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In vitro degradation and gas production of glycerin generated in the biodiesel production chain Animal Sciences
Silva, Livia Soares; Bezerra, Leilson Rocha; Silva, Aderbal Marcos de Azevedo; Carneiro, Heloisa; Moreira, Milenna Nunes; Oliveira, Ronaldo Lopes.
This study was realized to evaluate the production of gas and volatile fatty acids when glycerin was used to replace four forage species through the use of a semi-automated technique in vitro. The experimental design included 4 treatments and 4 replications. The treatments consisted of increasing levels of glycerin (0, 30, 50 and 70%), which was used to replace the forage plants Elephant grass, sugar cane silage, corn silage and Brachiaria grass. Replacement of the Brachiaria and Elephant grasses with glycerin linearly reduced the production of methane and carbon dioxide and ammonia nitrogen and had a positive effect on forage quality (p < 0.05). With the addition of glycerin, the values for acetate concentration decreased (p < 0.05); however, when...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetate; Butyrate; N-NH3; Propionate; VFAs.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/27308
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Effect of replacement of sugarcane by oilseed press cakes in greenhouse gases and volatile fatty acids production in vitro Animal Sciences
Moreira, Milenna Nunes; Silva, Aderbal Marcos de Azevedo; Bezerra, Leilson Rocha; Carneiro, Heloisa; Morais, Raissa Kiara de Oliveira; Medeiros, Fabíola Franklin de.
This study aimed to evaluate the production of methane, carbon dioxide, and volatile fatty acids and changes in ruminal pH in vitro with oilseed press cakes inclusion, such as, cottonseed, sunflower, castor bean, moringa and jatropha at four different levels (0, 30, 50 and 70%) in replacement to the sugarcane in ruminant feeding using semi-automated in vitro technique. The byproduct that produced less CO2 was cottonseed cake (p = 0.0059). The cakes that produced the least amount of CH4 were moringa at 70% (p < 0.05) and cottonseed at 70% levels (p < 0.0001). The cakes that had the highest increases in VFAs were cottonseed and castor (p < 0.0001). Additionally, greater pH was moringa at 70% and cottonseed at 50% levels (p < 0.0001). The greater...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nutrição de Animais Ruminantes acetate; Butyrate; N-NH3; Propionate; VFAs.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/31598
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Stimulation of the Megasphaera elsdenii’s butyrate production in continuous culture by a yeast additive BABT
Soto-Cruz,Oscar; Chávez-Rivera,Rafael; Saucedo-Castañeda,Gerardo.
Continuous culture study of the ruminal bacterium Megasphaera elsdenii using lactate as carbon source is reported. Butyrate, the minor product in batch culture, was the main product in continuous culture. Under steady state conditions the biomass concentration was increased from 0.46 to 0.72 g l-1. Addition of Yea Sacc did not result an increased availability of carbon. The produced amounts of valerate and CO2 were calculated by using a combined carbon and degree of reduction balances. According to these results, the lactate catabolism of M. elsdenii was changed by the presence of yeast additive, yielding more butyrate and diminishing the production of valerate, while acetate and propionate production was not affected. Results reported in this work...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Megasphaera elsdenii; Butyrate; Yeast additive; Continuous culture.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200011
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Butyrate induces apoptosis in murine macrophages via caspase-3, but independent of autocrine synthesis of tumor necrosis factor and nitric oxide BJMBR
Ramos,M.G.; Rabelo,F.L.A.; Duarte,T.; Gazzinelli,R.T.; Alvarez-Leite,J.I..
We demonstrated that 4 mM butyrate induces apoptosis in murine peritoneal macrophages in a dose- and time-dependent manner as indicated by studies of cell viability, flow cytometric analysis of annexin-V binding, DNA ladder pattern and the determination of hypodiploid DNA content. The activity of caspase-3 was enhanced during macrophage apoptosis induced by butyrate and the caspase inhibitor z-VAD-FMK (100 µM) inhibited the butyrate effect, indicating the major role of the caspase cascade in the process. The levels of butyrate-induced apoptosis in macrophages were enhanced by co-treatment with 1 µg/ml bacterial lipopolysaccharide (LPS). However, our data indicate that apoptosis induced by butyrate and LPS involves different mechanisms. Thus, LPS-induced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Butyrate; Apoptosis; Caspase; Nitric oxide; Cytokine; Macrophage.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200004
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