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	Provedor de dados BJMBR (2) Autor Almeida,D.F. (2) Alves,A.M.B. (2) Ferreira,L.C.S. (2) Lásaro,M.O. (2) Guillobel,H.C.R. (1) Palavra-chave CFA/I fimbriae (2) ETEC (2) DNA vaccine (1) DNA vaccines (1) Diarrheal disease (1) Salmonella bivalent vaccine (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 1999 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última New vaccine strategies against enterotoxigenic Escherichia coli: I: DNA vaccines against the CFA/I fimbrial adhesin BJMBR Alves,A.M.B.; Lásaro,M.O.; Almeida,D.F.; Ferreira,L.C.S.. Stimulation of the mammalian immune system by administration of plasmid DNA has been shown to be an important approach for vaccine development against several pathogens. In the present study we investigated the use of DNA vaccines to induce immune responses against an enteric bacterial pathogen, enterotoxigenic Escherichia coli (ETEC). Three plasmid vectors encoding colonization factor antigen I (CFA/I), an ETEC fimbrial adhesin, were constructed. Eukaryotic cells transfected with each of these plasmids expressed the heterologous antigen in different compartments: bound to the cytoplasmic membrane (pRECFA), accumulated in the cytoplasm (pPolyCFA) or secreted to the outside medium (pBLCFA). BALB/c mice were intramuscularly (im) inoculated with purified... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA vaccines; ETEC; Diarrheal disease; CFA/I fimbriae. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200011 New vaccine strategies against enterotoxigenic Escherichia coli: II: Enhanced systemic and secreted antibody responses against the CFA/I fimbriae by priming with DNA and boosting with a live recombinant Salmonella vaccine BJMBR Lásaro,M.O.; Alves,A.M.B.; Guillobel,H.C.R.; Almeida,D.F.; Ferreira,L.C.S.. The induction of systemic (IgG) and mucosal (IgA) antibody responses against the colonization factor I antigen (CFA/I) of enterotoxigenic Escherichia coli (ETEC) was evaluated in mice primed with an intramuscularly delivered CFA/I-encoding DNA vaccine followed by two oral immunizations with a live recombinant Salmonella typhimurium vaccine strain expressing the ETEC antigen. The booster effect induced by the oral immunization was detected two weeks and one year after the administration of the DNA vaccine. The DNA-primed/Salmonella-boosted vaccination regime showed a synergistic effect on the induced CFA/I-specific systemic and secreted antibody levels which could not be attained by either immunization strategy alone. These results suggest that the combined... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA vaccine; ETEC; CFA/I fimbriae; Salmonella bivalent vaccine. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200014 Registros recuperados: 2 Primeira ... 1 ... Última

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