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| Lucheis,S. B.; Da Silva,A. V.; Araújo Jr,J. P.; Langoni,H.; Meira,D. A.; Marcondes-Machado,J.. |
| Among domestic animals, dogs are considered to be the major reservoirs of trypanosomatids and, due to their proximity to man, the presence of these parasites in dogs is an alert to actions aiming at triatomine control. Fifty dogs (26 males and 24 females), aged from 2 months to 15 years, belonging to 30 chronic Chagas disease individuals from 15 different municipalities in the western region of São Paulo State, Brazil, were subjected to blood collection for the following tests: artificial xenodiagnosis, blood culture, and Polymerase Chain Reaction (PCR). Forty-three (86%) out of 50 dogs were positive to at least one of the tests performed; 34 (68%) were positive to xenodiagnosis, 30 (60%) to blood culture, and 25 (50%) to PCR for T. cruzi and/or T.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi; T. rangeli; Dog; Xenodiagnosis; Blood culture; Polymerase chain reaction. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400009 |
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| Nascimento-Carvalho,Cristiana M.C.; Lopes,Antônio A.; Gomes,Maria Daniela B.S.; Magalhães,Morgana P.; Oliveira,Juliana R.; Vilas-Boas,Ana Luisa; Ferracuti,Roberta; Brandileone,Maria Cristina C.; Guerra,Maria Luiza L.S.; Alves,Noraney N.; Athayde,Ledilce A.; Caldas,Renilza M.; Barberino,Maria Goreth M. A.; Duarte,Jussara; Brandão,Maria Angélica S.; Rocha,Heonir; Benguigui,Yehuda; Di Fabio,José Luis. |
| Pneumonia is one of the leading causes of hospitalization and death among children in developing countries, and mortality due to pneumonia has been associated with S. pneumoniae infection. This investigation was designed to describe the antimicrobial susceptibility and serotype patterns of pneumococcal strains recovered from the blood of children with community-acquired pneumonia (CAP) and to assess the clinical findings of pneumococcal bacteremic patients with pneumonia. In a 26 month prospective study, blood cultures were obtained as often as possible from children (<16 years of age) diagnosed with CAP in two emergency rooms. Antimicrobial drug susceptibility tests and serotyping were performed when pneumococcus was identified. We studied 3,431 cases... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus pneumoniae; Antimicrobial resistance; Serotypes; Blood culture; Community-acquired pneumonia; Children. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000100003 |
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| Kinoshita-Yanaga,Andréa Tieko; Bértoli,Marta; Martins,Vanessa Aparecida; Mizutani,Angélica Sayuri; Toledo,Max Jean de Ornelas; Araújo,Silvana Marques de; Gomes,Mônica Lúcia. |
| The objective of this study was to evaluate, through blood culture and PCR, the results of the ELISA for Chagas' disease in the screening of blood donors in the public blood-supply network of the state of Paraná, Brazil, and to map the epidemiological profile of the donors with respect to their risk of infection by Trypanosoma cruzi. The negative and positive results of the ELISA were confirmed by blood culture and PCR for 190/191 individuals (99.5%). For one individual (0.5%), the ELISA was inconclusive, blood culture and IIF were negative, and IHA and PCR positive. Three individuals (1.6%) were positive for T. cruzi on all the tests. Donors were predominantly female, and natives of Paraná, of rural origin, had observed or been informed of the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi/detection; ELISA/use for screening; Blood donors; Blood culture; PCR; Blood bank/screened donors. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100007 |
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| Silveira,Marcelo Marques da; Cândido,Stéfhano Luis; Santos,Karin Rinaldi dos; Maia,Maerle Oliveira; Souza,Roberto Lopes de; Sousa,Valéria Régia Franco; Almeida,Arleana do Bom Parto Ferreira de; Dutra,Valeria; Nakazato,Luciano. |
| ABSTRACT: Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood culture; PCR; Bacteremia; Fungemia. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600452 |
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