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| Registros recuperados: 34 | |
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| Paixão,T.A.; Martinez,R.; Santos,R.L.. |
| Brucelose bovina causada por Brucella abortus é uma importante doença zoonótica, caracterizada pela ocorrência de aborto durante o último trimestre da gestação, o que resulta em diminuição da fertilidade da produção de leite em vacas. A identificação de genes associados à resistência natural contra brucelose tem sido investigada com o objetivo de selecionar animais resistentes à doença. Em bovinos, é controversa a resistência natural contra B. abortus associada ao polimorfismo da região 3' UTR do gene Slc11A1 (Nramp1). Polimorfismos localizados na sequência codificadora de Slc11A1 têm sido identificados em bovinos, contudo a influência sobre a resistência natural contra brucelose não é conhecida. No presente estudo, três novos polimorfismos do gene Slc11A1... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bovine; Brucellosis; Natural resistance; Nramp1; Slc11a1. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400041 |
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| Santos,R.F.; Silva,G.C.P.; Assis,N.A.; Mathias,L.A.. |
| ABSTRACT Bacteria of the genus Brucella are widespread in many countries. These microorganisms can infect humans and many wild and domestic animal species. These bacteria have zoonotic potential, and can cause economic and public health problems since they can be transmitted by direct contact with sick animals, through consumption of contaminated milk, raw meat and its derivatives (Soares et al., 2015). Brucellosis is considered a chronic evolving disease, unusual in horses, predominantly caused by Brucella abortus. However, it is not characterized by reproductive disorders in horses, but primarily by abscess in the cervical region, bursa, tendons, and joints. Transmission is likely to occur via ingestion of contaminated water and pastures, especially in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine; Abattoirs; Brucellosis; Fluorescence polarization; Complement fixation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400853 |
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| Megid,J.; Brito,A.F.; Moraes,C.C.G.; Fava,N.; Agottani,J.. |
| Observou-se brucelose canina em quatro canis diferentes. Os canis apresentaram animais com história de aborto, mortalidade em neonatos e nascimentos prematuros. A porcentagem de animais soropositivos para brucelose canina, pela prova de imunodifusão em ágar gel, variou de 4,6 a 57,1%. Observou-se correlação positiva entre porcentagem de animais positivos e aspectos reprodutivos e condições de aglomeração. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Dog; Brucellosis; Epidemiology; Serology. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500007 |
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| França,S.A.; Mol,J.P.S.; Costa,E.A.; Silva,A.P.C.; Xavier,M.N.; Tsolis,R.M.; Reis,J.K.P.; Paixão,T.A.; Santos,R.L.. |
| Brucella ovis is a major cause of epididymitis in sexually mature rams, resulting in subfertility, infertility, and economic losses for the sheep industry worldwide. The aim of this study was to develop an indirect ELISA (iELISA) using recombinant proteins, namely rBoP59 and rBP26, as antigens for serological diagnosis of B. ovisinfection. The BoP59 and BP26 recombinant proteins were expressed in E. coli and purified by affinity chromatography. Antigenicity was tested by Western blot and iELISA. Standardization of iELISA was performed with 500ng and 1µg BoP59 and rBP26 per well, testing serum from uninfected and experimentally infected rams. rBP26 was effective in distinguishing positive from negative rams. The rBP26 iELISA developed in this study is the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella ovis; Brucellosis; ELISAi; BP26; Western blot. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601695 |
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| Coelho,A.M; Pinto,M.L; Coelho,A.C. |
| In the North of Portugal, a mass vaccination programme of small ruminants was conducted from 2001 to 2004. A study of cost-benefit was carried out for the 2000/2005 period to ascertain the economic benefits of this strategy. In order to estimate the cost of the zoonosis, the compensation costs paid to farmers for culled animals in the Brucellosis Eradication Campaign, data from vaccine Rev. 1 costs, and costs of people internment due to brucellosis were studied. An increase in the cost was observed from 2000 to 2001 (aproximately US$ 110,000), essentially due to compensation for culled animals, but also from vaccination and human internment costs. However, a progressive decrease was observed from 2001 to 2004 (from more than US$1,200,000 in 2001 to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Vaccination; Cost-benefit. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000100001 |
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| Leite,Ricardo de Miranda Henriques; Thompson,James Alan; Gonçalves,Vítor Salvador Picão; Leite,Rômulo Cerqueira; Bandeira,Dimas A.; Lage,Andrey Pereira. |
| A serosurvey was conducted to estimate the prevalence of bovine brucellosis in the State of Paraíba, Brazil. A random sample of six municipalities was selected from each of three ecoregions of the State: the Agreste/Litoral region (1), the Cariri/Curimatau region (2) and the Sertão region (3). These 18 municipalities were divided in quadrants and one herd was selected at random from each quadrant. The sampling scheme included herds with more than 50 bovines, which excludes small-holder subsistence livestock production. At least 32 animals were sampled within each herd, stratified in four age categories, as follows: (1) at least 8 animals under 6 months of age, (2) at least 8 animals of 7 to 18 months of age, (3) at least 8 animals of 19 to 30 months of age... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Epidemiology; Cattle; Paraíba; Brazil. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000900002 |
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| LANGONI,Helio; ICHIHARA,Sílvio Massaru; SILVA,Aristeu Vieira da; PARDO,Renata Bonini; TONIN,Flávia Bechelli; MENDONÇA,Lia Jeanne Pereira; MACHADO,José Aparecido Dorta. |
| Brucellosis is a chronic zoonosis that plays an important role in Public Health. Considering the lack of data in Brazil regarding its presence in raw milk and non-pasteurized dairy products, we determined the presence of brucellae in milk from brucellosis positive animals. The slide agglutination test (SAT), tube agglutination test (TAT) and TAT treated with 2-mercaptoethanol were used to identify positive animals in studied herds. For 3 days, 300 ml milk samples/cow (75 ml/teat) were collected from all productive quarters of the positive animals. These were mixed and centrifuged. Part of the pellet and of the supernatant were inoculated in Farrel and Brodie-Sinton (BS) media supplemented with antimicrobial agents. The inoculated plates and tubes were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Milk; Brucella abortus; Cows; Brucellosis. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600004 |
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| DAFFNER,José; ABALOS,Pedro; PINOCHET,Lautaro; SCORTTI,Mariela; URCELAY,Santiago. |
| Three soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Immunodiffusion tests; Vaccination; Cattle. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000300002 |
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| Ahmed,Rishad; Patil,Basanagouda S.. |
| A 41 year old man presented to the outpatient department with a three month history of difficulty in walking. He also had a history of positive sensory symptoms in the form of pins and needle sensation mostly below the waist. His symptoms had been progressive and there was no significant family history. He demonstrated a spastic gait and could only walk with assistance and support. DTR were hypertonic and sensory deficit was observed below twelfth dorsal vertebra. Sphincter abnormalities were present. Plantars were extensor bilaterally. Cerebral and spinal MRI with contrast was unremarkable. Brucella antigen titers were significantly high. CSF report was consistent with neurobrucellosis. After detailed analysis of his history, clinical picture and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spastic paraparesis; Paraplegia; Brucellosis; Neurobrucella. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300019 |
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| Serefhanoglu,Kivanc; Taskin,Abdullah; Turan,Hale; Timurkaynak,Funda Ergin; Arslan,Hande; Erel,Ozcan. |
| Oxidative stress can be defined as an increase in oxidants and/or a decrease in antioxidant capacity. We aimed to determine total antioxidant capacity (TAC), total peroxide, malondialdehyde and catalase levels in plasma samples, and calculation of oxidative stress index (OSI) in patients with brucellosis to evaluate their oxidative status using a novel automated method. Sixty-nine patients with brucellosis and 69 healthy control subjects were included in the present study. Plasma levels of total peroxide and malondialdehyde were significantly increased in patients as compared with healthy controls (p<0.001 and p<0.001, respectively). In contrast, TAC level was significantly lower in patients as compared with controls (p<0.001). There was no... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Brucellosis; Oxidants; Antioxidants; Oxidative stress. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400001 |
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| Ramos,Taciana Rabelo Ramalho; Pinheiro Junior,José Wilton; Moura Sobrinho,Pedro Alves de; Santana,Vânia Lúcia de Assis; Guerra,Neurisvan Ramos; Melo,Lúcio Esmeraldo Honório de; Mota,Rinaldo Aparecido. |
| The aim of this paper was to study some epidemiological aspects of the infection by Brucella abortus in risk occupational groups in the microregion of Araguaína, Tocantins. For antibody research, 645 serum samples were analyzed by the complement fixation test (CF). A 4.0% frequency was found (26/645) in patients' serum and among those 4.1% (23/551) were slaughterhouses employees and 8.1% (3/37) rural workers. Of the total positive samples, three (2.0%) were women and 23 (4.7%) men; ten (2.9%) were between the ages of 18 and 30, six (3.4%) between 31 and 40, and nine (8.0%) were above 41 years of age. Risk factors for brucellosis in the study groups were age, background (OR = 2.45; CI 95% = 0.98 to 6.10) and previous work conducted with production animals... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Epidemiology; Humans; Risk factors. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200007 |
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| Hasanain,Ahmad; Mahdy,Reem; Mohamed,Asmaa; Ali,Mostafa. |
| Abstract Aim The aim of this study was to compare both the efficacy and safety profile of the WHO-recommended, dual therapy (doxycycline–rifampin) to a quinolone-based, triple therapy (doxycycline–rifampin–levofloxacin) for treating acute/subacute brucellosis. Patients and methods We studied 107 consecutive, naïve patients with acute/subacute brucellosis admitted to Assiut University Hospital. Patients were randomly allocated to receive the dual therapy of doxycycline–rifampin (group-A) or to receive the triple therapy of doxycycline–rifampin–levofloxacin (group-B). Acute/subacute brucellosis was diagnosed based on the presence of: (1) contact with animals or fresh animal products, (2) suggestive clinical manifestations of less than one-year duration,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Doxycycline; Rifampin; Levofloxacin. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300005 |
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| Çiftdoğan,Dilek Yılmaz; Aslan,Selda. |
| Abstract Background and aim Brucellosis is an infectious, contagious and zoonotic disease that occurs worldwide. The family members of an index case of brucellosis may be especially susceptible, due to sharing the same source of infection and similar risk factors for brucellosis. In this study, we propose to screen pediatric and adult family members of brucellosis index cases for detecting additional unrecognized infected family members. Materials and methods 114 family members of 41 pediatric patients with brucellosis were evaluated. All family members completed a brief questionnaire and were tested by a standard tube agglutination test (STA). Results The majority of family members (n = 96, 84.2%) were children. Among the 114 family members, 42 (36.8%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Screening; Unrecognized; Turkey. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500520 |
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| Trangoni,Marcos D.; Gioffré,Andrea K.; Cerón Cucchi,María E.; Caimi,Karina C.; Ruybal,Paula; Zumárraga,Martín J.; Cravero,Silvio L.. |
| In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loop-mediated isothermal amplification; Molecular typing; Brucellosis; Paratuberculosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200619 |
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| Gonçalves,Daniela Dib; Benitez,Aline; Lopes-Mori,Fabiana Maria Ruiz; Alves,Lucimara Aparecida; Freire,Roberta Lemos; Navarro,Italmar Teodorico; Santana,Maria Aparecida Zanella; Santos,Luís Roberto Alves dos; Carreira,Teresa; Vieira,Maria Luísa; Freitas,Julio Cesar de. |
| The aim of this study was to conduct a serological survey for Lyme diseases, brucellosis, leptospirosis and toxoplasmosis and identify the risk variables related to these zoonoses in humans living in the rural area of Jataizinho, state of Parana, Brazil. A total of 63 rural properties were surveyed. Additionally, 207 serum samples collected from these rural area inhabitants were tested for indirect immunofluorescence (IFI) and western blots (WB) were performed to detect Borrelia burgdorferi (sensu lato); a tamponated acidified antigen test (AAT) and 2-mercaptoethanol (2-ME) were used to detect antibodies of Brucella abortus; the microscopic agglutination test (MAT) was carried out to detect antibodies anti-Leptospira spp. and IFI was used to find... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lyme disease; Leptospirosis; Brucellosis; Toxoplasmosis; Human; Antibody. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100018 |
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| Matrone,M.; Keid,L.B.; Rocha,V.C.M.; Vejarano,M.P.; Ikuta,C.Y.; Rodriguez,C.A.R.; Ferreira,F.; Dias,R.A.; Ferreira Neto,J.S. |
| The objective of the present study was to improve the detection of B. abortus by PCR in organs of aborted fetuses from infected cows, an important mechanism to find infected herds on the eradication phase of the program. So, different DNA extraction protocols were compared, focusing the PCR detection of B. abortus in clinical samples collected from aborted fetuses or calves born from cows challenged with the 2308 B. abortus strain. Therefore, two gold standard groups were built based on classical bacteriology, formed from: 32 lungs (17 positives), 26 spleens (11 positives), 23 livers (8 positives) and 22 bronchial lymph nodes (7 positives). All samples were submitted to three DNA extraction protocols, followed by the same amplification process with the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Brucellosis; Abortion; PCR; DNA extraction. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300010 |
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| Registros recuperados: 34 | |
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