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Bacillus EFFECT ON THE GERMINATION AND GROWTH OF TOMATO SEEDLINGS (Solanum lycopersicum L) Acta biol.Colomb.
CABRA CENDALES,Teresa; RODRÍGUEZ GONZÁLEZ,Cristian Alonso; VILLOTA CUÁSQUER,Claudia Patricia; TAPASCO ALZATE,Omar Alberto; HERNÁNDEZ RODRÍGUEZ,Annia.
ABSTRACT The capacity to solubilize phosphate and to produce indole compounds Indole Acetic Acid type, was evaluated in 15 strains isolated from castor bean lignocellulosic residues (Ricinus communis). To determine the solubilizing activity of phosphates a qualitative test by using Pikovskaya culture medium was employed and for the evaluation of the production of indole compounds (IAA) a Salkowsky colorimetric analysis technique was applied. Among the microorganisms tested, the Bacillus pumilus GIBI 206 demonstrated capacity to solubilize phosphates and Bacillus subtilis GIBI 200 showed of capacity to solubilize phosphates and to produce Indoleacetic Acid (IAA). To determine the effect of the Bacillus subtilis strain on germination and growth promotion,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; Indole; PGPB; Seedlings; Tomato.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000100004
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Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant Acta Botanica
Magioli,Claudia; Mansur,Elisabeth.
Eggplant is an agronomically important non-tuberous solanaceous crop grown primarily for its large oval fruit. In popular medicine, eggplant is indicated for the treatment of several diseases, including diabetes, arthritis, asthma and bronchitis. Eggplant is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by hybridizing eggplant with wild resistant Solanum species, which present a wide genetic diversity and are source of useful agronomic traits. The application of in vitro methodologies to eggplant has resulted in considerable success. Eggplant tissues present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum melongena L.; Biotechnology; Tissue culture; Genetic transformation; Model plant.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062005000100013
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Performance of beef cattle bulls in feed lots and fed on diets containing enzymatic complex Animal Sciences
Oliveira, Leonardo Guimarães de; Ferreira, Reginaldo Nassar; Padua, João Teodoro; Ulhoa, Cirano José; Cysneiros, Cristine dos Santos Settimi; Arnhold, Emmanuel.
Current paper evaluates the performance of confined beef cattle supplemented with amylolytic enzyme complex produced by fungus Aspergillus awamori and a commercial product containing multienzyme complex, yeast and MOS. Treatments comprised control (basal diet composed of 16% Mombasa grass silage, 66% ground corn, 3% vitamin nuclear mineral and 15% cottonseed meal), amylase treatment (control diet with the addition of 48.7 saccharifying units kg-1 diet) and compound treatment (control diet with the addition of enzymatic complex composed of 83.2 saccharifying units, 8.8 fibrolytic units, 0.05 g of mannan oligosaccharides and 0.2 g of inactivated yeast Kg-1 of the dry matter diet). The addition of products did not significantly increase daily weight gain,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.04.03.02-8 starch; Biotechnology; Feedlot; Ruminant..
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/26446
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Effects of temperature and time on oil extraction from some Nigerian indigenous fresh water microalgae species CIGR Journal
Asoiro, Felix Uzochukwu; Okonkwo, Wilfred Ifeanyi; Akubuo, Clement Onyeaghala; Nweze, Nkechinyere O.; Anyanwu, Cosmas Ngozichukwu.
This study determined the effects of temperature and time on oil extraction from indigenous freshwater microalgae– Dictyosphaerium, Chlorella, Desmodesmus and Cosmarium species, cultured in fifteen 2-litre column photobioreactors (PBR) (three per specie).  Growth and specific growth rates for the mixotrophic>autotrophic cultivation of the species. Oil was extracted from the dried microalgae species at temperatures ranging from 40 to 120 °C at 20 oC intervals and times ranging from 30 to 210 minutes at 30 minutes’ intervals, by accelerated solvent extraction method. Extraction temperature, time and type of microalgae species had significant effect (p < 0.05) on oil yield (temperature > time > type of species).  As extraction temperature and time...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural engineering; Bioresources engineering; Biotechnology; Energy; Renewable energy; Agriculture. oil yield; Dictyosphaerium spp; Desmodesmus subspicatus; Chlorella lewinii; Cosmarium spp; Growth rate..
Ano: 2019 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5247
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Effectiveness of both synthetic compounds and biological extracts against powdery mildew (Blumeria graminis f. sp. tritici) on winter wheat Agrociencia
Věchet,Lubomír; Šerá,Božena.
The objective of this study was to evaluate the effectiveness of foliar application of various synthetic compounds and biological extracts against powdery mildew on wheat. Seed coats of winter wheat were treated by synthetical compounds (benzothiadiazole, salicylic acid, and glycine betaine) or by the extracts from plants (oak, giant knotweed, curcuma, and ginger). Then the seeds were planted using small-field experiments with powdery mildew infection (repetitions over a two-year period; experimental field in Ruzyne, Prague). Leaf area infection was measured and the Cumulative Proportion of Leaf Area Diseased (CPLAD) was calculated. Data were analysed using ANOVA (Tukey test, p≤0.05). Effectiveness of preparations varied 30 % to 72 % in 2008, and 25 % to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; Curcuma; Giant knotweed; Ginger; Infection; Oak.
Ano: 2015 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000100006
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Intellectual property rights related to the genetically modified glyphosate tolerant soybeans in Brazil Anais da ABC (AABC)
Rodrigues,Roberta L; Lage,Celso L. S; Vasconcellos,Alexandre G.
The present work analyzes the different modalities of protection of the intellectual creations in the biotechnology agricultural field. Regarding the Brazilian legislations related to the theme (the Industrial Property Law - no. 9. 279/96 and the Plant Variety Protection Law - no. 9. 456/97), and based in the international treaties signed by Brazil, the present work points to the inclusions of each of them, as well as to their interfaces using as reference the case study of glyphosate tolerant genetically modified soybean. For this case study, Monsanto's pipelines patents were searched and used to analyze the limits of patent protection in respect to others related to the Intellectual Property (IP) laws. Thus, it was possible to elucidate the complex...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; GMO; Intellectual property; Patent; Pipeline; Soybean.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200029
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Comparison of two surgical approaches for Laparoscopic Ovum Pick Up in Ewes Arq. Bras. Med. Vet. Zootec.
Mariano,R.S.G.; Uscategu,R.A.R.; Nociti,R.P.; Barros,F.F.P.C.; Santos,V.J.C.; Coutinho,L.N.; Padilha-Nakaghi,L.C.; Silva,P.D.A.; Teixeira,P.P.M.; Vicente,W.R. R..
ABSTRACT The present study had as an aim to evaluate a right lateral access as an alternative method to laparoscopic ovum pick-up (LOPU) in sheep. Twenty-four Santa Ines ewes were randomly assigned in two groups with twelve animals each: RLD - positioned in right lateral decubitus, with 10º head-down tilt; and DD - positioned in dorsal decubitus with 35º head-down tilt. The following parameters were evaluated every 10 minutes during the procedure: total surgical time (ST), visualized follicles (VF), aspirated follicles (AF), recovered oocytes (RO), mean arterial pressure (MAP), heart rate (HR), respiratory rate (fR) and end tidal CO2 pressure (EtCO2). Pre and postoperative arterial hemogasometry parameters (PaO2, PaCO2, pH, CHCO3 and BE) were also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovine; Surgery; Biotechnology; Genitourinary.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300848
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Synthetic Biology: opportunities for Chilean bioindustry and education Biol. Res.
Federici,Fernán; Rudge,Timothy J; Pollak,Bernardo; Haseloff,Jim; Gutiérrez,Rodrigo A.
In an age of pressing challenges for sustainable production of energy and food, the new field of Synthetic Biology has emerged as a promising approach to engineer biological systems. Synthetic Biology is formulating the design principles to engineer affordable, scalable, predictable and robust functions in biological systems. In addition to efficient transfer of evolved traits from one organism to another, Synthetic Biology offers a new and radical approach to bottom-up engineering of sensors, actuators, dynamical controllers and the biological chassis they are embedded in. Because it abstracts much of the mechanistic details underlying biological component behavior, Synthetic Biology methods and resources can be readily used by interdisciplinary teams to...
Tipo: Journal article Palavras-chave: Synthetic biology; Bioengineering; Education; Engineering; Biotechnology.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400010
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TAPCells, the Chilean dendritic cell vaccine against melanoma and prostate cancer Biol. Res.
Salazar-Onfray,Flavio; Pereda,Cristián; Reyes,Diego; López,Mercedes N.
Here we summarize 10 years of effort in the development of a biomedical innovation with global projections. This innovation consists of a novel method for the production of therapeutic dendritic-like cells called Tumor Antigen Presenting Cells (TAPCells®). TAPCells-based immunotherapy was tested in more than 120 stage III and IV melanoma patients and 20 castration-resistant prostate cancer patients in a series of phase I and I/II clinical trials. TAPCells vaccines induced T cell-mediated memory immune responses that correlated with increased survival in melanoma patients and prolonged prostate-specific antigen doubling time in prostate cancer patients. Importantly, more than 60% of tested patients showed a Delayed Type Hypersensitivity (DTH) reaction...
Tipo: Journal article Palavras-chave: Dendritic Cells; Cancer Immunotherapy; Melanoma Vaccine; Technology Transfer; Biotechnology.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400014
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Sponges in Peru and their potential as a source of antibacterial compounds Boletín de Investigaciones
Cóndor-Luján,Báslavi; Francia-Quiroz,Juan C..
ABSTRACT In the last decades, sponges have emerged as the largest source of active compounds of animal origin for pharmaceutical purposes. In Peru information on these animals is incipient and scattered, which limits taking the right measures for their adequate management, exploitation, and conservation. The aim of this study is to provide an update on the scope and perspectives of sponge research in Peru, with an emphasis on species richness and their antibacterial potential. Likewise, research initiatives carried out in other South American Pacific countries are reviewed. Research efforts in Peru have allowed the description of 46 species; however, the sponge richness is underestimated. On the other hand, in other latitudes, active principles with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Bacteria; Biotechnology; Marine biodiversity; Porifera..
Ano: 2022 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612022000200107
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Bark Extract of Bathysa cuspidata in the Treatment of Liver Injury Induced by Carbon Tetrachloride in Rats BABT
Gonçalves,Reggiani Vilela; Matta,Sérgio Luis Pinto da; Novaes,Rômulo Dias; Leite,João Paulo Viana; Peluzio,Maria do Carmo Gouveia; Vilela,Emerson Ferreira.
The aim of this study was to investigate the effect of bark extract Bathysa cuspidata (BCE) in the reconstitution of hepatic parenchyma and stroma after dysfunction induced by the CCl4. Liver lesions were induced by intraperitoneal administration of CCl4 (1 mL/kg) every 48 h for 12 days. The animals were treated with B. cuspidata extract (BCE) administered by gavage for another 12 days. Forty-nine rats were randomized into seven treatment groups with seven animals receiving CCl4, BCE (200, or 400 mg/kg) and the vehicle DMSO alone, or in different combinations. The extract alone showed no evidence of hepatic toxicity. In general, rats acutely exposed to CCl4 without the treatment with BCE presented high ALT and AST serum levels and high tissue content of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant defense; Biotechnology; Hepatotoxicity; Phytotherapy; Pathology.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400504
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Bioenergetics Potential of RR2 PRO Transgenic Soybean Subjected to Application of Herbicides Isolated and in Combination BABT
Moreno,Giovane; Santos,Viviane Taisa; Albrecht,Leandro Paiola; Cesco,Victor Salomão.
ABSTRACT In Brazil, there are several crops producing oil for biofuel production, such as canola, sunflower, peanut, cotton, castor bean, soybean, among others. There are parameters that indicate the energetic and economic viability for the production of biofuels, and that can be applied, for example, in transgenic soybeans. The present study aimed to evaluate the energetic viability of Intacta RR2 PROTM soybean for biofuel production.Experiments were conducted in the municipalities of Palotina and Marechal Cândido Rondon, both in the State of Paraná. Energy balance calculations were carried out from the production system, estimating energy expenditure, including the agricultural and industrial stages. The inputs used were considered as input of energy,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Bioenergy; Biotechnology; Energy generation; Socioeconomic evaluation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000200229
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Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures BABT
Carone,Sílvia Bicudo; Santa-Catarina,Claudete; Silveira,Vanildo; Floh,Eny Iochevet Segal.
The aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants, explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA and kinetin, under light or in the dark, during successive subcultures. PAs levels followed a basipetal decrease in diploid and an increase in haploid, pith tissues. A similar pattern of total PAs (free + conjugated) was observed for the callus of diploid and haploid plants maintained in the light, and for the haploid callus in the dark, whereas the diploid callus in the dark showed a constant increase in total PAs levels until the end of culture. The PA increase in the diploid callus in the dark was related to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; Nicotiana tabacum; Ploidy; Putrescine; Spermidine; Spermine.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020
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Patent application in biotechnology at subclass C12N in Brazil at the period of 2001 to 2005 BABT
Buttow,Mônica Elisa; Steindel,Mário.
An analysis of Instituto Nacional de Propriedade Industrial (INPI) patent database from 2001 to 2005 showed that less than 5% of the total patent deposited in the subclass C12N, which referred to "Microorganisms or Enzymes, Compositions thereof, Propagating, Preserving or Maintaining Microorganisms, Mutation or Genetic Engineering, Culture Media", were from national depositors. In contrast, more than 34% of all these deposits came from the United States of America during the same period. Among the national depositors, public universities and government research institutions contributed with 83% over all the deposits, demonstrating that research and development in the field of Biotechnology in Brazil has been concentrated within Brazilian public...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Patents; Biotechnology; Intellectual Property; Technologic Innovation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300003
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Date and their processing byproducts as substrates for bioactive compounds production BABT
Tang,Zhen-Xing; Shi,Lu-E; Aleid,Salah Mohammed.
Date is the most popular fruit in middle-east countries. However, the date production process is accompanied by a substantial increase of loss during picking, storage, commercialization and conditioning process. Date and their byproducts have many essential elements for the growth of microorganisms. Thus, they can be converted into value-added compounds through biotechnology. In this paper, date and their processing byproducts used as substrates for producing value-added products such as organic acids, exopolysaccharide, antibiotics, date flavored probiotic fermented dairy, bakery yeast, etc, were reviewed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Date; Byproduct; Biotechnology; Fermentation; Review.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500706
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A conceptual and practical overview of cDNA microarray technology: implications for basic and clinical sciences BJMBR
Mello-Coelho,V. de; Hess,K.L..
cDNA microarray is an innovative technology that facilitates the analysis of the expression of thousands of genes simultaneously. The utilization of this methodology, which is rapidly evolving, requires a combination of expertise from the biological, mathematical and statistical sciences. In this review, we attempt to provide an overview of the principles of cDNA microarray technology, the practical concerns of the analytical processing of the data obtained, the correlation of this methodology with other data analysis methods such as immunohistochemistry in tissue microarrays, and the cDNA microarray application in distinct areas of the basic and clinical sciences.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; CDNA microarray; Gene expression; Genomics.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000011
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Biopharmaceuticals from microorganisms: from production to purification BJM
Jozala,Angela Faustino; Geraldes,Danilo Costa; Tundisi,Louise Lacalendola; Feitosa,Valker de Araújo; Breyer,Carlos Alexandre; Cardoso,Samuel Leite; Mazzola,Priscila Gava; Oliveira-Nascimento,Laura de; Rangel-Yagui,Carlota de Oliveira; Magalhães,Pérola de Oliveira; Oliveira,Marcos Antonio de; Pessoa Jr,Adalberto.
ABSTRACT The use of biopharmaceuticals dates from the 19th century and within 5-10 years, up to 50% of all drugs in development will be biopharmaceuticals. In the 1980s, the biopharmaceutical industry experienced a significant growth in the production and approval of recombinant proteins such as interferons (IFN α, β, and γ) and growth hormones. The production of biopharmaceuticals, known as bioprocess, involves a wide range of techniques. In this review, we discuss the technology involved in the bioprocess and describe the available strategies and main advances in microbial fermentation and purification process to obtain biopharmaceuticals.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biopharmaceuticals; Fermentation process; Biotechnology; Upstream process; Downstream process.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500051
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Isolation and screening of d-limonene-resistant microorganisms BJM
Bicas,Juliano Lemos; Pastore,Gláucia Maria.
This study reports the isolation of microorganisms that are resistant to environment containing limonene, the most important residue in the citrus industry. For the isolation, samples collected from strategic places of a citrus processing plant (yellow water, entrance and exit of the bagasse tank, effluent and deteriorated fruits found in bins, machine straps, fruit washers and plant floor), some citrus fruit and mint from local market were used. The samples were incubated in rotary shaker at 30ºC/150rpm for 48h or 7 days in YM medium containing 0.1% limonene. Great part of the 112 strains recovered after 48h and the 126 strains recovered after 7 days were identified as Gram positive bacilli, followed by Gram negative bacilli, yeasts and Gram positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Limonene; Industrial by-products; Flavor; Biotechnology.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300034
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Production and characterization of tyrosinase activity in Pycnoporus sanguineus CCT-4518 crude extract BJM
Duarte,Lívia Teixeira; Tiba,Joyce Batista; Santiago,Mariângela Fontes; Garcia,Telma Alves; Bara,Maria Teresa Freitas.
Tyrosinase is an enzyme of industrial interest. The production and characterization of tyrosinase from P. sanguineus CCT-4518 were investigated. The selection of inductors, luminosity influence, inoculum size and type of culture medium on the production of tyrosinase and the effect of inhibitors on enzyme activity were performed. Optimum conditions for intracellular tyrosinase production was observed after 2 days using 0.15% L-tyrosine as inducer, in the presence of light, with inoculum size of 10 mycelium discs, using 2% malt extract broth medium, incubated at 30°C, and constant agitation of 150 rpm. Tyrosinase activity was completely inhibited by the addition of 6 mM salicylhydroxamic acid or phenylthiourea, however an inhibition of 4.15% was recorded by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pycnoporus sanguineus; White-rot fungi; Tyrosinase; MBTH; Biotechnology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100003
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Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts from Alternanthera maritima (Amaranthaceae) BJM
Salvador,Marcos J.; Pereira,Paulo S.; França,Suzelei C.; Candido,Regina C.; Ito,Izabel Y.; Dias,Diones A..
The aim of this study was to evaluate the antibacterial and antifungal activity of callus culture (two different hormonal combination culture medium) and adult plants (two collect) extracts from Alternanthera maritima (Amaranthaceae) investigating the maintenance of antimicrobial activity in vivo and in vitro. The antibacterial and antifungal activity was determined by the agar-well diffusion method against thirty strains of microorganisms including Gram-positive and Gram-negative bacteria, yeasts and dermatophytes. All the organic crude extracts studied were bioactive. Extracts of aerial parts and roots of adult plants collected during the same period of years of 1995 and 1998 (Restinga de Maricá (RJ), collect 1 and 2) inhibited the growth of several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternanthera maritima; Adult plants; Callus culture; Biotechnology; Antimicrobial activity.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100022
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