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RESPUESTA DE LA CLOROFILA Y EL METABOLISMO DE UN ARROYO ANDINO AL AUMENTO DE TEMPERATURA EN UN EXPERIMENTO ex situ Acta biol.Colomb.
INFANTE CANGREJO,Viviana; DONATO RONDÓN,Jhon Charles.
RESUMEN En los trópicos, el cambio climático puede significar un incremento en la temperatura que puede afectar la diversidad biológica de los ecosistemas fluviales, favoreciendo la llegada de especies propias de aguas más cálidas. La variación de temperatura puede también afectar las relaciones tróficas y el metabolismo del sistema. En el presente estudio, se analizó el efecto de 2-4 °C de aumento de la temperatura del agua sobre el ensamble de diatomeas y el metabolismo del río Tota (alta montaña andina). Para este propósito, se utilizaron canales artificiales de recirculación, en los que diariamente se midieron variables fisicoquímicas (pH, conductividad, temperatura, oxígeno disuelto y velocidad del agua), se recolectaron e identificaron diatomeas, se...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arroyo Neotropical; Biofilm; Cambio climático; Canales recirculantes.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000200008
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Distribución de genes de adhesión y regulación de biofilm en Staphylococcus aureus ABCL
Cruz Martínez,Sofía Francisca; Tapia Pastrana,Gabriela; Castañón Sánchez,Carlos Alberto.
La formación de biofilms es un importante factor de virulencia que contribuye en la cronicidad de los procesos infecciosos producidos por Staphylococcus aureus. Las proteínas presentes en su superficie pueden promover la formación de biofilm. En este estudio se comparó la distribución de genes que codifican para proteínas de adhesión asociadas con la formación de biofilm en cepas resistentes (MRSA) y sensibles a meticilina (MSSA). Al analizar un total de 106 aislados obtenidos de muestras sólidas y líquidas recuperados de un hospital de México, se determinó que la formación de biofilm está asociada de manera significativa a cepas MRSA (83%). Mediante la reacción en cadena de la polimerasa (PCR), se buscó la presencia de nueve genes de adhesinas (eno, ebps,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Biofilm; Resistencia a meticilina; Gen cna; Adhesión.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572016000400020
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Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis Anais da ABC (AABC)
COSTA,GÉSSICA A.; ROSSATTO,FERNANDA C.P.; MEDEIROS,ALINE W.; CORREA,ANA PAULA F.; BRANDELLI,ADRIANO; FRAZZON,ANA PAULA G.; MOTTA,AMANDA DE S. DA.
ABSTRACT The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocin-like substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Crystal violet; Food industry; MTT assay; Peptide P34.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100073
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Histatin 5 and human lactoferrin inhibit biofilm formation of a fluconazole resistant Candida albicans clinical isolate Anais da ABC (AABC)
CURVELO,JOSÉ A.R.; MORAES,DANIEL C. DE; ANJOS,CAMILA A. DOS; PORTELA,MARISTELA B.; SOARES,ROSANGELA M.A..
Abstract:Candida albicans is the most important fungal pathogen that causes infections in humans. Biofilms are hard-to-treat structures due to their high antifungal resistance. Saliva is a fluid that contains antimicrobial substances acting as the first-line of defense against pathogens, and its immune components may be potential tools for the discovery of new treatments against candidiasis. To evaluate the activity of histatin 5 and human lactoferrin against biofilm formation. A fluconazole-resistant Candida albicans clinical isolate was used as the model microorganism. Morphogenesis was evaluated by differential counting. Biofilm quantification was performed by XTT reduction assay. Thickness and topography of biofilms were assessed through confocal laser...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Candida albicans; Fluconazole resistance; Histatin 5; Lactoferrin.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100622
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Sanitizer resistance of biofilm-forming Salmonellaisolated from meat products Arq. Bras. Med. Vet. Zootec.
Milan,C.; Agostinetto,A.; Conceição,R.C.S.; Gonzalez,H.L.; Timm,C.D..
Este estudo avaliou a capacidade de Salmonella enterica subsp. enterica isolada de produtos cárneos formar biofilme e testou sua resistência a diferentes sanitizantes. Vinte cepas foram avaliadas quanto à capacidade de formar biofilme em placas de microtitulação. As cepas formadoras de biofilme foram testadas em superfícies de polietileno de alta densidade, aço inoxidável e vidro e tiveram a sensibilidade ao hipoclorito de sódio e ao iodo avaliada. Duas cepas de Salmonella Enteritidis isoladas de produtos de frango apresentaram capacidade de formar biofilme nas superfícies testadas. Essas cepas alcançaram maiores populações nas superfícies do que aquelas não formadoras de biofilme, e foram mais difíceis de remover ou reduzir. Devido à ação sanitizante ser...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Biofilm; Salmonella; Glass; Stainless steel; High-density.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200642
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Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk Biol. Res.
SOUZA VIANA,ELISETH; MARTINO CAMPOS,MARIA EMILENE; REIS PONCE,ADRIANA; CUQUETTO MANTOVANI,HILÁRIO; DANTAS VANETTI,MARIA CRISTINA.
The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well diffusion techniques, thin layer chromatography (TLC) and detection directly in biofilm with biomonitors. The extracts obtained from planktonic and sessile cell of H. alvei induced at least two of three monitor strains evaluated. The presence of AHLs with up to six carbon atoms was confirmed by TLC. Biofilm formation by H. alvei was inhibited by furanone, as demonstrated by 96-well assay of crystal violet in microtitre plates and by scanning electron microscopy....
Tipo: Journal article Palavras-chave: Acyl homoserines lactones; Biofilm; Furanones; Quorum sensing.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400004
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Resistance of oxidative stress in biofilm and planktonic cells BABT
Jakubowski,Witold; Walkowiak,Bogdan.
This work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stress in bacterial cells in log or stationary phases in both planktonic and biofilm forms. The bacteria were exposed to free-radical donors (H2O2, tBOOH, menadione, SIN-1 or peroxynitrite) in a wide range of final concentrations, from 0.5 to 10mM. Different level of toxicity of individual donors, independence of cell type (planktonic forms or biofilm) and phases of growth were observed. The highest oxidative stress resistance was observed for the cells in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Biofilm; Bacterial colonization; Planktonic cells; Implant.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200300
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Morphological study of biomass during the start-up period of a fixed-bed anaerobic reactor treating domestic sewage BABT
Lima,Cláudio Antonio Andrade; Ribeiro,Rogers; Foresti,Eugenio; Zaiat,Marcelo.
This work focused on a morphological study of the microorganisms attached to polyurethane foam matrices in a horizontal-flow anaerobic immobilized biomass (HAIB) reactor treating domestic sewage. The experiments consisted of monitoring the biomass colonization process of foam matrices in terms of the amount of retained biomass and the morphological characteristics of the cells attached to the support during the start-up period. Non-fluorescent rods and cocci were found to predominate in the process of attachment to the polyurethane foam surface. From the 10th week of operation onwards, an increase was observed in the morphological diversity, mainly due to rods, cocci, and Methanosaeta-like archaeal cells. Hydrodynamic problems, such as bed clogging and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaerobic digestion; Biofilm; Domestic sewage; Fixed-bed reactor; Polyurethane foam.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600020
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Microbial succession within an anaerobic sequencing batch biofilm reactor (ASBBR) treating cane vinasse at 55ºC BABT
Ribas,Maria Magdalena Ferreira; Chinalia,Fabio Alexandre; Pozzi,Eloisa; Foresti,Eugenio.
The aim of this work was to investigate the anaerobic biomass formation capable of treating vinasse from the production of sugar cane alcohol, which was evolved within an anaerobic sequencing batch biofilm reactor (ASBBR) as immobilized biomass on cubes of polyurethane foam at the temperature of 55ºC. The reactor was inoculated with mesophilic granular sludge originally treating poultry slaughterhouse wastewater. The evolution of the biofilm in the polyurethane foam matrices was assessed during seven experimental phases which were thus characterized by the changes in the organic matter concentrations as COD (1.0 to 20.0 g/L). Biomass characterization proceeded with the examination of sludge samples under optical and scanning electron microscopy. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaerobic digestion; Adaptation phase; Wastewater; Sugar cane; Biofilm; Microbiology.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400028
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Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium BABT
Kumar,Muthusamy Ashok; Anandapandian,Kanapathi Thangavel Kasirajan; Parthiban,Karuppiah.
Marine water was used for the biofilm forming bacterial isolation. A biofilm forming device was made by using PVC pipes with sterile glass slides. Five morphologically different bacteria were isolated from the scrapping of glass slides from the device after a month period of time. The efficiency of EPS production was checked for all the isolates. Using scanning electron microscope (SEM), the morphology of microbial cells and colonies was studied. The maximum EPS was produced by the strain B3, which was analyzed and characterized by thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) spectra. B3 EPS displayed a strong absorption band of - OH at 3415.31 cm-1 and COOH at 1631.48 cm-1 showing it to be polysaccharide.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Marine Bacteria; EPS; SEM; TLC and FTIR.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200006
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Immobilized Biofilm in Thermophilic Biohydrogen Production using Synthetic versus Biological Materials BABT
Wongthanate,Jaruwan; Polprasert,Chongrak.
Biohydrogen production was studied from the vermicelli processing wastewater using synthetic and biological materials as immobilizing substrate employing a mixed culture in a batch reactor operated at the initial pH 6.0 and thermophilic condition (55 ± 1ºC). Maximum cumulative hydrogen production (1,210 mL H2/L wastewater) was observed at 5% (v/v) addition of ring-shaped synthetic material, which was the ring-shaped hydrophobic acrylic. Regarding 5% (v/v) addition of synthetic and biological materials, the maximum cumulative hydrogen production using immobilizing synthetic material of ball-shaped hydrophobic polyethylene (HBPE) (1,256.5 mL H2/L wastewater) was a two-fold increase of cumulative hydrogen production when compared to its production using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biohydrogen production; Biofilm; Synthetic material; Biological material; SEM.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100124
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Occurrence of sulphate reducing bacteria (SRB) associated with biocorrosion on metallic surfaces in a hydroelectric power station in Ibirama (SC) - Brazil BABT
Marangoni,Paulo Roberto Dantas; Robl,Diogo; Berton,Marcos Antonio Coelho; Garcia,Carlos Mario; Bozza,Angela; Porsani,Mariana Vieira; Dalzoto,Patricia do Rocio; Vicente,Vânia Aparecida; Pimentel,Ida Chapaval.
The aim of this study was evaluate, two methods for the detection and identification of sulphate reducing bacteria (SRB): ML medium and PCR with specific primers for SRB groups. SRB were detected through the selective medium only on carbon steel, which showed corrosion. Employing specific PCR primer, SBR were detected from all the metallic components assayed, even those that did not present visible corrosion spots, such stainless steel and copper alloys. Despite the presence or absence of corrosion at the later stages effectively by using the selective medium,, the initial stages of the corrosion could only be detected by the amplification of total DNA with SRB specific primers. The early detection of SRB could be employed for preventing the damages on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metallic surface; Hydroelectric power station; Biofilm; Biocorrosion.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500011
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Lab-Scale production of Bacillus atrophaeus' spores by solid state fermentation in fifferent types of bioreactors BABT
Sella,Sandra Regina Barroso Ruiz; Guizelini,Belquis Palácio; Vandenberghe,Luciana Porto de Souza; Medeiros,Adriane Bianchi Pedroni; Soccol,Carlos Ricardo.
Studies were conducted to evaluate Bacillus atrophaeus spores' production by solid-state fermentation (SSF) using sugarcane bagasse as support and soybean molasses as substrate at lab-scale in column bioreactors (forced aeration), plastic bags and Erlenmeyer flasks (aeration by diffusion). Different moisture contents (84%, 86% and 88%; 89%, 91% and 93%) and aeration rates (30mL/min, 45mL/min, 60mL/min and 90mL/min) were studied. The best condition for spore production (3.3x10(10) CFU.g-¹dry matter) in column bioreactor was 80% of initial humidity and no aeration. In Erlenmeyer flasks and plastic bags the best sporulation production reached 1.7 up to 4.7x10(10) CFU.g-1dry matter with 88-93% of initial moisture. The aeration rate had no significant effect on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus atrophaeus; Spores; Solid state fermentation; Column bioreactor; Biofilm.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700021
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Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing BJID
Lima,Jailton Lobo da Costa; Alves,Lilian Rodrigues; Jacomé,Paula Regina Luna de Araújo; Bezerra Neto,João Pacífico; Maciel,Maria Amélia Vieira; Morais,Marcia Maria Camargo de.
ABSTRACT Introduction: Biofilm production is an important mechanism for the survival of Pseudomonas aeruginosa and its relationship with antimicrobial resistance represents a challenge for patient therapeutics. P. aeruginosa is an opportunistic pathogen frequently associated to nosocomial infections, especially in imunocompromised hosts. Objectives: Analyze the phenotypic biofilm production in P. aeruginosa isolates, describe clonal profiles, and analyze quorum sensing (QS) genes and the occurrence of mutations in the LasR protein of non-biofilm producing isolates. Methods: Isolates were tested for biofilm production by measuring cells adherence to the microtiter plates. Clonal profile analysis was carried out through ERIC-PCR, QS genes were by specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biofilm; Multiresistance; Quorum sensing (QS).
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200129
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Microbial investigation of biofilms recovered from endotracheal tubes using sonication in intensive care unit pediatric patients BJID
Ferreira,Thiago de Oliveira; Koto,Rafael Yoshio; Leite,Gabriel Fialkovitz da Costa; Klautau,Giselle Burlamaqui; Nigro,Stanley; Silva,Cely Barreto da; Souza,Ana Paula Idalgo da Fonseca; Mimica,Marcelo Jenne; Cesar,Regina Grigolli; Salles,Mauro José Costa.
Abstract Objectives To compare cultured microorganisms identified on endotracheal tubes biofilms through sonication technique with traditional tracheal aspirate collected at extubation of pediatric intensive care unit patients. Methods Demographic and epidemiological data were analyzed to identify factors possibly related with the microbiological profile of the two collection methods. Associations between categorical and continuous variables were analyzed using the chi-square or Fisher's exact test, or Student's t test. p-Value <0.05 were considered significant. Results Thirty endotracheal tubes and tracheal aspirates samples from 27 subjects were analyzed. Only one patient presented the clinical diagnosis of ventilator-associated pneumonia. Overall,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sonication; Endotracheal tube; Biofilm; Tracheal aspirate.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500468
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Influence of iron deprivation on virulence traits of mycobacteria BJID
Pal,Rahul; Hameed,Saif; Sharma,Sharda; Fatima,Zeeshan.
ABSTRACT Novel strategies to combat the ever increasing burden of drug resistance in Mycobacterium tuberculosis (MTB) causing tuberculosis (TB) remains a global concern. The ability of MTB to sense and adapt to restricted iron conditions in the hostile environment is essential for their survival and confers the basis of their success as dreadful pathogen. The striking and clinically relevant virulence trait of MTB is its ability to form biofilms and adhere to the host cells. The present study elucidated the effect of iron deprivation on biofilm formation and cell adherence of Mycobacterium smegmatis, a non-pathogenic surrogate of MTB. Firstly, we showed that iron deprivation leads to enhanced cell sedimentation rate and altered colony morphology depicting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron; Morphology; Biofilm; Anti-TB drugs.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600585
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Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms BJID
Sun,Erlin; Liang,Gehong; Wang,Lining; Wei,Wenjie; Lei,Mingde; Song,Shiduo; Han,Ruifa; Wang,Yubao; Qi,Wei.
Abstract Aims We sought to characterize the antibiotic susceptibility of strains of Stenotrophomonas maltophilia isolated from clinical samples, and the role of Stenotrophomonas maltophilia biofilm in antibiotic resistance. Methods Fifty-one clinical Stenotrophomonas maltophilia isolates were obtained from patients with nosocomial infection in the surgical wards and ICUs of six general hospitals in Tianjin, China. In vitro models of Stenotrophomonas maltophilia biofilms were established and confirmed by scanning electron microscopy and fluorescence microscopy with silver staining. The minimal inhibitory concentrations and biofilm inhibitory concentrations of commonly used antibiotics were determined. Results 47 of 51 strains were resistant to three or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stenotrophomonas maltophilia; Biofilm; Antibiotic resistance; Nosocomial.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000400365
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Modulation of drug resistance and biofilm formation of Staphylococcus aureus isolated from the oral cavity of Tunisian children BJID
Zmantar,Tarek; Ben Slama,Rihab; Fdhila,Kais; Kouidhi,Bochra; Bakhrouf,Amina; Chaieb,Kamel.
Abstract Objectives: This study aims to investigate the antimicrobial and the anti-biofilm activities of Lactobacillus plantarum extract (LPE) against a panel of oral Staphylococcus aureus (n = 9) and S. aureus ATCC 25923. The in vitro ability of LPE to modulate bacterial resistance to tetracycline, benzalchonium chloride, and chlorhexidine were tested also. Methods: The minimum inhibitory concentrations (MICs) and the minimal bactericidal concentrations of Lactobacillus plantarum extract, tetracycline, benzalchonium chloride and clohrhexidine were determined in absence and in presence of a sub-MIC doses of LPE (1/2 MIC). In addition, the LPE potential to inhibit biofilm formation was assessed by microtiter plate and atomic force microscopy assays....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial; Staphylococcus aureus; Biofilm; Antibiotics; Synergetic; Lactobacillus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100027
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Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies BJID
Brilhante,Raimunda Sâmia Nogueira; Caetano,Erica Pacheco; Oliveira,Jonathas Sales de; Castelo-Branco,Débora de Souza Collares Maia; Souza,Elizabeth Ribeiro Yokobatake; Alencar,Lucas Pereira de; Cordeiro,Rossana de Aguiar; Bandeira,Tereza de Jesus Pinheiro Gomes; Sidrim,José Júlio Costa; Rocha,Marcos Fábio Gadelha.
ABSTRACTThe antifungal activity of some statins against different fungal species has been reported. Thus, at the first moment, the in vitro antifungal activity of simvastatin, atorvastatin and pravastatin was tested againstCandida spp. and Cryptococcus spp. Then, in a second approach, considering that the best results were obtained for simvastatin, this drug was evaluated in combination with antifungal drugs against planktonic growth and tested against biofilms ofCandida spp. and Cryptococcus spp. Drug susceptibility testing was performed using the microdilution broth method, as described by the Clinical and Laboratory Standards Institute. The interaction between simvastatin and antifungals against planktonic cells was analyzed by calculating the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida; Cryptococcus; Simvastatin; Antifungal activity; Biofilm.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500459
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Biofilm production using distinct media and antimicrobial susceptibility profile of Pseudomonas aeruginosa BJID
Perez,Leandro Reus Rodrigues; Barth,Afonso Luís.
Biofilm production is an important mechanism for bacterial survival and its occurrence together with antimicrobial resistance represents a challenge for clinical management. Here, we evaluated the ability for biofilm production among P. aeruginosa isolates from patients with or without cystic fibrosis (CF) using two distinct media, besides determining the antimicrobial susceptibility profile of these isolates for eight antimicrobial agents. The ability for biofilm production when TSB medium was used was higher than when used CF sputum media (p = 0.0198). However, P. aeruginosa isolates from CF have demonstrated similar performance for biofilm production, independently of the medium used. Besides, among the biofilm-producing isolates, those recovered from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Pseudomonas aeruginosa; Cystic fibrosis; Antimicrobial resistance.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400001
Registros recuperados: 111
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