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| Demant, Carlos Alberto Rauer; Universidade Estadual Paulista “Julio de Mesquita Filho”; Auld, Dick; Texas Tech University, Lubbock, Texas, United States; Demant, Aline Rodrigues de Mello; Texas Tech University, Lubbock, Texas, United States. |
| In this study, we developed a bioassay to quantify the ricin toxin content of castor bean seeds. Existing quantification methods do not always reflect actual toxicity of the seeds analyzed, which may present lower ricin content even though they are more toxic than seeds presenting a higher content of ricin. This is because these methods actually measure the addition of ricin RCA, which is a compound less toxic than pure ricin. We decided to use in this study, the nematode Caenorhabditis elegans, which has been widely used by the pharmaceutical industry. We tested two strains of C. elegans using different methods in 8 experiments. We examined 4 methods of extracting the ricin complex and 3 methods of exposing the nematodes. Among the nematode strains and... |
| Tipo: Laboratory analysis |
Palavras-chave: Microbiologia e melhoramento genético de plantas 5.01.02.03-6 toxin; C. elegans; Selection; Ricinus microbiologia e melhoramento genetico de plantas. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/11284 |
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| OH,Seung-Il; KIM,Jun-Sung; KIM,Chul-Kyu; YI,Sun Shin; KIM,Sung-Jo; PARK,Sang-Kyu. |
| Abstract It is suggested that oxidative stress induced by cellular reactive oxygen species is one of the major causal factors of aging. The effect of dietary supplementation of anti-oxidants on response to environmental stressors and lifespan has been studied in various model organisms. In the present study, we examine the effect of Artemisia annua extract on resistance to oxidative, heat, and ultraviolet stresses in the nematode Caenorhabditis elegans. Artemisia annua significantly increases survival under oxidative and heat stresses, however has no effects in response to ultraviolet stress. Then, we measured the in vivo changes in expression of stress-responsive genes by Artemisia annua using green fluorescence protein. The expression of hsp-16.2, known... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artemisia annua; Oxidative stress; Heat stress; Lifespan; C. elegans. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000200356 |
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