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| Iracheta-Cárdenas,María M.; Almeyda-León,Isidro H.; Cevik,Bayarm; Niblett,Charles L.; Lee,Richard F.; Rocha-Peña,Mario A.. |
| Citrus tristeza virus (CTV) is an important issue to the Mexican citrus industry. CTV infected plants have been reported in 20 citrus producing states of the country as symptomless virus infections, even on plants grafted on the sensitive sour orange rootstock. The detection of severe CTV isolates is mandatory to reinforce the operation of the CTV campaign nationwide. In this study, 13 CTV isolates from Nuevo León and one from Tamaulipas states were characterized for activity against the strain discriminatory MCA13 monoclonal antibody, bi-directional (BD) PCR, and hybridization with specific DNA probes, with RT-PCR amplicons, of the p25 coat protein gene. Ten out of 13 CTV isolates from Nuevo León and the Tamaulipas isolate gave a positive reaction to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bi-directional PCR; DIBA; Hybridization; MCA 13 monoclonal antibody. |
| Ano: 2010 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000400005 |
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