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Physicochemical and Biological Investigation of Different Structures of Carbon Coatings Deposited onto Polyurethane BABT
Kaczorowski,Witold; Batory,Damian; Jakubowski,Witold; Szymanski,Witold; Komorowski,Piotr; Walkowiak,Bogdan; Sanak,Marek; Niedzielski,Piotr.
The aim of this study was to examine the thrombogenic properties of polyurethane that was surface modified with carbon coatings. Physicochemical properties of manufactured coatings were investigated using transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS), Raman spectroscopy and contact angle measurement methods. Samples were examined by the Impact-R method evaluating the level of platelets activation and adhesion of particular blood cell elements. The analysis of antimicrobial resistance against E. coli colonization and viability of endothelial cells showed that polyurethane modified with use of carbon layers constituted an interesting solution for biomedical application.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diamond-like carbon; Atomic force microscopy (AFM); X-ray photoelectron spectroscopy XPS; Raman spectroscopy; Biological properties.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100600
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Measuring bacterial cells size with AFM BJM
Osiro,Denise; Bernardes Filho,Rubens; Assis,Odilio Benedito Garrido; Jorge,Lúcio André de Castro; Colnago,Luiz Alberto.
Atomic Force Microscopy (AFM) can be used to obtain high-resolution topographical images of bacteria revealing surface details and cell integrity. During scanning however, the interactions between the AFM probe and the membrane results in distortion of the images. Such distortions or artifacts are the result of geometrical effects related to bacterial cell height, specimen curvature and the AFM probe geometry. The most common artifact in imaging is surface broadening, what can lead to errors in bacterial sizing. Several methods of correction have been proposed to compensate for these artifacts and in this study we describe a simple geometric model for the interaction between the tip (a pyramidal shaped AFM probe) and the bacterium (Escherichia coli JM-109...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Atomic force microscopy (AFM); Escherichia coli; Cell dimensions; Bacteria visualization.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100040
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