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	Provedor de dados Zoologia (1) BABT (1) BJMBR (1) Genet. Mol. Biol. (1) Autor Fernandez,Maria Aparecida (2) Alves,Maurílio A. R (1) Andrade,Luiz A. M (1) Bravo,Iara S. Joachim (1) Candido-Silva,J.A. (1) Falco,José Ricardo Penteado (1) Mikami,Liya Regina (1) Monesi,N. (1) Polinarski,Celso Aparecido (1) Saccuti,Cecília Ferreira (1) Silva,José Luis da Conceição (1) Soares,Maria Albertina de Miranda (1) Zucoloto,Fernando S (1) Mais... Palavra-chave Bradysia hygida (4) Sciaridae (3) Brazilian flies (1) DNA loops (1) Diptera (1) Eukaryotic Elongation Factor 1A (1) Feulgen reaction (1) Genome size (1) Insect polytene cells (1) Nuclear halo (1) Nuclear matrix (1) Polytene chromosomes (1) Rhynchosciara americana (1) Salivary gland (1) Trichosia pubescens (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/other (1) Ano 2010 (1) 2005 (2) 1993 (1) País Brazil (4) Idioma Inglês (3) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Genome size of three Brazilian flies from the Sciaridae family Genet. Mol. Biol. Saccuti,Cecília Ferreira; Soares,Maria Albertina de Miranda; Falco,José Ricardo Penteado; Fernandez,Maria Aparecida. We determined the genome size of the Brazilian sciarid flies Bradysia hygida, Rhynchosciara americana and Trichosia pubescens (Diptera: Sciaridae) using absorbance measurements of Feulgen-stained nuclei belonging to these species (and chicken erythrocytes as a standard) to calculate the amount of DNA in picograms (pg) and the number of base pairs (bp), or C-value, for each of these species. The C-values were: 3 x 10(8) bp (0.31 pg) for B. hygida; 3.6 x 10(8) bp (0.37 pg) for R. americana; and 1 x 10(9) bp (1.03 pg) for T. pubescens. The sciarids investigated in this work had considerably higher C-values than the average for previously described dipteran species, including D. melanogaster. Tipo: Info:eu-repo/semantics/other Palavras-chave: Genome size; Feulgen reaction; Sciaridae; Bradysia hygida; Rhynchosciara americana; Trichosia pubescens; Brazilian flies. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500015 Aspectos alimentares e de criação de Bradysia hygida Sauaia & Alves (Diptera, Sciaridae) em laboratório Zoologia Bravo,Iara S. Joachim; Alves,Maurílio A. R; Zucoloto,Fernando S; Andrade,Luiz A. M. In nature, the Sciaridae live in moist and shady places, where exists vegetable material in decomposition. It is very dificult to determine the proper Sciaridae's alimentary habit and then, it is dificult, also, to rear Sciaridae species in laboratory. To improvement the Bradysia hygida rearing, the objectives of this research were to analyse the nutritive value of mucuna bean foliage for the larves, to verify the role of the ground (as larvae substrate and in diet composition) and also to verify the possibility of ground substituition by another kind of substrate. Two kinds of sand were employed in the place of the ground and three diets, free of ground, were prepared. The parameters analysed were: duration of the life cicle, porcentage of emergence, egg... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diptera; Sciaridae; Bradysia hygida. Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751993000200012 Nuclear halo from Bradysia hygida (Diptera:Sciaridae) salivary gland polytene cells BABT Polinarski,Celso Aparecido; Silva,José Luis da Conceição; Mikami,Liya Regina; Fernandez,Maria Aparecida. A protocol for recovered nuclear halos from insect polytene nuclei after the extraction of the nuclear proteins using LIS detergent is reported in this work. Analysis was carried out using fluorescence and confocal laser scan microscopy. The extraction of nuclear halos was possible only with nuclei-fraction isolation in hypotonic buffer without spermine and spermidine. The recovered nuclear halos from Bradysia hygida salivary gland polytene nuclei, contributed greatly to the study of the structure and function of these special organelles. Tipo: Info:eu-repo/semantics/article Palavras-chave: Insect polytene cells; Nuclear matrix; Nuclear halo; DNA loops; Salivary gland; Bradysia hygida. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300004 Bradysia hygida (Diptera, Sciaridae) presents two eukaryotic Elongation Factor 1A gene homologues: partial characterization of the eukaryotic Elongation Factor 1A-F1 gene BJMBR Candido-Silva,J.A.; Monesi,N.. Elongation factor 1A is a highly conserved protein that participates in translation. We report the occurrence of two genes homologous to the eukaryotic Elongation Factor 1A in Bradysia hygida and describe the partial cloning and characterization of the B. hygida eukaryotic Elongation Factor 1A-F1 (BheEF1A-F1) gene. The pattern of BheEF1A-F1 expression in the salivary gland at the end of the fourth larval instar was investigated using real-time PCR. The results showed that BheEF1A-F1 expression levels are relatively constant at the time when rapid changes in protein synthesis occur in this tissue. In situ hybridization experiments coupled to Southern blot analyses showed that the BheEF1A-F1 gene is located at position 3d of the A chromosome and a second... Tipo: Info:eu-repo/semantics/article Palavras-chave: Eukaryotic Elongation Factor 1A; Bradysia hygida; Polytene chromosomes; Sciaridae. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500004 Registros recuperados: 4 Primeira ... 1 ... Última

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