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Use of spectrophotometry as a tool to quantify the sporulation of Penicillium allii in garlic lesions Trop. Plant Pathol.
Valdez,Jorge G.; Piccolo,Ricardo J..
A technique to measure the concentration of Penicillium allii conidia in damp chamber experiments by spectrophotometry was developed. A negative linear correlation (R²=0.56) was observed between transmittance at 340 nm and the concentration of P. allii conidia in water agar 0.05%. The equation that relates transmittance (T) with concentration (conidia mL-1) (y) is: y = 9.3 10(6) - 86497 T. The method was assayed by inoculating 43 P. allii strains in two garlic cultivars. The method proved to be more rapid than the traditional use of a hemocytometer with an improved accuracy. The CV of the number of conidia per hemocytometer reticule was of 35.04%, while the transmittance CV was of 2.73%. The extreme values chosen for T were 40 and 80 because the...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Allium sativum L.; Blue mold; Hemocytometer.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000600010
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Pre- and post-infection activity of new fungicides against Botrytis cinérea and other fungi causing decay of table grapes Ciencia e Investigación Agraria
Serey,Ricardo A; Torres,Rene; Latorre,Bernardo A.
Pre- and post-harvest diseases restrict table grape production and exports (Vitis vinifera L.) in Chile, with the most important disease being grey mold (Botrytis cinérea). In addition, rot due to Aspergillus niger, Cladosporium herbarum, Penicillium expansum, and Rhizopus stolonifer frequently occurs. The pre- and post-infection activity of fungicides against these pathogens was studied on Thompson Seedless table grapes. Detached, mature, berries were used, and inoculations were performed with 20 µL of a 10(6) spores•mL-1 suspension placed on three punctures aseptically made at the calyx end of each berry. Fungicides used (per liter) were boscalid (600 mg), boscalid (200 mg) + pyraclostrobin (100 mg), boscalid (200 mg) + kresoxim methyl (100 mg),...
Tipo: Journal article Palavras-chave: Blue mold; Curative activity; Gray mold; Protection activity; Sour rot; Vitis vinifera.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202007000300005
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