|
|
| BULAT,TANJA; KETA,OTILIJA; KORIĆANAC,LELA; ŽAKULA,JELENA; PETROVIĆ,IVAN; RISTIĆ-FIRA,ALEKSANDRA; TODOROVIĆ,DANIJELA. |
| ABSTRACT Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (γH2AX). Immunofluorescent staining visualizes formation of γH2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of γH2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to γ-rays, in the dose range from 1 to 16 Gy. Radiation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ApoTome software; AxioImagerA1 microscope; Immunofluorescence microscopy; Western blot; ΓH2AX. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100127 |
| |
