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| Rodrigues,Cristina Crespo; Höfling,José Francisco; Boriollo,Marcelo Fabiano Gomes; Rodrigues,Janaina Aparecida de Oliveira; Azevedo,Ricardo Antunes de; Gonçalves,Reginaldo Bruno; Gomes,Luiz Humberto; Tavares,Flávio César Almeida. |
| The aim of the present research was to evaluate the protein polymorphism degrees among forty-eight C. albicans isolates from fourteen anatomical sites of clinical patients by polyacrylamide gel electrophoresis (SDS-PAGE) and numerical analyzes, in order to identify subspecies and their similarities in some infectious niches. Cell cultures were grown in YEPD medium, collected by centrifugation, and washed in cold saline solution. The whole-cell proteins were extracted by cell disruption using glass beads and submitted to SDS-PAGE technique. After electrophoresis, the protein bands were stained with coomassie-blue and analyzed by statistics package NTSYS-pc version 1.70 software. Similarity matrixes and dendrograms were generated by application of similarity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Numerical analyzes; SDS-PAGE; C. albicans; Human anatomical sites. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100006 |
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| Shinobu,Cristiane Suemi; Ogatta,Sueli Fumie Yamada; Bizerra,Fenando; Furlaneto,Luciana; Peralta,Rosane Marina; Svidzinski,Terezinha Inez Estivalet; Consolaro,Marcia Edilaine Lopes. |
| The physiopathogenesis of vulvovaginal candidiasis (VVC) is still not completely elucidated. The objective of this study was to evaluate if there is a relationship between the different genotypes of Candida albicans, their main agent and the virulence of this yeast in vaginal isolates, and to check if there are laboratorial markers that can predict the ability of each isolate to develop VVC independently of symptoms. The production of exoenzymes protease, phospholipase and haemolysin, resistance to hydrogen peroxide, and the genotype were determined. Genotype A was predominant (75%), protease, phospholipase and haemolytic activity were highly expressed, and the majority of the yeasts were sensitive to H2O2 in 1 and 2 hours of exposure, suggesting that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C. albicans; Vulvovaginal candidiasis; Genotype; Virulence factors. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300015 |
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