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Purification of C-phycocyanin from Spirulina platensis in aqueous two-phase systems using an experimental design BABT
Antelo,Francine Silva; Costa,Jorge Alberto Vieira; Kalil,Susana Juliano.
C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the conditions of the purification was studied using a central composite rotatable design for each molar mass of PEG. The ATPS composed of 7% (w/w) PEG 1500 or 4% (w/w) PEG 8000 (g/gmol) and 23 or 22.5% (w/w) of phosphate resulted a purification factor of 1.6-fold for C-phycocyanin, with total and 57% recovery, respectively. Process conditions were optimized for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C-phycocyanin; Purification Factor; Protein; Polyethylene glycol.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100001
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