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Registros recuperados: 97
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Tolerancia y biodegradación de plaguicidas con hongos filamentosos. Colegio de Postgraduados
Stamatiu Sánchez, Katina.
El uso indiscriminado y las malas prácticas de manejo de plaguicidas en la producción agrícola, han llevado a la contaminación de todos los ambientes debido a su fácil dispersión y representan un riesgo para la salud humana y fauna silvestre. Plaguicidas como el endosulfán (EN), clorpirifós (CRP) y clorotalonil (CTL) son de amplio uso en diferentes cultivos, están catalogados como carcinogénicos y disruptores endócrinos. El uso de microorganismos para degradar plaguicidas ha permitido implementar tecnologías de biorremediación conocidos como “biobeds”, en las que se busca acelerar la biodegradación con biomezclas de suelo, paja de trigo y composta. En esta investigación se evaluó la tolerancia y capacidad de degradación de hongos filamentosos provenientes...
Palavras-chave: Endosulfán; Clorpirifós; Contaminación; Hongos filamentosos; Tolerancia; Biodegradación; Chlorpyrifos; Contamination; Filamentous fungi; Tolerance; Biodegradation; Entomología y Acarología; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/2175
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Biodegradation of deproteinized potato wastewater and glycerol during cultivation of Rhodotorula glutinis yeast Electron. J. Biotechnol.
Kot,Anna Maria; Blazejak,Stanislaw; Kurcz,Agnieszka; Gientka,Iwona.
Background Deproteinized potato wastewater and glycerol are two by-products which are difficult to dispose. The objective of this study was to determine the ability of Rhodotorula glutinis to use glycerol and nitrogen compounds present in deproteinized potato wastewater and to evaluate the ability of simultaneous biodegradation of potato wastewater and glycerol via microbiological methods. Results It has been found that R. glutinis used glycerol and potato wastewater as a source of carbon and nitrogen, respectively. The highest degree of glycerol content (70.6%) reduction was found after cultivation of the investigated strain using a medium with 5% glycerol. In this medium, a significant reduction in the total protein content, estimated at 61%, was...
Tipo: Journal article Palavras-chave: Biodegradation; Chemical oxygen demand; Industrial waste; Rhodotorula.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600007
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Stenotrophomonas maltophilia isolated from gasoline-contaminated soil is capable of degrading methyl tert-butyl ether Electron. J. Biotechnol.
Alfonso-Gordillo,Guadalupe; Cristiani-Urbina,Eliseo; Flores-Ortiz,César Mateo; Peralta,Humberto; Cancino-Díaz,Juan Carlos; Cruz-Maya,Juan Antonio; Jan-Roblero,Janet.
Background: Methyl tert-butyl ether (MTBE) is a pollutant that causes deleterious effects on human and environmental health. Certain microbial cultures have shown the ability to degrade MTBE, suggesting that a novel bacterial species capable of degrading MTBE could be recovered. The goal of this study was to isolate, identify and characterize the members of a bacterial consortium capable of degrading MTBE. Results: The IPN-120526 bacterial consortium was obtained through batch enrichment using MTBE as the sole carbon and energy source. The cultivable fraction of the consortium was identified; of the isolates, only Stenotrophomonas maltophilia IPN-TD and Sphingopyxis sp. IPN-TE were capable of degrading MTBE. To the best of our knowledge, this report is the...
Tipo: Journal article Palavras-chave: Bacterial consortium; Biodegradation; MTBE; Sphingopyxis sp; Stenotrophomonas maltophilia.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500003
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Chloroperoxidase mediated oxidation of chlorinated phenols using electrogenerated hydrogen peroxide Electron. J. Biotechnol.
La Rotta,Camilo E; D'Elia,Eliane; Bon,Elba P.S.
Chloroperoxidase (CPO) from Caldariomyces fumago catalyses the oxidation of several chlorinated phenols (CP) commonly found in industrial waste waters in the presence of hydrogen peroxide. This study compares the direct addition of hydrogen peroxide (DA) with its continuous electrogeneration (EG) during the enzymatic oxidation of CP. Reaction mixtures were studied containing chemically modified CPO, hydrogen peroxide and the phenolic substrates: phenol (P), 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP), in 100 mM sodium-potassium phosphate buffer pH 6.0, at 25ºC. Results were compared in terms of residual phenol concentration (oxidation efficiency), precipitate formation (removal) and...
Tipo: Journal article Palavras-chave: Biodegradation; Bioelectrochemistry; Caldariomyces fumago; Chlorinated phenols; Chloroperoxidase; Hydrogen peroxide electrogeneration.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100003
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Utilization and biodegradation of starch stillage (distillery wastewater) Electron. J. Biotechnol.
Krzywonos,Malgorzata; Cibis,Edmund; Miskiewicz,Tadeusz; Ryznar-Luty,Agnieszka.
Stillage (distillery wastewater) is the main by-product originating in distilleries, and its volume is approximately 10 times that of ethanol produced. It is not surprising that the utilization of the stillage raises serious problems, and that many attempts have been made all over the world to solve them. In Poland most of the ethanol (about 90%) is produced from starch-based feedstocks, i.e. grains and potatoes. Starch feedstocks are widely used for spirit production also in other European countries, as well as outside Europe. The manuscript provides an overview of global fuel ethanol production and information on methods used for starch-based stillage biodegradation and utilization. The methods presented in this paper have been classified into two major...
Tipo: Journal article Palavras-chave: Biodegradation; Distillery; Ethanol; Starch stillage; Utilization.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100006
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Biotransformation of anthracene and fluoranthene by Absidia fusca Linnemann Electron. J. Biotechnol.
Villemain,Danièle; Guiraud,Pascale; Bordjiba,Ouahiba; Steiman,Régine.
A strain of Absidia fusca was isolated from a pesticide-contaminated soil (Annaba, Algeria). The biotransformation capability of this strain towards two polycyclic aromatic hydrocarbons (PAHs): anthracene and fluoranthene was compared to that exhibited by another strain of A. fusca isolated from a non-contaminated milieu and considered as a control. The results obtained were statistically analyzed and showed that the strain isolated from the contaminated soil was more efficient than the control to remove anthracene from the medium, during all the kinetics (90% removed versus 45% after 24 hrs). Concerning fluoranthene, the amount removed by both strains was very high during the first 24 hrs however the control strain was slightly more efficient (94% versus...
Tipo: Journal article Palavras-chave: Absidia fusca; Anthracene; Biodegradation; Fluoranthene; Kinetics.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200004
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Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation Electron. J. Biotechnol.
Hernández García,Marcela; Morgante,Verónica; Ávila Perez,Marcela; Villalobos Biaggini,Patricio; Miralles Noé,Pola; González Vergara,Myriam; Seeger Pfeiffer,Michael.
s-Triazine-degrading bacterial strains were isolated from long-term simazine-treated agricultural soils of central Chile. The number of culturable heterotrophic bacteria of these agricultural soils (7 x 10(6) CFU/g of dry soil) was not affected by simazine application on field. The simazine-degrading bacterial strains P51, P52 and C53 were isolated by enrichment in minimal medium using simazine as the sole nitrogen source. Resting cells of strains P51 and P52 degraded >80% of simazine within 48 hrs, whereas strain C53 was able to remove >60% of the herbicide. The atzA and atzD genes of the s-triazine upper and lower catabolic pathways were detected in strains P51 and C53, while only atzD gene was observed in strain P52. To compare the bacterial 16S...
Tipo: Journal article Palavras-chave: Arthrobacter; Biodegradation; Bioremediation; Simazine; Stenotrophomonas; S-triazine.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500005
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Biodegradation of a blended starch/natural rubber foam biopolymer and rubber gloves by Streptomyces coelicolor CH13 Electron. J. Biotechnol.
Watcharakul,Sirimaporn; Umsakul,Kamontam; Hodgson,Brian; Chumeka,Wannapa; Tanrattanakul,Varaporn.
Background: The growing problem of environmental pollution caused by synthetic plastics has led to the search for alternative materials such as biodegradable plastics. Of the biopolymers presently under development, starch/natural rubber is one promising alternative. Several species of bacteria and fungi are capable of degrading natural rubber and many can degrade starch. Results: Streptomyces coelicolor CH13 was isolated from soil according to its ability to produce translucent halos on a mineral salts medium, MSM, supplemented with natural rubber and to degrade starch. Scanning electron microscope studies showed that it colonized the surfaces of strips of a new starch/natural rubber biopolymer and rubber gloves and caused degradation by forming holes,...
Tipo: Journal article Palavras-chave: Biodegradation; Biopolymer; Natural rubber; Starch; Streptomyces coelicolor.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100008
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Degradation of citronellol, citronellal and citronellyl acetate by Pseudomonas mendocina IBPse 105 Electron. J. Biotechnol.
Tozoni,Daniela; Zacaria,Jucimar; Vanderlinde,Regina; Delamare,Ana Paula Longaray; Echeverrigaray,Sergio.
The purpose of this work was to stud the biodegradation of citronellol, citronellal and citronellyl acetate by a soil Pseudomonas mendocina strain (IBPse 105) isolated from a Cymbopogon windelandi field. This strain efficiently used citronellol, citronellal, citronellyl acetate and myrcene as sole source of carbon, but was not able to grow on other 15 monoterpenoids evaluated. Gas chromatography-mass spectrometry (GC-MS) analysis of metabolites accumulation during P. medocina IBPse 105 growth on citronellol showed that this strain uses the citronellol catabolic pathway described for other species of the genus. IBPse 105 degradation of citronellyl acetate initiates by its hydrolysis to citronellol. The mini-Tn5 insertion in mutant IBPse 105-303, impaired in...
Tipo: Journal article Palavras-chave: Biodegradation; Citronellol catabolism; Monoterpenes degradation; P. mendocina.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200002
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Biosorption of heavy metals by Talaromyces helicus: a trained fungus for copper and biphenyl detoxification Electron. J. Biotechnol.
Romero,M. Cristina; Reinoso,Enso H.; Urrutia,M. Inés; Moreno Kiernan,Alejandro.
At present, it is common to observe environments with organic and inorganic pollution, defined as co-contamination. Most industrial and urban effluents releases both pollutant types, leading to a complex environmental problem, as the biota must be tolerant to both xenobiotics. T. helicus, an efficient strain to degrade biphenyl, was trained with high copper levels, and became co tolerant to cobalt, lead and cadmium when was cultured in their presence. The copper adaptation was the result of physiological mechanisms, and the activated biochemical processes conferred resistance to Cu2+ as well as to other heavy metals. Furthermore, the Cu2+ adaptation of the mycelium was also transferred to the spores, that removed twice as much copper from solution than...
Tipo: Journal article Palavras-chave: Biodegradation; Biphenyl; Cocontaminated sites; Cotolerance; Metal sorption; Talaromyces helicus.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300009
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Biodegradation kinetics of o-cresol by Pseudomonas putida DSM 548 (pJP4) and o-cresol removal in a batch-recirculation bioreactor system Electron. J. Biotechnol.
Kaymaz,Yasin; Babaoglu,Anil; Pazarlioglu,Nurdan Kasikara.
The biodegradation kinetics of o-cresol was examined by acclimatized P. putida DSM 548 (pJP4) in batch experiments at varying initial o-cresol concentrations (from 50 to 500 mg/L). The kinetic parameters of o-cresol aerobic biodegradation were estimated by using the Haldane substrate inhibition equation. The biodegradation kinetics of o-cresol was investigated. In batch culture reactors, the Maximum specific growth rate (μmax), Monod constant (Ks) and the inhibition constant (Ki) were established as 0.519 h-1, 223.84 mg/L and 130.883 mg/L, respectively. o-cresol biodegradation in a batch-recirculation bioreactor system by immobilized P. putida was also studied. The recycled packed bed reactor system, which was composed of Ca-alginate beads and...
Tipo: Journal article Palavras-chave: Biodegradation; Cell immobilization; O-cresol; Pseudomonas putida; Substrate inhibition kinetics.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100003
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Banco de Extratos de Enzimas Fibrolíticas Isoladas de Conteúdo Ruminal (BEEFRUM). Infoteca-e
SALLES, H. O.; EGITO, A. S. do; CARNEIRO, J. da C.; XIMENES, L. V.; RIBEIRO, M. T.; RIBEIRO, R. P.; BARBOSA, I. C.; SOUSA, A. M. P..
A criação do banco de extratos composto por enzimas fibrolíticas isoladas de conteúdo ruminal de bovinos, caprinos e ovinos, foi iniciativa da Embrapa Caprinos e Ovinos e da Embrapa Gado de Leite, através de projetos de pesquisa em parceria, e tem como objetivo salvaguardar extratos enzimáticos de nossa biodiversidade para utilização biotecnológica, visando caracterizar, avaliar e identificar possíveis aplicabilidades científicas e/ou industriais das enzimas contidas nesses extratos.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Bioetanol; Enzimas fibrolíticas; Sugar byproducts.; Cellulolysis; Etanol; Cana de açúcar; Bagaço; Celulase; Enzima celulolítica; Cellulose; Biodegradation; Sugarcane; Ethanol..
Ano: 2017 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1087003
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Degradação de atrazina por fungos filamentosos. Infoteca-e
MELO, I. S. de; SILVA, C. M. M. de S.; FAY, E. F.; MONTEIRO, R. R.; ROSAMIGLIA, A. C..
Atrazina é um dos herbicidas mais utilizados no mundo, apresentando relativa persistência em zonas saturadas e insaturadas. A principal via de dissipação de atrazina inclui a biodegradação. Solos coletados no município de Guaíra, SP, provenientes de áreas de mata, e solos sob cultivo intensivo, sob irrigação, com histórico de aplicação de atrazina, foram suplementados e incubados com o herbicida nas seguintes concentrações:70 microgramas g-1, 350 microgramas g-1 700 microgramas g-1. Após diluição em série, procedeu-se ao plaqueamento em meio de cultura (meio de Martin), também suplementado com o herbicida (700~g g ' l). Os fungos selecionados nesta fase foram cultivados em meio líquido de batata- dextrose (50%) suplementado com 700 microgramas mL-1 de...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Herbicida; Microrganismo; Atrazina; Biodegradação; Penicillium; Atrazine; Herbicides; Biodegradation.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1026398
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When riverine dissolved organic matter (DOM) meets labile DOM in coastal waters: changes in bacterial community activity and composition ArchiMer
Blanchet, Marine; Pringault, Olivier; Panagiotopoulos, Christos; Lefevre, Dominique; Charriere, Bruno; Ghiglione, Jean-francois; Fernandez, Camila; Aparicio, Fran L.; Marrase, Celia; Catala, Philippe; Oriol, Louise; Caparros, Jocelyne; Joux, Fabien.
Heterotrophic bacterial communities in marine environments are exposed to a heterogeneous mixture of dissolved organic compounds with different bioreactivity that may control both their activity and composition. The coastal environment is an example of a mixing area where recalcitrant allochthonous organic matter from rivers can encounter labile organic matter from marine phytoplanktonic blooms. The objective of this study was to explore the effects of mixed qualities of dissolved organic matter (DOM) on bacterial community activity (BCA) and bacterial community composition (BCC) and to test for a priming effect when DOM sources are added in combination. Coastal marine bacterial communities were incubated separately with a mixture of amino acids and with...
Tipo: Text Palavras-chave: Dissolved organic matter; Biodegradation; Coastal waters; Bacterial community composition; Priming effect.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00625/73737/74701.pdf
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Capacity of the potentially toxic diatoms Pseudo-nitzschia mannii and Pseudo-nitzschia hasleana to tolerate polycyclic aromatic hydrocarbons ArchiMer
Ben Garali, Sondes Melliti; Sahraoui, Ines; Ben Othman, Hiba; Kouki, Abdessalem; De La Iglesia, Pablo; Diogene, Jorge; Lafabrie, Celine; Andree, Karl B.; Fernandez-tejedor, Margarita; Mejri, Kaouther; Meddeb, Marouan; Pringault, Olivier; Hlaili, Asma Sakka.
This study investigates the effects of polycyclic aromatic hydrocarbons (PAHs) on two potentially toxic Pseudonitzschia hasleana and P. mannii, isolated from a PAH contaminated marine environment. Both species, maintained in non-axenic cultures, have been exposed during 144 h to increasing concentrations of a 15 PAHs mixture. Analysis of the domoic acid, showed very low concentrations. Dose?response curves for growth and photosynthesis inhibition were determined. Both species have maintained their growth until the end of incubation even at the highest concentration tested (120 ?g l-1), Nevertheless, P mannii showed faster growth and seemed to be more tolerant than P. hasleana. To reduce PAH toxicity, both species have enhanced their biovolume, with a...
Tipo: Text Palavras-chave: Diatoms; Pseudo-nitzschia; PAHs mixtures; Biodegradation; Eco-toxicology.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00690/80215/83300.pdf
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Natural Degradation and Biodegradation of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) in Liquid and Solid Marine Environments ArchiMer
Deroine, Morgan; Cesar, Guy; Le Duigou, Antoine; Davies, Peter; Bruzaud, Stephane.
In this study, natural degradation and biodegradation of poly(3-hydroxybuyrate-co-3-hydroxyvalerate) (PHBV) films were followed in different marine environments. First of all, ageing of PHBV films was investigated in natural seawater for 180 days and degradation was followed by means of weight loss measurements, scanning electron microscopy (SEM), differential scanning calorimetry and steric exclusion chromatography. In a second part, biodegradation tests were performed on PHBV powder, by following carbon dioxide (CO2) release(,) to highlight the PHBV bioassimilation of marine microorganisms. Three different marine environments were considered for biodegradation tests: a solid inoculum with foreshore sand, a solid-liquid inoculum with sand and seawater and...
Tipo: Text Palavras-chave: PHBV; Natural ageing; Degradation; Biodegradation; Seawater.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00296/40735/42020.pdf
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Taxonomy and distribution of hydrocarbonoclastic bacteria from the Ixtoc-1 area. ArchiMer
Lizarraga, P; Munoz, R; Porras, A; Izquierdo, V; Wongchang, I.
A bacteriological monitoring program has been established in order to evaluate the impact of oil activities. The ratio of heterotrophic-hydrocarbonoclastic bacteria was used to follow the evolution in time of both populations. Numerical taxonomic methods were employed in order to define ecotypes of marine oil degraders.
Tipo: Text Palavras-chave: Pollution monitoring; Blowouts; Bacteria; Heterotrophic organisms; Biodegradation; Oil pollution.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1019.pdf
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Nocivite relative de cinq detergents anioniques en milieu marin. 1. Toxicite aigue a l'egard de quinze organismes ArchiMer
Maggi, Pierre; Cossa, Daniel.
The acute toxicity of 5 anionic detergents towards 15 marine organisms is related in this study which indicates that fish are very susceptible while Crustacea are the most resistant animals. Phytoplankton and molluscs appear to have intermediate sensibility tothose syndets. The lethal concs of the 5 anionic detergents used in this experiences towards the most susceptible marine spp are near one mgm/l.
Tipo: Text Palavras-chave: Anguilla anguilla; Toxicity; Biodegradation; Anionic detergent.
Ano: 1973 URL: http://archimer.ifremer.fr/doc/1973/publication-2154.pdf
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Nocivite relative de cinq detergents anioniques en milieu marin. 2. Relation entre la biodegradation et la toxicite aigue ArchiMer
Cossa, Daniel; Maggi, Pierre.
In this research, biodegradation associated with evaluation of the variations of acute toxicity of 5 anionic detergents towards Anguilla anguilla is described. It appears that the process of anionic detergent biodegradation in sea water is similar to the results obtained by many authors working on fresh water. The experimentation shows 2 different kinds of behavior among the 5 anionic detergents tested: (a) the first category is composed of syndets, without aryl group in their formulation, which are promptly oxidized by bacteria and do not reveal variation of acute toxicity during their biodegradation; (b) the other group includes LAS; those syndets need a quite long bacterial acclimation. On the other hand, their toxicity is reduced whilst they are...
Tipo: Text Palavras-chave: Anguilla anguilla; Toxicity; Biodegradation; Anionic detergent.
Ano: 1973 URL: http://archimer.ifremer.fr/doc/1973/publication-2155.pdf
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Plasmid-associated phenathrene degradation by Chesapeake Bay sediment bacteria. ArchiMer
Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R.
A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene.
Tipo: Text Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf
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