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A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors Electron. J. Biotechnol.
Yulita,Kusumadewi Sri; Kouno,Takafumi; Ezaki,Bunichi.
Here we introduce a new approach for the screening of DNA binding proteins, using a phage library based on a phage display technique. In principal, a complementary DNA (cDNA) library based on the recombinant bacteriophage T7 expressing target proteins on its capsid (phage display) is constructed. These phage particles are hybridized with a biotinylated target DNA fragment which is immobilized on the surface of streptavidin paramagnetic particle (SA-PMP). The phage particles are released from the target DNA fragment by a nuclease treatment and the recovered phages are used to the next round of hybridization. These processes are repeated three times to amplify the target phages in the population. This simple method is faster, and more systemic than other...
Tipo: Journal article Palavras-chave: AtGST11 gene; Biopanning; DNA binding proteins; T7 phage differential display; Transcription factors.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100014
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