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| Ma,Yanlin; Deng,Yang; Xu,Zhenbo; Liu,Junyan; Dong,Jianjun; Yin,Hua; Yu,Junhong; Chang,Zongming; Wang,Dongfeng. |
| ABSTRACT The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this paper, the combination of propidium monoazide (PMA) pretreatment and conventional PCR had been described. The established PMA-PCR identified beer spoilage Lactobacillus brevis based not on their identity, but on the presence of horA gene which we show to be highly correlated with the ability of beer spoilage LAB to grow in beer. The results suggested that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propidium monoazide; Polymerase chain reaction; Beer spoilage bacteria; Lactobacillus brevis horA. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400740 |
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