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การผลิตโพลีโคลนอลแอนติซีรัมที่จำเพาะต่อ Streptomyces-PR15 และการใช้ตรวจติดตามเชื้อรอบผิวรากต้นกล้าพริก Thai Agricultural
Weerakorn Saengsai; Petcharat Thummabenjapone.
The antagonistic Streptomyces-PR15 is a broad spectrum against various plant pathogenic fungi and bacteria of economic crops. To develop the ELISA technique for detection the Streptomyces-PR15 in bioproducts and plant surrounding environment, the production of polyclonal antiserum against Streptomyces-PR15 was carried out. The antigen prepared from Streptomyces-PR15 whole cell proteins was mixed with Freund’s incomplete adjuvant (1:1, v/v) was injected to in New Zealand White rabbit with immunization program as follows, intramuscular injection for 3 times then only antigen was intravenous injected for 2 times. Bleeding was done at 10 days after final immunization and then at weekly interval for 8 weeks. The antigen was re-intravenous injected at 14 days...
Tipo: PhysicalObject Palavras-chave: Pepper; Chilli seedlings; Antagonist; Streptomyces-PR15; Polyclonal antiserum; Bioproduct; Elisa; Antiserum against; พริกหนุ่มเขียว; พริกขี้หนู; รากต้นกล้า; จุลินทรีย์ปฏิปักษ์; เชื้อแบคทีเรีย สเตรปโตมัยซีส; โพลีโคลนอลแอนติบอดี; การตรวจติดตามเชื้อ; ชีวภัณฑ์; คุณภาพแอนติซีรัม; แอนติบอดีจำเพาะ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5804
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Selection, isolation, and identification of fungi for bioherbicide production BJM
Souza,Angélica Rossana Castro de; Baldoni,Daiana Bortoluzzi; Lima,Jessica; Porto,Vitória; Marcuz,Camila; Machado,Carolina; Ferraz,Rafael Camargo; Kuhn,Raquel C.; Jacques,Rodrigo J.S.; Guedes,Jerson V.C.; Mazutti,Marcio A..
Abstract Production of a bioherbicide for biological control of weeds requires a series of steps, from selection of a suitable microbial strain to final formulation. Thus, this study aimed to select fungi for production of secondary metabolites with herbicidal activity using biological resources of the Brazilian Pampa biome. Phytopathogenic fungi were isolated from infected tissues of weeds in the Pampa biome. A liquid synthetic culture medium was used for production of metabolites. The phytotoxicity of fungal metabolites was assessed via biological tests using the plant Cucumis sativus L., and the most promising strain was identified by molecular analysis. Thirty-nine fungi were isolated, and 28 presented some phytotoxic symptoms against the target plant....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioprospecting; Diaporthe; Bioproduct; Liquid fermentation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100101
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