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	Provedor de dados BJMBR (1) Autor Guo,Q.F. (1) Li,X.Y. (1) Liu,Q.H. (1) Liu,S.H. (1) Xu,H.H. (1) Palavra-chave Adenovirus (1) BFGF (1) BMP2 (1) BMPCs (1) Osteogenic differentiation (1) T2A sequence (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2013 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor BJMBR Xu,H.H.; Liu,S.H.; Guo,Q.F.; Liu,Q.H.; Li,X.Y.. Bone morphogenetic protein 2 (BMP2) and basic fibroblast growth factor (bFGF) have been shown to exhibit a synergistic effect to promote bone repair and healing. In this study, we constructed a novel adenovirus with high coexpression of BMP2 and bFGF and evaluated its effect on osteogenic differentiation of goat bone marrow progenitor cells (BMPCs). Recombinant adenovirus Ad-BMP2-bFGF was constructed by using the T2A sequence. BMPCs were isolated from goats by density gradient centrifugation and adherent cell culture, and were then infected with Ad-BMP2-bFGF or Ad-BMP2. Expression of BMP2 and bFGF was detected by ELISA, and alkaline phosphatase (ALP) activity was detected by an ALP assay kit. In addition, von Kossa staining and immunocytochemical staining... Tipo: Info:eu-repo/semantics/article Palavras-chave: BMP2; BFGF; Adenovirus; T2A sequence; BMPCs; Osteogenic differentiation. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900809 Registros recuperados: 1 Primeira ... 1 ... Última

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