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| Registros recuperados: 74 | |
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| Grizel, Henri. |
| The spread of major diseases of molluscs around the world and the increase of transfers need the development of adapted prophylactic strategies and the increase of research in epidemiology, immunology and genetics. The prophylactic strategies must be based on common laws with the establishment of a list of declarable pathogens, with basic sampling rules (sample size, frequency, definition of geographical areas) and with reglementation of the internal transfers. Moreover, specific adapted diagnosis should be developed to increase the sanitary control performance (e.g. ELISA test, DNA probe, cell culture). In the case of an infectious disease the epidemiology studies should be concerned, the relationship between the culture techniques and the disease, the... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Mollusc culture; Aquaculture techniques; Pathology; Immunology; Genetics; Epidemiology; Fish diseases; Disease control. |
| Ano: 1989 |
URL: http://archimer.ifremer.fr/doc/1989/acte-1483.pdf |
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| Tige, Gilbert; De Kergariou, Gabriel; Cochennec, Nathalie; Rabouin, Marie-agnès. |
| A systematic surveillance progam conducted since 1980 along the coasts of Britanny has followed the evolution and epidemiological state of the natural O. eulis oyster beds and seed areas in the intertidal zones and deep water. Presently, Marteilia refringens exists endemically in Brittany estuaries ; the rate and degree of infestation varies from year to year depending on the hydrological conditions and the geographical region. In no case, however, is the return of flat oyster culture envisoned. Bonamia ostreae is present not only in the estuaries but also in the deep-water rearing areas (Baie de Quiberon, Cancale, Baie de St Brieuc). The infestation increases with age, but also depends on the quantity of the stock and the culture practises. In particular,... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Maladie; Bonamia ostreae; Marteilia refringens; Bretagne; Oyster; Ostrea edulis; Diseases; Bonamia ostreae; Marteilia refringens. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/00022/13299/10353.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf |
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| Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie. |
| A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2... |
| Tipo: Text |
Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf |
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| Robert, Rene; Pichot, Yves; Comps, Michel. |
| From 1988 to 1989, a study has been conducted pertaining to the growth of the flat oyster Ostrea edulis, as well as Marteilia refringens and Bonamia ostreae outbreaks. Commercial mono specific farming (flat oysters), with low density or mixed (flat and hollow oysters) along with higher density, has been taking place in the Arcachon basin oceanographic sectors, where main hydrobiological parameters were acquired simultaneously. The excellent growth of the flat oyster was witnessed in mono specific farming. However this growth was average in mixed farming, probably due to higher total oyster density and interspecies competition. Regardless of the type of farming, Marteilia refringens infection was very quick and may have been related to climatic bay water... |
| Tipo: Text |
Palavras-chave: Basin of Arcachon; Pathology; Bonamia ostreae; Marteilia refringens; Growth; Ostrea edulis; Bassin d'Arcachon; Pathologie; Bonamia ostreae; Marteilia refringens; Croissance; Ostrea edulis. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/1990/rapport-1592.pdf |
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| Touzeau, Damien. |
| L'ostréiculture représente une part économique régionale et française importante avec une production d'environ 117 106 Tonnes de coquillages par an (FAO, 2003). Cependant le développement de cette activité peut être limité par des mortalités, parfois massives, dues à des maladies infectieuses. L'huître plate Ostrea edulis a subi deux maladies majeures : la Marteiliose due à Marteilia refringens et la Bonamiose due à Bonamia ostreae. Ces maladies à déclaration obligatoires ont fait chuter la production de 20 000 tonnes dans les années 70 à 2 098 Tonnes aujourd'hui. Suite à ces mortalités, différents programmes de recherche ont été lancés par Ifremer pour tenter de préserver voire de relancer la culture de l'huître plate. Un de ces programmes concerne la... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Parasite; Bonamia ostreae; Technique PCR; ARN 18 S; Diagnostic. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00034/14478/11777.pdf |
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| Robert, Maeva; Garcia, Celine; Chollet, Bruno; Lopez-flores, Inmaculada; Ferrand, Sylvie; Francois, Cyrille; Joly, Jean-pierre; Arzul, Isabelle. |
| Bonamia ostreae is an intracellular protozoan which is recognized as a cause of mortality in European populations of flat oysters (Ostrea edulis). Based on the recent characterization of actin genes of B. ostreae, specific primers were designed for real-time PCR using SYBR® Green chemistry. Specificity was demonstrated by the unique melting temperature peak observed in positive samples and by the lack of amplification in samples of oysters infected by closely related parasites, including Bonamia exitiosa. A calibration curve using a cloned template was defined to estimate copy number. The assay had a 6 log- dynamic range, mean inter- and intra-assay variation coefficients of <1% and a minimum detection limit of 50 gene copies per reaction. Using... |
| Tipo: Text |
Palavras-chave: Heart imprints; Ostrea edulis; Quantification; Detection; Real time PCR; Bonamia ostreae. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6934.pdf |
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| Bond, Celine. |
| L'huître plate, Ostrea edulis, espèce indigène des côtes européennes est présente de la Norvège à la baie d'Agadir au Maroc ainsi que sur le pourtour méditerranéen et en Mer Noire. Actuellement, la production Française d'huître plate, qui a essentiellement lieu en Bretagne, est de 1 500 tonnes contre 120 000 tonnes pour l'huître creuse Crassostrea gigas. Les travaux développés s'inscrivent dans le cadre de l'étude de la survie du parasite Bonamia ostreae par cytométrie en flux. Des huîtres fortement infectées ont été utilisées pour les purifications de parasites. Les parasites obtenus ont été conservés dans différents milieux et soumis à des conditions environnementales choisies (salinité, température). Les analyses de viabilité des cellules parasitaires... |
| Tipo: Text |
Palavras-chave: Huîtres plates; Ostea edulis; Bonamia ostreae; Survie; Cytométrie en flux; Salinité; Température. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00033/14460/11762.pdf |
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| Arzul, Isabelle; Miossec, Laurence; Blanchet, Estelle; Garcia, Celine; Francois, Cyrille; Joly, Jean-pierre. |
| Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. After its first description in June 1979 in L'Ile Tudy, Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. Bonamiosis and marteiliosis, another protozoan disease appeared in the seventies, drastically reduced the French flat oyster production from 20 000 tonnes in 1970 to less than 2000 tonnes after 1981. In 2001, about 1650 t of flat oysters were marketed in France. The production is restricted to several areas specialised in reproduction, growth and/or marketing, and depends on transfers of animals. Most important production... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Parasitology; Pathology; Ostrea edulis; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-6381.pdf |
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| Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle. |
| The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses.... |
| Tipo: Text |
Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf |
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| Gervais, Ophelie. |
| The flat oyster, Ostrea edulis, is the European endemic oyster species. Its production has been reduced because of overfishing and diseases including bonamiosis. Massive mortalities observed on the Pacific cupped oyster these last years explained the wish of shellfish farmers to diversify their production and their revival of interest for this patrimonial oyster species. However, bonamiosis due to the protozoan, Bonamia ostreae, is still a major problem for the production of this species. Measures to control the disease are limited and require a better knowledge of the interactions between the flat oyster and the parasite. Previous studies have suggested the involvement of apoptosis in flat oyster defense mechanisms against B. ostreae. This mechanism is... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Bonamia ostreae; Apoptose; Flat oyster; Ostrea edulis; Bonamia ostreae; Apoptosis. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00370/48142/48243.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf |
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| Registros recuperados: 74 | |
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