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| Muller,Maria Viviane Gomes; Petry,André; Vianna,Luciene Pinheiro; Breier,Ana Carolina; Michelin-Tirelli,Kristiane; Pires,Ricardo Flores; Trindade,Vera Maria Treis; Coelho,Janice Carneiro. |
| Gaucher disease is a sphingolipidosis that leads to an accumulation of glucosylceramide. The objective of this study was to develop a methodology, based on the extraction, purification and quantification of glucosylceramide from blood plasma, for use in clinical research laboratories. Comparison of the glucosylceramide content in plasma from Gaucher disease patients, submitted to enzyme replacement therapy or otherwise, against that from normal individuals was also carried out. The glucosylceramide, separated from other glycosphingolipids by high performance thin layer chromatography (HPTLC) was chemically developed (CuSO4 / H3PO4) and the respective band confirmed by immunostaining (human anti-glucosylceramide antibody / peroxidase-conjugated secondary... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glucosylceramide; Blood plasma analysis; Gaucher disease. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400005 |
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