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Validation d’un procédé automatisé d’extraction sur phase solide de glandes digestives de moules pour l’identification et la quantification des dinophysistoxines en LC/ESI/SM2 par piégeage d’ions quadripolaire ArchiMer
Mondeguer, Florence; Mestres, Gilberte; Rouland, Caroline; Montagu, Monique; Maillols, Hélène.
This report presents a new extraction method of the dinophysistoxins (DTXs), confirmed by quantification using high-performance liquid chromatography coupled to mass spectrometry with an ion trap and electro spray interface (HPLC/ESI/MS2). The method originality consists on the adaptation of DTXs basic extraction procedure (liquid/ liquid) to a solid phase extraction (SPE) via a robotic station: ASPEC XLi The parameters of the automatization procedure were optimized to obtain the best DTXs recovery rate. These improvements were loaded with digestive gland mussel homogenat realized on a silica cartridge SPE, activated in hexane/chloroform (50:50), washed with hexane/chloroform (50:50) and extracted by an elution gradient (chloroform methanol (65:35) and...
Tipo: Text Palavras-chave: Toxines; Intoxication des coquillages; Dinophysistoxines; Acide okadaïque; Dinophysistoxine-1; Méthode alternative; Extraction sur phase solide; Automatisation; Couplage CLHP/ESI/SM2 par piège d’ions.; Toxins; Shellfish poisoning; Dinophysistoxins; Okadaic acid; Dinophysistoxin-1; Alternative method; Solid phase extraction; Automation; Liquid chromatography coupled ion trap..
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00342/45270/44724.pdf
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Sélection de paramètres physiques pour la régulation et la maîtrise de la distribution de nourriture (Skeletonema costatum) aux coquillages d'intérêt commercial ArchiMer
Haure, Joel; Palvadeau, Hubert; Nourry, Max; Penisson, Christian; Martin, Jean-louis.
A study was realized to automate the distribution of phytoplankton (Skeletonema costatum) in the extensive breedings of shells of commercial interest. Physical parameters were selected to define their domain of application for the realization of such a project The work was realized in spring (in June, 1999) and in autumn (in November, 1999) in different dilution of phytoplankton in a treated subterranean salt water. The results showed that the fluorescence gave a better sensibility to inter seasonal in the explanation of the distributed food, but lost as representation during the night-phases, On the other hand, the absorption ( optical density) presented less daily variability and with good correlations with the nourishing concentration of the environment...
Tipo: Text Palavras-chave: Fluorescence in vivo; Absorption in vivo; Automatisation; Skeletonema costatum; Fluorescence in vovo; Absorption in vivo; Automation; Skeletonema costatum.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00047/15870/13289.pdf
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