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Excitatory effects of Buthus C56 toxin on Drosophila larval neuromuscular junction J. Venom. Anim. Toxins incl. Trop. Dis.
Gawade,S. P..
Buthus C56 toxin from venom of the Indian red scorpion Mesobuthus tamulus was studied for its effects on spontaneous miniature excitatory junctional potentials (MEJP) on Drosophila larval neuromuscular junctions. C56 toxin was isolated on CM-Cellulose with linear gradient of ammonium acetate buffer, pH 6.0. Toxin purity was determined on SDS slab gel electrophoresis. Effective concentration of C56 toxin was based on contraction paralysis units (CPU) in Drosophila 3rd instar larvae by microinjection (0.1 CPU/ml = 2 x 10-6 g/ml). The toxin-induced excitatory junctional potentials were studied for calcium dependency (0.2 mM to 1.2 mM Ca2+) in Drosophila Ringer. Excitatory junctional potential amplitude was increased with increasing calcium concentration;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesobuthus tamulus; C56 toxin; Drosophila melanogaster; Neuromuscular; Miniature excitatory junctional potentials (MEJPs).
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100004
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