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| UESAKA, Karin; MAEZAWA, Masaki; INOKUMA, Hisashi; 猪熊, 壽. |
| A serological survey of Borrelia infection of dogs was performed in Sapporo, Japan, where Borrelia garinii infection in dogs was detected in 2011. A total of 314 serum samples were collected from dogs that visited three animal hospitals in Sapporo from 2012 to 2014. The two-step evaluation method, involving screening ELISA followed by Western blot analysis, was used to detect antibodies against Borrelia species. A total of 34 samples were positive by ELISA. Among those 34 samples, 32 were positive for Borrelia spp. by Western blot. These findings suggest that the 32 dogs (10.2%) generated antibodies against Borrelia burgdorferi sensu lato, such as B. garinii or B. afzelii. Antibody positivity was 7.6% and 13.3% for dogs living in urban and rural areas,... |
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Palavras-chave: Borrelia burgdorferi sensu lato; Canine; Sapporo; Serosurvey. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4277 |
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| Gonçalves,Daniela Dib; Carreira,Teresa; Nunes,Mónica; Benitez,Aline; Lopes-Mori,Fabiana Maria Ruiz; Vidotto,Odilon; Freitas,Julio Cesar de; Vieira,Maria Luísa. |
| The aim of this study was to investigate the presence of DNA of Borrelia burgdorferi sensu lato (s.l.) in ticks that feed on horses used for animal traction in rural Jataizinho, Parana, Brazil. Between February and June 2008, a total of 224 ticks was collected of which 75% were identified as Dermacentor nitens and 25% as Amblyomma cajenense. To amplify B. burgdorferi s.l. DNA, the intergenic space region (ISR) between the 5S (rrf) 23S (rrl) rRNA genes was used as targets for nested-PCR. Two ticks of the D. nitens species were positive for B. burgdorferi s.l. Both species showed a fragment of 184 bp, but the sequencing revealed 99.9% homology with the B. burgdorferi sensu stricto (s.s.) strain B31. These results showed, for the first time, the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ticks; Dermacentor nitens; Borrelia burgdorferi sensu lato; Equine; Rural area. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300035 |
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