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Normal microspore production after cell fusion in Brachiaria jubata (Gramineae) Genet. Mol. Biol.
Mendes-Bonato,Andréa Beatriz; Risso-Pascotto,Claudicéia; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
Cytogenetic studies were carried out on 22 accessions of Brachiaria jubata from the Embrapa Beef Cattle Brachiaria collection. One accession was diploid (2n = 2x = 18) and the remaining 21 were tetraploid (2n = 4x = 36). Among five tetraploid accessions, a specific and constant pattern of cell fusion involving only two microsporocytes was recorded. Meiosis proceeded normally from prophase I to the end, giving rise to an octad with normal microspores that developed into fertile pollen grains. Regular octad formation was possible because each cellular chromosome set was maintained in its proper domain, spindles were correctly positioned, and cytokinesis planes were formed in the correct places. Such behavior of meiosis in syncytes has never been reported in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria jubata; Cell fusion; Microsporogenesis; Pollen fertility.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400016
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Symmetric pollen mitosis I and suppression of pollen mitosis II prevent pollen development in Brachiaria jubata (Gramineae) BJMBR
Risso-Pascotto,C.; Pagliarini,M.S.; Valle,C.B.; Jank,L..
Microsporogenesis and pollen development were analyzed in a tetraploid (2n = 4x = 36) accession of the forage grass Brachiaria jubata (BRA 007820) from the Embrapa Beef Cattle Brachiaria collection that showed partial male sterility. Microsporocytes and pollen grains were prepared by squashing and staining with 0.5% propionic carmine. The meiotic process was typical of polyploids, with precocious chromosome migration to the poles and laggards in both meiosis I and II, resulting in tetrads with micronuclei in some microspores. After callose dissolution, microspores were released into the anther locule and appeared to be normal. Although each microspore initiated its differentiation into a pollen grain, in 11.1% of them nucleus polarization was not observed,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria jubata; Male sterility; Microgametogenesis; Pollen mitosis; Sperm cell.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100006
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