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	Provedor de dados BJMBR (1) Electron. J. Biotechnol. (1) Autor Brígido,M.M. (1) Ezaki,Bunichi (1) Kouno,Takafumi (1) Maranhão,A.Q. (1) Yulita,Kusumadewi Sri (1) Palavra-chave Biopanning (2) Anti-nucleic acid antibodies (1) AtGST11 gene (1) DNA binding proteins (1) Phage display (1) ScFv (1) T7 phage differential display (1) Transcription factors (1) Z-DNA (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2010 (1) 2000 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors Electron. J. Biotechnol. Yulita,Kusumadewi Sri; Kouno,Takafumi; Ezaki,Bunichi. Here we introduce a new approach for the screening of DNA binding proteins, using a phage library based on a phage display technique. In principal, a complementary DNA (cDNA) library based on the recombinant bacteriophage T7 expressing target proteins on its capsid (phage display) is constructed. These phage particles are hybridized with a biotinylated target DNA fragment which is immobilized on the surface of streptavidin paramagnetic particle (SA-PMP). The phage particles are released from the target DNA fragment by a nuclease treatment and the recovered phages are used to the next round of hybridization. These processes are repeated three times to amplify the target phages in the population. This simple method is faster, and more systemic than other... Tipo: Journal article Palavras-chave: AtGST11 gene; Biopanning; DNA binding proteins; T7 phage differential display; Transcription factors. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100014 Expression of anti-Z-DNA single chain antibody variable fragment on the filamentous phage surface BJMBR Maranhão,A.Q.; Brígido,M.M.. We describe the expression of an anti-Z-DNA single chain variable region antibody fragment (scFv) on a filamentous phage surface. Four vectors for phage display were constructed. Two of them are able to display multiple copies of the antibody fragment, and the others can be used to make monovalent libraries. The vectors use different promoter/leader sequences to direct the expression of the fused proteins. All were able to promote the assembly of fusion virion particles. In this paper we also show the affinity selection (biopanning) of those phage-antibodies based on the capacity of their products to recognize the antigen. We used biotinylated Z-DNA and the selection was performed in a solution phase fashion. The data presented here indicate that these... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anti-nucleic acid antibodies; Phage display; Biopanning; ScFv; Z-DNA. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500012 Registros recuperados: 2 Primeira ... 1 ... Última

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