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	Provedor de dados OAK (2) Autor Hashimoto, Makoto (2) Hatanaka, Yasumaru (2) Nabeta, Kensuke (1) Okamoto, Shun’ji (1) Palavra-chave Avidin-biotin (2) Diazirine (2) Chemical biology (1) Chymotrypsin (1) Photoaffinity label (1) Photoaffinity labeling (1) Staudinger reaction (1) V8 protease (1) Mais... Ano 2005 (1) 2004 (1) País Japan (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Post-biotinylation of Photocrosslinking by Staudinger-Bertozzi Ligation of Preinstalled Alkylazide Tag OAK Hashimoto, Makoto; Hatanaka, Yasumaru. Post-biotinylation of the alkyl azide derivative of trifluoromethyl phenyldiazirine (TPD) was elucidated to apply a photoaffinity biotinylation technique. A photo-modified polyvinilidene difluoride (PVDF) membrane was used as a photolabeled component and we introduced biotin by Staudinger-Bertozzi ligation. The 15 pmol amount of biotinylated reagent was still effective for the visualization of cross-linked product on the matrix. The results show the potential utility of alkyl azide carrying TPD derivatives in the application of photoaffinity biotinylation, which could be useful for the ligands with tight structural requirements. Palavras-chave: Diazirine; Photoaffinity label; Chemical biology; Staudinger reaction; Avidin-biotin. Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/832 Enzyme cleavable and biotinylated photoaffinity ligand with diazirine OAK Hashimoto, Makoto; Okamoto, Shun’ji; Nabeta, Kensuke; Hatanaka, Yasumaru. The efficient synthesis of an enzyme cleavable biotinylated diazirinyl photoaffinity ligand is described to allow the effective manipulation of the photolabeled biocomponents. The compound contains a glutamic acid gamma-methyl ester, which is a precursor of the substrate for V8 protease, between the diazirinyl photophor and biotin moiety. After alkaline hydrolysis of the ester, the compound can be proteolyzed at the Glu moiety by V8 protease. The photophore was introduced to L-Phe p-nitroanilide and the ligand was applied to photolabel of chymotrypsin to manipulate the application of the concept. Palavras-chave: Photoaffinity labeling; Diazirine; Avidin-biotin; V8 protease; Chymotrypsin. Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/738 Registros recuperados: 2 Primeira ... 1 ... Última

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