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| Tang,ZhenXing; Qian,JunQing. |
| The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitosan; Gel; Preparation; Application. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200015 |
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| Nagata,T.. |
| A new concept termed "radioautographology" is advocated. This term was synthesized from "radioautography" and "ology", expressing a new science derived from radioautography. The concept of radioautographology (RAGology) is that of a science whose objective is to localize radioactive substances in the biological structure of objects and to analyze and study the significance of these substances in the biological structure. On the other hand, the old term radioautography (RAG) is the technique used to demonstrate the pattern of localization of various radiolabeled compounds in specimens. The specimens used in biology and medicine are cells and tissues. They are fixed, sectioned and placed in contact with the radioautographic emulsions, which are exposed and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Light microscopy; Electron microscopy; Radioautography; Technique; Application. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800001 |
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| ALVES, E. R. de A.; SCHUH, G. E.. |
| Agricultural development efforts quite frequently involve programs designed to extend knowledge to farm people through various kinds of extension programs. Yet, the basic methodology for evaluating such programs is not very well developed. The typical approach is to evaluate the impact of the extension service on various measures of physical productivity, or on other characteristics of technical change. This may involve the calculation of indices of partia1 and total productivity, andlor the collection of data on rates of adoption of new modern inputs or the use of new farm practices. One objective of the present paper is to suggest the application of a methodology which appears to have promise as a means of evaluating the impact of extension programs on a... |
| Tipo: Parte de livro |
Palavras-chave: Aplicacao; Application; Avaliacao; Economic; Evaluation; Metodologia; Minas Gerais; Economia; Brazil; Methodology. |
| Ano: 1984 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/958578 |
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| Nabradi, Andras; Nagymihaly, Kornelia Ficzere. |
| On the basis of our examinations we have come to the conclusion that longitudinal examinations are the simplest and most up-to-date methods as well as the most cost-efficient ones to follow the decision-making, preparatory data collecting, processing and analysing opportunities serving the sustainability of agriculture better and better together with tracing down the feedback about the decisions to see if the given measure has really resulted in reaching the objectives. The method was proved to be able to supply both micro-and macro-level information in the desired breakdown, groupings and systems of connections. The development successive (panel) examination that we applied can serve as the basis of making decisions affecting huge areas by means of total... |
| Tipo: Conference Paper or Presentation |
Palavras-chave: Longitudinal examination; Method; Application; Research Methods/ Statistical Methods. |
| Ano: 2008 |
URL: http://purl.umn.edu/44188 |
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