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DNA barcoding reveals the diversity of sharks in Guyana coastal markets Neotropical Ichthyology
Kolmann,Matthew A.; Elbassiouny,Ahmed A.; Liverpool,Elford A.; Lovejoy,Nathan R..
ABSTRACT A fundamental challenge for both sustainable fisheries and biodiversity protection in the Neotropics is the accurate determination of species identity. The biodiversity of the coastal sharks of Guyana is poorly understood, but these species are subject to both artisanal fishing as well as harvesting by industrialized offshore fleets. To determine what species of sharks are frequently caught and consumed along the coastline of Guyana, we used DNA barcoding to identify market specimens. We sequenced the mitochondrial co1 gene for 132 samples collected from six markets, and compared our sequences to those available in the Barcode of Life Database (BOLD) and GenBank. Nearly 30% of the total sample diversity was represented by two species of Hammerhead...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BOLD; Carcharhinidae; Elasmobranch; Guianas; Sphyrnidae.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000400209
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Using DNA barcodes to identify forensically important species of Diptera in Espírito Santo State, Brazil BABT
Oliveira,Pablo Viana; Matos,Natan Silva; Klippel,Angélica Hollunder; Oliveira-Costa,Janyra; Careta,Francisco de Paula; Paneto,Greiciane Gaburro.
ABSTRACT Accurate insect specimen identification is usually a crucial first step in a forensic entomological analysis. It is traditionally done by morphological classification using identification keys. However, due to sensibility limitations in the identification of animal species based only on their morphology, new methods have been developed, including species identification by DNA barcodes. The objective of this study was to identify forensically important species of Diptera in Espirito Santo state using DNA barcodes. For this, adult flies were collected in Espirito Santo, Southeast Region of Brazil. After DNA extraction, COI gene was amplified and sequenced. All sequences were matched to BOLD platform and alternatively to GenBank MegaBLAST. As result,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomology; COI; BOLD; Forensic; Flies.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100403
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