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	Provedor de dados BABT (1) BJM (1) Autor Alfieri,Alice Fernandes (2) Alfieri,Amauri Alcindo (2) Barreiros,Marco Antônio Bacellar (1) Barry,Aline Fernandes (1) Stipp,Danilo Tancler (1) Takiuchi,Elisabete (1) Mais... Palavra-chave BCoV (2) Calf (1) Cleavage site (1) Co-infection (1) Diarrhea (1) Mutation (1) Rotavirus (1) SN-PCR (1) Sequencing (1) Spike protein (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2007 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Bovine coronavirus detection in a collection of diarrheic stool samples positive for group a bovine rotavirus BABT Barry,Aline Fernandes; Alfieri,Alice Fernandes; Stipp,Danilo Tancler; Alfieri,Amauri Alcindo. Neonatal diarrhea is an important cause of economic losses for cattle farmers. The main viral etiologies of enteric diseases are group A rotaviruses (GARV) and the bovine coronavirus (BCoV). Although both viruses infect calves of the same age, the occurrence of mixed infections is still under studied. The present study describes the co-infection of BCoV and GARV in stool samples. Forty-four diarrheic fecal samples from calves up to 60 days old that had previously tested positive for GARV by SS-PAGE were analyzed using semi-nested PCR for BCoV. A product with 251 bp of the BCoV nucleoprotein gene was amplified in 15.9% (7/44) of the samples, demonstrating that co-infection is not an unusual event. These results reinforce the need for testing for both GARV... Tipo: Info:eu-repo/semantics/article Palavras-chave: Calf; Diarrhea; BCoV; Rotavirus; SN-PCR; Co-infection. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700006 Identification of a mutation in the spike protein cleavage site in Brazilian strains of wild-type bovine coronavirus BJM Takiuchi,Elisabete; Barreiros,Marco Antônio Bacellar; Alfieri,Alice Fernandes; Alfieri,Amauri Alcindo. The spike (S) protein of coronaviruses, a type I membrane glycoprotein, is primarily responsible for entry into susceptible cells by binding with specific receptors on cells and mediating subsequent virus-cell fusion. The bovine coronavirus (BCoV) S protein is cleaved into two subunits, the N-terminal S1 and the C-terminal S2. The proteolytic cleavage site of S protein is highly conserved among BCoV strains and is located between amino acids 763 and 768 (KRRSRR). This study describes a single mutation in the S protein cleavage site of three Brazilian strains of BCoV detected in diarrheic fecal samples from calves naturally infected. The sequenced PCR products revealed that amino acid sequence of the cleavage site of our strains was KRRSSR, indicating a... Tipo: Info:eu-repo/semantics/article Palavras-chave: BCoV; Sequencing; Spike protein; Cleavage site; Mutation. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400021 Registros recuperados: 2 Primeira ... 1 ... Última

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