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Karyotype of cryopreserved bone marrow cells BJMBR
Chauffaille,M.L.L.F.; Pinheiro,R.F.; Stefano,J.T.; Kerbauy,J..
The analysis of chromosomal abnormalities is important for the study of hematological neoplastic disorders since it facilitates classification of the disease. The ability to perform chromosome analysis of cryopreserved malignant marrow or peripheral blast cells is important for retrospective studies. In the present study, we compared the karyotype of fresh bone marrow cells (20 metaphases) to that of cells stored with a simplified cryopreservation method, evaluated the effect of the use of granulocyte-macrophage colony-stimulating factor (GM-CSF) as an in vitro mitotic index stimulator, and compared the cell viability and chromosome morphology of fresh and cryopreserved cells whenever possible (sufficient metaphases for analysis). Twenty-five bone marrow...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cryopreservation; Karyotype; Bone marrow cells.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700004
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A safety and feasibility study of cell therapy in dilated cardiomyopathy BJMBR
Martino,H.F.; Oliveira,P.S.; Souza,F.C.; Costa,P.C.; Assunção e Silva,E.; Villela,R.; Gaze,M.; Weitzel,L.H.; Oliveira Jr.,A.; Muccillo,F.B.; Arvelo,S.N.S.; Sá,R.; Guimarães,T.C.F.; Tura,B.R.; Campos de Carvalho,A.C..
The aim of this study was to determine if bone marrow mononuclear cell (BMMC) transplantation is safe for moderate to severe idiopathic dilated cardiomyopathy (IDC). Clinical trials have shown that this procedure is safe and effective for ischemic patients, but little information is available regarding non-ischemic patients. Twenty-four patients with IDC, optimized therapy, age 46 ± 11.6 years, 17 males, NYHA classes II-IV, and left ventricular ejection fraction <35% were enrolled in the study. Clinical evaluation at baseline and 6 months after stem cell therapy to assess heart function included echocardiogram, magnetic resonance imaging, cardiopulmonary test, Minnesota Quality of Life Questionnaire, and NYHA classification. After cell transplantation 1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone marrow cells; Mononuclear fraction; Autologous transplantation; Ejection fraction; Treadmill test; Dilated cardiomyopathy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000011
Registros recuperados: 2
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