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	Provedor de dados BABT (7) Ciênc. Tecnol. Aliment. (1) Autor Tambourgi,Elias Basile (7) Santana,José Carlos Curvelo (5) Fileti,Ana Maria Frattini (3) Lopes,Francisco Luiz Gumes (3) Souza,Roberto Rodrigues de (3) Ferreira,Juliana Ferrari (2) Fischer,Gilvan Anderson (2) Barros,Kleber Vanio Gomes (1) Bresolin,Iara Rocha Antunes Pereira (1) Bresolin,Igor Tadeu Lazzarotto (1) Ehrhardt,Daniela Diniz (1) Fujiki,Tatiana Lie (1) Leite,Manuela Souza (1) Leite,Nadjma Souza (1) Lima,Aloísio André Bomfim de (1) Lédo,Ana da Silva (1) Mazzola,Priscila Gava (1) Santos,Regina Lúcia de Andrade dos (1) Sbruzzi,Dalva (1) Severo Júnior,João Baptista (1) Mais... Palavra-chave Bromelain (8) Pineapple (3) Ananas comosus (2) Enzymatic activity (2) Optimization (2) Reversed micelles (2) Aqueous two-phase systems (1) Characterization (1) Downstream processing (1) Extraction (1) Fieldbus (1) Flat membrane (1) Imperial pineapple (1) Liquid-liquid extraction (1) Medicinal product (1) Membrane separation processes (1) Neural networks (1) PID Controllers (1) Partitioning (1) Pineapple peel (1) Mais... Tipo do documento Info:eu-repo/semantics/article (8) Ano 2013 (1) 2012 (2) 2011 (1) 2010 (2) 2009 (2) País Brazil (8) Idioma Inglês (8)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 8 Primeira ... 1 ... Última The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS BABT Ferreira,Juliana Ferrari; Santana,José Carlos Curvelo; Tambourgi,Elias Basile. In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG4000/phosphate ATPS were done for the pH 6-11 at 25ºC. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed that bromelain... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Aqueous two-phase systems; Partitioning; Characterization; Pineapple. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100017 Neural modeling of bromelain extraction by reversed micelles BABT Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Tambourgi,Elias Basile. A pulsed-cap microcolumn was used for bromelain extraction from pineapple juice by reversed micelles. The cationic micellar solution used BDBAC as the surfactant, isooctane as the solvent and hexanol as the co-solvent. In order to capture the dynamic behavior and the nonlinearities of the column, the operating conditions were modified in accordance with the central composite design for the experiment, using the ratio between the light phase flow rate and the total flow rate, and the time interval between pulses. The effects on the purification factor and on total protein yield were modeled via neural networks. The best topology was defined as 16-9-2, and the input layer was a moving window of the independent variables. The neural model successfully... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Reversed micelles; Extraction; Neural networks; Pineapple. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200026 Concentration by membrane separation processes of a medicinal product obtained from pineapple pulp BABT Lopes,Francisco Luiz Gumes; Severo Júnior,João Baptista; Souza,Roberto Rodrigues de; Ehrhardt,Daniela Diniz; Santana,José Carlos Curvelo; Tambourgi,Elias Basile. The concentration of pineapple juice is needed to retain the bromelain activity and to standardize the composition and proteolytic activity. Thus, this work aimed to obtain a pure bromelain extract from the Ananas comosus L. Merril juice by membrane separation process. A 2² experimental planning was used to study the influence of pH and transmembrane pressure on the activity recovery by micro-filtration using a plain membrane. In second step, this enzyme was purified by the ultra-filtration using a 10 kDa millipore kit. The best operation condition to bromelain concentration using the plain membrane was at pH 7.5 and transmembrane pressure of 0.05 bar, while 85% of bromelain activity was recovered. Ultra-filtration retained 100% of proteolytic activity and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ananas comosus; Bromelain; Membrane separation processes; Purification; Medicinal product. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200024 Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation BABT Leite,Nadjma Souza; Lima,Aloísio André Bomfim de; Santana,José Carlos Curvelo; Lopes,Francisco Luiz Gumes; Lédo,Ana da Silva; Tambourgi,Elias Basile; Souza,Roberto Rodrigues de. This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic activity; Bromelain; Pérola pineapple; Imperial pineapple; Production in vitro. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500002 Viability in the production of a drug extracted from Ananas comosus by a flat membrane system BABT Lopes,Francisco Luiz Gumes; Sbruzzi,Dalva; Barros,Kleber Vanio Gomes; Ferreira,Juliana Ferrari; Santana,José Carlos Curvelo; Souza,Roberto Rodrigues de; Tambourgi,Elias Basile. The aim of this work was to study the production of e bromelain from the Ananas comosus L. Merril, by determining the process conditions using flat membranes. The production system modeling generated a hyperbolical curve and the optimization by response surfaces showed an influence of the transmembrane pressure higher than the pH influence. The cost of the production of bromelain from A. comosus was estimated 9 to 13 times lower than Sigma's retail sales price and 6.5 to 8.5 times lower than when this enzyme was obtained through a liquid-liquid extraction, which showed the economical feasibility of the process. Tipo: Info:eu-repo/semantics/article Palavras-chave: Ananas comosus; Bromelain; Flat membrane; Production cost; Optimization. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300018 Batch and continuous extraction of bromelain enzyme by reversed micelles BABT Fileti,Ana Maria Frattini; Fischer,Gilvan Anderson; Santana,José Carlos Curvelo; Tambourgi,Elias Basile. The main aim of this study was to optimize the conditions for bromelain extraction by reversed micelles from pineapple juice (Ananas comosus). The purification was carried out in batch extraction and a micro-column with pulsed caps for continuous extraction. The cationic micellar solution was made of BDBAC as a surfactant, isooctane as a solvent and hexanol as a co-solvent. For the batch process, a purification factor of 3 times at the best values of surfactant agent, co-solvent and salt concentrations, pH of the back and forward extractions were, 100 mM, 10% v/v, 1 M, 3.5 and 8, respectively. For the continuous operation, independent variables optimal point was determined: ratio between light phase flow rate and total flow rate equal to 0.67 and 1 second... Tipo: Info:eu-repo/semantics/article Palavras-chave: Reversed micelles; Liquid-liquid extraction; Bromelain; Pineapple; Optimization; Surface response. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500021 Isolation and purification of bromelain from waste peel of pineapple for therapeutic application BABT Bresolin,Iara Rocha Antunes Pereira; Bresolin,Igor Tadeu Lazzarotto; Silveira,Edgar; Tambourgi,Elias Basile; Mazzola,Priscila Gava. The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; Pineapple peel; Downstream processing; Waste; Therapeutic applications; Enzymatic activity. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600012 Design of automatic control system for the precipitation of bromelain from the extract of pineapple wastes Ciênc. Tecnol. Aliment. Silva,Flavio Vasconcelos da; Santos,Regina Lúcia de Andrade dos; Fujiki,Tatiana Lie; Leite,Manuela Souza; Fileti,Ana Maria Frattini. In this work, bromelain was recovered from ground pineapple stem and rind by means of precipitation with alcohol at low temperature. Bromelain is the name of a group of powerful protein-digesting, or proteolytic, enzymes that are particularly useful for reducing muscle and tissue inflammation and as a digestive aid. Temperature control is crucial to avoid irreversible protein denaturation and consequently to improve the quality of the enzyme recovered. The process was carried out alternatively in two fed-batch pilot tanks: a glass tank and a stainless steel tank. Aliquots containing 100 mL of pineapple aqueous extract were fed into the tank. Inside the jacketed tank, the protein was exposed to unsteady operating conditions during the addition of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromelain; PID Controllers; Fieldbus. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000400031 Registros recuperados: 8 Primeira ... 1 ... Última

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