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Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification J. Venom. Anim. Toxins incl. Trop. Dis.
Marassá,A. M.; Rosa,M. D. B.; Gomes,A. C.; Consales,C. A..
The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immunosorbent assay, which was unprecedented for mosquito blood meal identification. The enzyme-linked immunosorbent assay (ELISA) activity was evaluated by the detection of titers on each sample of the 28 blood-fed Culex quinquefasciatus. In light of the high sensitivity that the technique permits, by means of small quantities of specific antibodies commercially provided and phosphatase substrate which reinforces additional dilutions, human and rat blood meals were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotin/avidin sandwich enzyme-linked immunosorbent assay; Culicidae; Blood meal identification.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200008
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