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Santos Jr.,A.R.; Ferreira,B.M.P.; Duek,E.A.R.; Dolder,H.; Wada,M.L.F.. |
Vero cells, a cell line established from the kidney of the African green monkey (Cercopithecus aethiops), were cultured in F-10 Ham medium supplemented with 10% fetal calf serum at 37°C on membranes of poly(L-lactic acid) (PLLA), poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and their blends in different proportions (100/0, 60/40, 50/50, 40/60, and 0/100). The present study evaluated morphology of cells grown on different polymeric substrates after 24 h of culture by scanning electron microscopy. Cell adhesion was also analyzed after 2 h of inoculation. For cell growth evaluation, the cells were maintained in culture for 48, 120, 240, and 360 h. For cytochemical study, the cells were cultured for 120 or 240 h, fixed, processed for histological analysis,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Poly(L-lactic acid); Poly(hydroxybutyrate- co-hydroxyvalerate); Blends; Biomaterials; Cell culture; Vero cells. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100009 |
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Reis,Tamiris Aparecida Diniz dos; Conti,Antonio José de; Barrientos,Eliseo Alexander López; Mori,André Luiz Buzzo; Benassi,Marta de Toledo. |
ABSTRACT Around 20% of Brazilian coffee production corresponds to defective beans (PVA), which decreases the quality of the coffee brews. Steam treatment has been reported as an alternative to improve the cup quality of coffee products. This research aimed to study the feasibility of using steamed Robusta PVA beans in instant coffee products. After the steam treatment (2 bar for 3 min), the Robusta PVA was dried, roasted, subjected to extraction in a pilot plant, and freeze-dried. The steamed and untreated Robusta PVA materials (raw, roasted, and instant coffees) and blends of instant Arabica coffee with PVA were characterized. The steamed Robusta PVA instant coffee presented 7.01 g caffeine and 8.74 g total chlorogenic acids in 100 g product. Instant... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea canephora; Defects; Blends; Bioactive compounds; Steam treatment.. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100248 |
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