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Hypomethylation of cytosine residues in cold-sensitive regions of Cestrum strigilatum (Solanaceae) Genet. Mol. Biol.
Guarido,Paula Carolina Paes; Paula,Adriano Alves de; Silva,Carlos Roberto Maximiano da; Rodriguez,Carmen; Vanzela,André Luís Laforga.
Heterochromatin comprises a fraction of the genome usually with highly repeated DNA sequences and lacks of functional genes. This region can be revealed by using Giemsa C-banding, fluorochrome staining and cytomolecular tools. Some plant species are of particular interest through having a special type of heterochromatin denominated the cold-sensitive region (CSR). Independent of other chromosomal regions, when biological materials are subjected to low temperatures (about 0 °C), CSRs appear slightly stained and decondensed. In this study, we used Cestrum strigilatum (Solanaceae) to understand some aspects of CSR condensation associated with cytosine methylation levels, and to compare the behavior of different heterochromatin types of this species, when...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anti-5-methylcytosine; Cestrum; Chromosomes; CMA/DAPI banding; Heterochromatin; Immunolabeling.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300012
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Cytomolecular characterization of cultivars and landraces of wheat tolerant and sensitive to aluminum toxicity Bragantia
Oliveira,Érica Cristina de; Pinto-Maglio,Cecília Alzira Ferreira.
ABSTRACT Karyotyping and chromosome complement knowledge are a prerequisite for cytogenetic mapping. The aim of this study was to characterize 1 common wheat cultivar (BH 1146) and 4 durum wheat genotypes (IAC 1003, Langdon, P19 and P33) from the breeding program of the Agronomic Institute of Campinas (IAC), using karyotype analysis together with Chromomycin A3/4’-6-Diamidino-2-phenylindole (CMA/DAPI) banding techniques for longitudinal characterization of chromosomes and fluorescence in situ hybridization (FISH) in the mapping of the 45S rDNA region. Durum wheat chromosome complement number was 2n = 4x = 28 with the following karyotype formula: 22m + 6sm. Common wheat chromosome complement number was 2n = 6x = 42 with the following karyotype formula: 34m...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Triticum turgidum ssp. durum; Karyotype; CMA/DAPI banding; FISH-rDNA; Cytomolecular mapping.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000400456
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