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La réplication de l'ADN chez l'euryarchaea Pyrococcus Abyssi : mise en place et dynamique du complexe ArchiMer
Rouillon, Christophe.
DNA replication occurs through a large protein complex assembly called replisome. An understanding of its structure and function requires its in vitro reassembly from individual subunits. In all living organisms, the elongation of a primed DNA template by DNA polymerase, with high processivity, requires the accessory protein DNA sliding clamp (PCNA) that is loaded onto DNA by the clamp loader (RF-C). Generally, the archaeal proteins involved in DNA replication are more similar to those of the higher organisms (Eukarya) rather than to those from Bacteria. This study have focussed on the PCNA loading mechanism and on the DNA synthesis in the hyperthermophilic Euryarchaea Pyrococcus abyssi. To investigate the importance of some protein-protein and...
Tipo: Text Palavras-chave: Pyrococcus; Archaea; DNA synthesis; Interaction; RP A; RF C; PCNA; DNA polymerase; DNA replication; Pyrococcus; Archée; Archaea; Synthese d'ADN; Interaction; RP A; RF C; PCNA; ADN polymerase; Replication de l'ADN.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-2461.pdf
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Exploration des communautés virales thermophiles dans les écosystèmes chauds des Terres australes et antarctiques françaises ArchiMer
Parikka, Kaarle Joonas.
Viruses thrive in all types of ecosystems where life is found. They represent the most abundant biological entity of our biosphere. Though several studies have been conducted on viral abundance and dynamics in mesophilic aquatic ecosystems, these aspects remain largely unexplored in extremophilic environments, such as hot springs. In this study, prokaryotic and associated viral communities of the French Southern and Antarctic Lands hot springs were explored. First, prokaryotic cells and Virus-like particles (VLP) were enumerated in several terrestrial and inshore hot springs. The results reveal an abundance of 105 - 106 particles/ml in both types of hot springs studied. The virus-to-prokaryote ratios (VPR) were generally low, confirming thus actual...
Tipo: Text Palavras-chave: Bactérie; Archée; Induction virale; Lysogénie; Source chaude; Abondance; Bacteria; Archaea; Phage; Thermophile; Viral induction; Lysogeny; Hot spring; Abundance.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00609/72139/70891.pdf
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Interactions entre composants de la maintenance génomique chez les Archaea hyperthermophiles: Étude des associations entre PCNA et le complexe Mre11-Rad50 et entre les hélicases MCM et XPD ArchiMer
Hogrel, Gaëlle.
Living at temperatures above 80°C, hyperthermophilic Archaea demonstrated amazing capacity to recover from DNA damages, suggesting they arguably have efficient genome guardians. These guardians, proteins which are relatively similar between Archaea and eukaryotes, act and interact like a ballet orchestrated by the cell. Several proteins involved in essential repair pathway in eukaryotes are missing in Archaea. To gain insights into archaeal genome maintenance processes, a previous work proposed a protein-protein interaction network based on Pyrococcus abyssi proteins. Through this network, new interactions involving proteins from DNA replication and proteins from DNA repair were highlighted. To describe interactions for a better understanding of their...
Tipo: Text Palavras-chave: Archée; Réplication et Recombinaison de l’ADN; Interaction protéine-protéine.; Archaea; DNA replication; DNA repair & recombination; Protein-protein interaction..
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00300/41158/40351.pdf
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