version of source differentiated Almost Ideal Demand System (RSDAIDS) was used to examine U.S. import demand for fresh apple, apple juice and other processed apple. Apple imports were differentiated by type and source of origin and the RSDAIDS model was estimated after imposing the general demand restrictions of adding-up, homogeneity and slutsky symmetry. Seasonality and trend variables were also included on the model. The estimation results showed that U.S. demand for apple juice from China was price inelastic with relatively high expenditure elasticity. We believe the result partially explains why China managed to have a 60 percent import market share in the sub-market despite U.S. imposition of...
Tipo: Conference Paper or Presentation Palavras-chave: Import demand estimation; Apple juice; RSDAIDS; Demand and Price Analysis; International Relations/Trade.
Ano: 2011 URL: http://purl.umn.edu/98789
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