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| Registros recuperados: 48 | |
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| Leite,Luis Anderson Ribeiro. |
| Molecular tools have been used in taxonomy for the purpose of identification and classification of living organisms. Among these, a short sequence of the mitochondrial DNA, popularly known as DNA barcoding, has become very popular. However, the usefulness and dependability of DNA barcodes have been recently questioned because mitochondrial pseudogenes, non-functional copies of the mitochondrial DNA incorporated into the nuclear genome, have been found in various taxa. When these paralogous sequences are amplified together with the mitochondrial DNA, they may go unnoticed and end up being analyzed as if they were orthologous sequences. In this contribution the different points of view regarding the implications of mitochondrial pseudogenes for entomology... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: COI; Molecular; NUMTs. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300029 |
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| Pires,Amanda Ciprandi; Marinoni,Luciane. |
| The taxonomic crisis, emphasized in recent years, is marked by the lack of popularity (lack of interest in taxonomy) and financial incentives to study biodiversity. This situation, coupled with the issues involved with the necessity of knowing many yet undiscovered species, has meant that new technologies, including the use of DNA, have emerged to revitalize taxonomy. Part of the scientific community, however, has rejected the use of these innovative ideas. DNA barcoding has especially been the target of numerous criticisms regarding its application, as opposed to the use of morphology. This paper aims to highlight the inconsistency of the debate involving DNA versus morphology, since there is a proposal for the integration of traditional taxonomy and DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodiversity; COI; Species; Taxonomic impediment; Morphology. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032010000200035 |
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| Oliveira,Pablo Viana; Matos,Natan Silva; Klippel,Angélica Hollunder; Oliveira-Costa,Janyra; Careta,Francisco de Paula; Paneto,Greiciane Gaburro. |
| ABSTRACT Accurate insect specimen identification is usually a crucial first step in a forensic entomological analysis. It is traditionally done by morphological classification using identification keys. However, due to sensibility limitations in the identification of animal species based only on their morphology, new methods have been developed, including species identification by DNA barcodes. The objective of this study was to identify forensically important species of Diptera in Espirito Santo state using DNA barcodes. For this, adult flies were collected in Espirito Santo, Southeast Region of Brazil. After DNA extraction, COI gene was amplified and sequenced. All sequences were matched to BOLD platform and alternatively to GenBank MegaBLAST. As result,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Entomology; COI; BOLD; Forensic; Flies. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100403 |
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| Guzmán Valencia, Stephanie. |
| La identidad y estructura genética de poblaciones de Oligonychus punicae y O. perseae (Acari: Tetranychidae) fue estudiada en siete localidades productoras de aguacate Persea americana var. Hass. La identidad de los ácaros fue determinada morfológica y molecularmente mediante la secuenciación de una fracción del gen Citocromo Oxidasa subunidad I (COI). La variación genética intraespecífica de diferentes poblaciones de ambas especies de ácaros fue estudiada mediante la secuenciación de las regiones ITS1 e ITS2 del rADN. El análisis filogenético de las secuencias parciales del gen COI separó adecuadamente las dos especies de ácaros, confirmando los resultados de la identificación morfológica. El análisis de las secuencias de las regiones ITS1 e ITS2 del... |
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Palavras-chave: COI; ITS1; ITS2; Estructura genética; Entomología y Acarología; Maestría; Genetic structure. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1765 |
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| Cuellar Sánchez, Valeria Isabel. |
| Diaphorina citri es el vector más importante del Huanglongbing, la enfermedad más destructiva de los cítricos en el mundo. El objetivo de este estudio fue detectar la presencia de variantes morfométricas y genéticas, a través del análisis de secuencias de nucleótidos mediante RAPDs y el gen mitocondrial mtCOI para determinar si existe correspondencia entre variantes de individuos de D. citri Kuwayama (Hemiptera: Liviidae), colectados en rutáceas de Veracruz, México. Se encontraron variaciones morfométricas en el ancho, largo y venación de las alas y de los procesos genales de los especímenes estudiados. Los análisis genéticos por medio de RAPDs y secuencias obtenidas con el gen mtCOI confirman la existencia de cuatro haplotipos (H1-H4) de D. citri... |
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Palavras-chave: Psílido asiático de los cítricos; Morfometría; RAPDs; COI; PCR; Asiatic citrus psyllid; Morphometrics; Entomología y Acarología; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2346 |
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| Paim,Fabilene Gomes; Brandão,José Henrique Souza Galdino; Sampaio,Iracilda; Affonso,Paulo Roberto Antunes de Mello; Diniz,Débora. |
| Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the northeastern coast of Brazil. Sequencing of cytochrome c oxidase subunit I (COI) confirmed all studied samples as S. radians, and all showed high similarity (99-100%) with Caribbean populations. The karyotype of this species was divergent from most marine Perciformes, being composed of 2n = 46 chromosomes. These consisted of a large number of metacentric and submetacentric pairs with small amounts of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: COI; Karyotype; South Atlantic fish; Ribossomal genes; Robertsonian rearrangements. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500007 |
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| Arruda,Cinthya Cristina Bulhões; Beasley,Colin Robert; Vallinoto,Marcelo; Marques-Silva,Nelane do Socorro; Tagliaro,Claudia Helena. |
| Four Brazilian populations of Anomalocardia brasiliana were tested for mutual genetic homogeneity, using data from 123 sequences of the mtDNA cytochrome oxidase c subunit I gene. A total of 36 haplotypes were identified, those shared being H3 (Canela Island, Prainha and Acupe) and both H5 and H9 (Prainha and Acupe). Haplotype diversity values were high, except for the Camurupim population, whereas nucleotide values were low in all the populations, except for that of Acupe. Only the Prainha population showed a deviation from neutrality and the SSD test did not reject the demographic expansion hypothesis. Fst values showed that the Prainha and Acupe populations represent a single stock, whereas in both the Canela Island and Camurupim stocks, population... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MtDNA; COI; Population; Anomalocardia brasiliana; Conservation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200033 |
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| Iketani,Gabriel; Pimentel,Luciana; Silva-Oliveira,Glaúcia; Maciel,Cristiana; Valenti,Wagner; Schneider,Horacio; Sampaio,Iracilda. |
| The giant river prawn, Macrobrachium cf. rosenbergii, is one of the most cultivated freshwater prawns in the world and has been introduced into more than 40 countries. In some countries, this prawn is considered an invasive species that requires close monitoring. Recent changes in the taxonomy of this species (separation of M. rosenbergii and M. dacqueti) require a re-evaluation of introduced taxa. In this work, molecular analyses were used to determine which of these two species was introduced into Brazil and to establish the geographic origin of the introduced populations that have invaded Amazonian coastal waters. The species introduced into Brazil was M. dacqueti through two introduction events involving prawns originating from Vietnam and either... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioinvasion; Exotic species; Giant river prawn; Macrobrachium dacqueti; COI; 16S rRNA; Amazonia. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100025 |
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| Vargas,Jaime; Pérez,Montse; Toro,Jorge; Astorga,Marcela P.. |
| This study presents evidence, using sequences of ribosomal 16S and COI mtDNA, for the presence of two mitochondrial genomes in Perumytilus purpuratus. This may be considered evidence of doubly uniparental mtDNA inheritance. The presence of the two types of mitochondrial genomes differentiates females from males. The F genome was found in the somatic and gonadal tissues of females and in the somatic tissues of males; the M genome was found in the gonads and mantle of males only. For the mitochondrial 16S region, ten haplotypes were found for the F genome (nucleotide diversity 0.004), and 7 haplotypes for the M genome (nucleotide diversity 0.001), with a distance Dxy of 0.125 and divergence Kxy of 60.33%. For the COI gene 17 haplotypes were found for the F... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mytilidae; 16S; COI; Perumytilus; DUI. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200173 |
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| Sousa,Luciene C.C.; Gontijo,Célia M.F.; Botelho,Helbert A.; Fonseca,Cleusa G.. |
| Didelphis albiventris is a well-known and common marsupial. Due to its high adaptability, this very widespread generalist species occurs under various environmental conditions, this even including protected regions and disturbed urban areas. We studied a 653 bp fragment of cytochrome oxidase c (COI) from 93 biological samples from seven Brazilian localities, with linear distances ranging between 58 and about 1800 km to analyze the effects of geographic distances on variability and genetic differentiation. The haplotype network presented nine haplotypes and two genetic clusters compatible with the two most distant geographic areas of the states of Minas Gerais, in the southeast, and Rio Grande do Sul, in the extreme south. As each cluster was characterized... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Didelphis albiventris; Marsupial; Variability; COI; Genetic differentiation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300020 |
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| Machado,Vilmar; Araujo,Aldo M.; Serrano,José; Galián,José. |
| The phylogenetic relationships of twelve species of Chauliognathus were investigated by studying the mitochondrial cytochrome oxidase I gene. A 678 bp fragment of the COI gene was sequenced to test the hypothesis that the Müllerian mimicry species of the "yellow-black" complex make up a monophyletic clade, separated from species with other colour patterns. The data set was analysed by neighbour-joining, maximum parsimony and maximum likelihood procedures. The results support a single origin of the yellow-black colour pattern during the evolution of the genus, with one main clade formed by Chauliognathus lineatus, C. tetrapunctatus, C. riograndensis, C. flavipes, C. octomaculatus, C. fallax, and another one formed by two species, C. expansus and C sp 1,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cantharidae; Chauliognathus; COI; Coleoptera; Müllerian mimicry; Phylogeny; Yellow-black complex. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100010 |
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| Puillandre, N.; Bouchet, P.; Duda, T. F., Jr.; Kauferstein, S.; Kohn, A. J.; Olivera, B. M.; Watkins, M.; Meyer, C.. |
| We present a large-scale molecular phylogeny that includes 320 of the 761 recognized valid species of the cone snails (Conus), one of the most diverse groups of marine molluscs, based on three mitochondrial genes (COI, 16S rDNA and 12S rDNA). This is the first phylogeny of the taxon to employ concatenated sequences of several genes, and it includes more than twice as many species as the last published molecular phylogeny of the entire group nearly a decade ago. Most of the numerous molecular phylogenies published during the last 15 years are limited to rather small fractions of its species diversity. Bayesian and maximum likelihood analyses are mostly congruent and confirm the presence of three previously reported highly divergent lineages among cone... |
| Tipo: Text |
Palavras-chave: Ancestral state reconstruction; Conidae; Conus; COI; 16SrRNA; 12SrRNA. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00468/57920/83480.pdf |
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| Registros recuperados: 48 | |
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