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Ferreira,Enderson Petrônio de Brito; Dusi,André Nepomuceno; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa. |
The objective of this work was to determine the shifts on the PCR-DGGE profiles of bacterial communities associated to the rhizosphere of potato cultivars, in order to generate baseline information for further studies of environmental risk assessment of genetically modified potato plants. A greenhouse experiment was carried out with five potato cultivars (Achat, Bintje, Agata, Monalisa and Asterix), cultivated in pots containing soil from an integrated system for agroecological production. The experiment was conducted in a split plot randomized block design with five cultivars, three sampling periods and five replicates. Rhizosphere samples were collected in three sampling dates during plant development. DNA of rhizosphere microorganisms was extracted,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; Associative bacteria; Molecular marker; PCA. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500008 |
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Braga,Lívia Fabiana; Oliveira,Fênix Araújo de; Couto,Eva Aparecida Prado do; Santos,Karina Freire d'Eça Nogueira; Ferreira,Enderson Petrônio de Brito; Martin-Didonet,Claudia Cristina Garcia. |
ABSTRACT This work aimed to characterize 20 isolates obtained from upland rice plants, based on phenotypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16S rRNA sequencing) and plant growth promotion. Results showed a great morphological, metabolic and genetic variability among bacterial isolates. All isolates showed positive activity for catalase and protease enzymes and, 90% of the isolates showed positive activity for amylase, catalase and, nitrogenase. All isolates were able to metabolize sucrose and malic acid in contrast with mannitol, which was metabolized only by one isolate. For the other carbon sources, we observed a great variability in its use by the isolates. Most... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; PGPR; Associative bacteria; Bacterial diversity. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100020 |
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