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| Silberstein,C.; Kierbel,A.; Amodeo,G.; Zotta,E.; Bigi,F.; Berkowski,D.; Ibarra,C.. |
| Water channels or aquaporins (AQPs) have been identified in a large variety of tissues. Nevertheless, their role in the human gastrointestinal tract, where their action is essential for the reabsorption and secretion of water and electrolytes, is still unclear. The purpose of the present study was to investigate the structure and function of water channels expressed in the human colon. A cDNA fragment of about 420 bp with a 98% identity to human AQP3 was amplified from human stomach, small intestine and colon by reverse transcription polymerase chain reaction (RT-PCR) and a transcript of 2.2 kb was expressed more abundantly in colon than in jejunum, ileum and stomach as indicated by Northern blots. Expression of mRNA from the colon of adults and children... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Absorptive epithelium; Human colon; Water channels; Aquaporins; AQP3. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000018 |
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| Ozu,M.; Toriano,R.; Capurro,C.; Parisi,M.. |
| T84 is an established cell line expressing an enterocyte phenotype whose permeability properties have been widely explored. Osmotic permeability (P OSM), hydraulic permeability (P HYDR) and transport-associated net water fluxes (J W-transp), as well as short-circuit current (I SC), transepithelial resistance (R T), and potential difference (deltaV T) were measured in T84 monolayers with the following results: P OSM 1.3 ± 0.1 cm.s-1 x 10-3; P HYDR 0.27 ± 0.02 cm.s-1; R T 2426 ± 109 omega.cm², and deltaV T 1.31 ± 0.38 mV. The effect of 50 µM 5,6-dichloro-1-ethyl-1,3-dihydro-2H-benzimidazol-2-one (DCEBIO), a "net Cl<FONT FACE=Symbol>-</FONT> secretory agent", on T84 cells was also studied. We confirm the reported important increase in I SC induced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Water absorption and secretion; Aquaporins; Osmotic and hydrostatic gradients; T84 cells. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100020 |
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